Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACMCNJ_RS10710 Genome accession   NZ_AP038788
Coordinates   2340223..2340768 (-) Length   181 a.a.
NCBI ID   WP_226692532.1    Uniprot ID   -
Organism   Rodentibacter sp. THUN1656     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2335223..2345768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMCNJ_RS10685 (THUN1656_20360) - 2335644..2336078 (-) 435 WP_308443650.1 isoprenylcysteine carboxylmethyltransferase family protein -
  ACMCNJ_RS10690 (THUN1656_20370) - 2336118..2336732 (-) 615 WP_226692535.1 YfgM family protein -
  ACMCNJ_RS10695 (THUN1656_20380) hisS 2336751..2338022 (-) 1272 WP_226692534.1 histidine--tRNA ligase -
  ACMCNJ_RS10700 (THUN1656_20390) ispG 2338032..2339138 (-) 1107 WP_226692533.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACMCNJ_RS10705 (THUN1656_20400) - 2339150..2340154 (-) 1005 WP_374707294.1 helix-turn-helix domain-containing protein -
  ACMCNJ_RS10710 (THUN1656_20410) pilF/pilF2 2340223..2340768 (-) 546 WP_226692532.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACMCNJ_RS10715 (THUN1656_20420) - 2340886..2342025 (-) 1140 WP_226692531.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACMCNJ_RS10720 (THUN1656_20430) - 2342260..2343135 (+) 876 WP_226692530.1 D-alanyl-D-alanine carboxypeptidase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20935.59 Da        Isoelectric Point: 8.2406

>NTDB_id=111227 ACMCNJ_RS10710 WP_226692532.1 2340223..2340768(-) (pilF/pilF2) [Rodentibacter sp. THUN1656]
MKPFFIQYLSVVIFPLVFSACVSQTSPADFNKRQAAKARVELALGYLHHNNFPQAKQNLDKALEHDKNYYLVHSAFAHFY
QLQGDVTSARESYLQAIKLNDKQGETYNNFGAFLCGLGEFEQARQAFDRALSSPHYYRQADTYENMALCARAFNQKDVYQ
QAVDKLRQIDEYRAEKLNQIK

Nucleotide


Download         Length: 546 bp        

>NTDB_id=111227 ACMCNJ_RS10710 WP_226692532.1 2340223..2340768(-) (pilF/pilF2) [Rodentibacter sp. THUN1656]
ATGAAACCTTTCTTTATTCAATACTTAAGTGTGGTCATTTTTCCTCTTGTTTTTTCAGCCTGCGTTTCTCAAACTTCCCC
TGCTGATTTCAATAAACGGCAAGCGGCAAAGGCCCGTGTGGAATTAGCTTTAGGCTATCTGCATCACAACAATTTTCCTC
AAGCTAAACAGAATTTAGATAAAGCCTTGGAGCACGATAAAAATTACTACCTTGTGCATTCTGCCTTTGCACATTTTTAT
CAGCTACAAGGCGATGTAACCTCCGCACGGGAATCTTATCTTCAAGCAATAAAACTTAATGATAAACAAGGCGAAACCTA
TAATAATTTCGGTGCCTTTTTATGTGGGCTGGGAGAATTTGAACAAGCCCGCCAAGCCTTTGACCGGGCCTTATCCTCAC
CTCATTATTATCGCCAAGCCGATACTTATGAAAATATGGCGCTTTGCGCACGAGCATTCAATCAAAAGGATGTTTACCAA
CAAGCTGTCGATAAATTACGGCAGATCGATGAATATCGTGCGGAAAAACTGAATCAAATCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(76-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

61.272

95.58

0.586
  pilF Glaesserella parasuis strain SC1401

45.902

100

0.464

Multiple sequence alignment