Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   A66_RS00150 Genome accession   NZ_LN847353
Coordinates   25238..26599 (+) Length   453 a.a.
NCBI ID   WP_074192812.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain A66     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20238..31599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A66_RS10485 (A66_00021) - 20307..20558 (+) 252 Protein_20 transposase -
  A66_RS10490 - 20593..21390 (-) 798 Protein_21 transposase -
  A66_RS00125 (A66_00025) comW 21656..21892 (+) 237 WP_000939545.1 sigma(X)-activator ComW Regulator
  A66_RS00130 (A66_00026) - 22123..23409 (+) 1287 WP_014931787.1 adenylosuccinate synthase -
  A66_RS00135 (A66_00027) tadA 23610..24077 (+) 468 WP_000291875.1 tRNA adenosine(34) deaminase TadA -
  A66_RS00140 (A66_00028) - 24264..24707 (+) 444 WP_000701992.1 dUTP diphosphatase -
  A66_RS00145 (A66_00029) - 24709..25224 (+) 516 WP_000691236.1 histidine phosphatase family protein -
  A66_RS00150 (A66_00030) radA 25238..26599 (+) 1362 WP_074192812.1 DNA repair protein RadA Machinery gene
  A66_RS00155 (A66_00031) - 26672..27169 (+) 498 WP_001809263.1 carbonic anhydrase -
  A66_RS10500 - 27194..27633 (+) 440 Protein_29 hypothetical protein -
  A66_RS00165 (A66_00034) - 27778..28746 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  A66_RS11515 - 28880..29161 (-) 282 Protein_31 transposase family protein -
  A66_RS12320 - 29288..30195 (-) 908 Protein_32 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49467.06 Da        Isoelectric Point: 6.9524

>NTDB_id=1114549 A66_RS00150 WP_074192812.1 25238..26599(+) (radA) [Streptococcus pneumoniae strain A66]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPHFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFIKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1114549 A66_RS00150 WP_074192812.1 25238..26599(+) (radA) [Streptococcus pneumoniae strain A66]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTAGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTGGCTGAGGTGACTTCAATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTA
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACCCTTCTCCTACA
AGTTTCAACCCAGCTGTCTCAAGTGGGGACTGTTCTCTACGTCAGTGGGGAGGAGTCTGCTCAGCAAATCAAGCTACGAG
CAGAGCGCTTGGGAGATATTGATAGCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGAGCTGAGGTGGAG
CGCATCCAACCACATTTTCTCATCATCGACTCCATCCAGACCATTATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAAGTGCGTGAGGTGACGGCTGAACTTATGCAGCTGGCCAAAACCAATAACATTGCCATCTTTATCGTAGGGCATG
TGACCAAGGAGGGCACTCTGGCTGGTCCGCGTATGTTGGAGCATATGGTGGATACGGTACTTTACTTTGAAGGGGAACGC
CACCATACCTTTCGTATTTTGAGAGCCGTTAAAAACCGTTTTGGCTCCACGAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACGACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCGATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTATTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

99.558

100

0.996
  radA Streptococcus pneumoniae D39

99.558

100

0.996
  radA Streptococcus pneumoniae R6

99.558

100

0.996
  radA Streptococcus pneumoniae TIGR4

99.558

100

0.996
  radA Streptococcus mitis NCTC 12261

99.117

100

0.991
  radA Streptococcus mitis SK321

99.117

100

0.991
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment