Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   TTHNP4_RS02270 Genome accession   NZ_LR027517
Coordinates   406925..407626 (-) Length   233 a.a.
NCBI ID   WP_124104348.1    Uniprot ID   -
Organism   Thermus thermophilus isolate TTHNAR1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 401925..412626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TTHNP4_RS02235 (TTHN1_00448) - 402068..402724 (+) 657 WP_124104343.1 chromosome segregation protein ScpA -
  TTHNP4_RS02240 (TTHN1_00449) - 402743..403300 (+) 558 WP_124104344.1 Uma2 family endonuclease -
  TTHNP4_RS02245 (TTHN1_00450) - 403334..403696 (-) 363 WP_060384345.1 DUF86 domain-containing protein -
  TTHNP4_RS02250 (TTHN1_00451) - 403683..403982 (-) 300 WP_008631315.1 nucleotidyltransferase family protein -
  TTHNP4_RS02255 (TTHN1_00452) - 404005..404409 (-) 405 WP_353955077.1 Uma2 family endonuclease -
  TTHNP4_RS02260 (TTHN1_00453) - 404597..405022 (-) 426 WP_124104346.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TTHNP4_RS02265 (TTHN1_00454) comZ 405238..406914 (-) 1677 WP_124104347.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  TTHNP4_RS02270 (TTHN1_00455) pilA/pilA3 406925..407626 (-) 702 WP_124104348.1 PilW family protein Machinery gene
  TTHNP4_RS02275 (TTHN1_00456) pilA/pilA2 407623..408129 (-) 507 WP_252973319.1 prepilin Machinery gene
  TTHNP4_RS14420 (TTHN1_00457) - 408699..408962 (+) 264 WP_252973320.1 hypothetical protein -
  TTHNP4_RS02285 (TTHN1_00458) - 409108..409581 (-) 474 WP_124104350.1 GspH/FimT family protein -
  TTHNP4_RS02290 (TTHN1_00459) pilA/pilA1 409637..410107 (-) 471 WP_124104351.1 GspH/FimT family protein Machinery gene
  TTHNP4_RS02295 (TTHN1_00460) - 410265..411245 (+) 981 WP_124104352.1 YpdA family putative bacillithiol disulfide reductase -

Sequence

Protein


Download         Length: 233 a.a.        Molecular weight: 24960.41 Da        Isoelectric Point: 7.4627

>NTDB_id=1115717 TTHNP4_RS02270 WP_124104348.1 406925..407626(-) (pilA/pilA3) [Thermus thermophilus isolate TTHNAR1]
MKRGFTLVEVLVAMAILVVVLAVGVRYFASTSELARNTQARSELQDRVRMVMQVVTADLQMAGARYWNSGNQNQAFSLPL
PPLSGSDMGPKDTLTLYYVTSLRDLASACRRVDYGFEGDTLRRSDVNATPSSGSDCTTPPPNSQPLAEGILALDIQYQCS
DGSRKDTPDCGTDAYPRSAKVTVAGYSLTSVTNPGPASLTTVTGKTLACPQGRACYALTQEVLMPNLKPLPTP

Nucleotide


Download         Length: 702 bp        

>NTDB_id=1115717 TTHNP4_RS02270 WP_124104348.1 406925..407626(-) (pilA/pilA3) [Thermus thermophilus isolate TTHNAR1]
ATGAAGCGCGGGTTCACCCTTGTGGAGGTCCTGGTGGCCATGGCCATCTTGGTGGTGGTCCTGGCCGTGGGCGTGCGCTA
CTTCGCAAGCACCAGCGAGCTCGCCCGGAACACTCAGGCAAGGAGCGAGCTCCAGGACCGGGTGCGCATGGTGATGCAGG
TGGTGACCGCCGATCTCCAGATGGCGGGGGCCCGCTACTGGAATAGCGGCAACCAAAACCAAGCCTTCAGCCTCCCCCTT
CCCCCGCTTTCGGGAAGCGACATGGGCCCTAAGGACACCCTAACCCTCTACTACGTCACGAGCCTCCGGGACCTGGCCTC
CGCCTGCCGCCGGGTGGACTACGGGTTTGAGGGGGACACCCTACGCCGGAGCGACGTGAACGCCACCCCCTCTTCGGGAA
GCGATTGCACCACCCCCCCTCCGAACTCGCAGCCCTTGGCCGAAGGGATCTTGGCCCTGGACATCCAATACCAGTGCAGC
GACGGAAGCCGGAAGGACACGCCCGACTGCGGTACGGACGCCTACCCCCGCTCGGCCAAGGTCACGGTGGCGGGCTACTC
CCTGACCTCGGTGACCAACCCGGGCCCCGCCTCCCTCACCACCGTCACCGGAAAGACCCTCGCCTGCCCCCAGGGCCGGG
CCTGCTACGCCCTCACCCAGGAGGTCCTCATGCCCAACCTCAAGCCCCTGCCCACCCCCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-23)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

99.142

100

0.991

Multiple sequence alignment