Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BN5_RS19475 Genome accession   NZ_HG916826
Coordinates   4105449..4106765 (-) Length   438 a.a.
NCBI ID   WP_003463985.1    Uniprot ID   W6R805
Organism   Ectopseudomonas oleovorans CECT 5344 strain CECT5344     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4100449..4111765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN5_RS19440 (BN5_3982) thiS 4100531..4100731 (-) 201 WP_003463964.1 sulfur carrier protein ThiS -
  BN5_RS19445 (BN5_3983) - 4100834..4101205 (-) 372 WP_003463966.1 DUF423 domain-containing protein -
  BN5_RS19450 (BN5_3984) mtgA 4101366..4102094 (+) 729 WP_039965289.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  BN5_RS19455 (BN5_3985) - 4102096..4102707 (-) 612 WP_003463970.1 hypothetical protein -
  BN5_RS19460 (BN5_3986) rpoH 4102770..4103624 (-) 855 WP_003463972.1 RNA polymerase sigma factor RpoH -
  BN5_RS19465 (BN5_3987) ftsX 4103768..4104784 (-) 1017 WP_003463980.1 permease-like cell division protein FtsX -
  BN5_RS19470 (BN5_3988) ftsE 4104784..4105452 (-) 669 WP_003463983.1 cell division ATP-binding protein FtsE -
  BN5_RS19475 (BN5_3989) pilA 4105449..4106765 (-) 1317 WP_003463985.1 signal recognition particle-docking protein FtsY Machinery gene
  BN5_RS19480 (BN5_3990) - 4107104..4107538 (+) 435 WP_039965291.1 BLUF domain-containing protein -
  BN5_RS19485 (BN5_3991) - 4107683..4109050 (+) 1368 WP_003463991.1 pitrilysin family protein -
  BN5_RS19490 (BN5_3992) - 4109047..4110507 (+) 1461 WP_003463993.1 pitrilysin family protein -
  BN5_RS19495 (BN5_3993) rsmD 4110507..4111127 (+) 621 WP_003463996.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -

Sequence

Protein


Download         Length: 438 a.a.        Molecular weight: 46144.97 Da        Isoelectric Point: 5.2212

>NTDB_id=1112131 BN5_RS19475 WP_003463985.1 4105449..4106765(-) (pilA) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
MFGSKDDKNSPAVTDPQAQPVSEEKKSLFSWWRKKPAETTAEPAQPAPVEQPAAQAPVEPPVPEAVATVAVEVAEVAPAP
SASPEPHVVAPPVVAAAVQPQPVVPEPVVEAAPVAAVPAPVAAAATQEKPSFFARLKQGLSKTSASLGEGMASLFLGKKA
IDDDLLDDLETRLLTADVGVEATTAIIGNLTKRVARKELADSGALYKALQEELVTLLKPVEQPLVIDSGKQPYVILVVGV
NGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNNIAVIAQHTGADSASVIFDAVQAAKSRGIDVLIA
DTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNQTVNLTGLALTKLDGTAKGGVIFALA
KQFGLPIRYIGVGEGIDDLRPFQAQAFVQALFEERERG

Nucleotide


Download         Length: 1317 bp        

>NTDB_id=1112131 BN5_RS19475 WP_003463985.1 4105449..4106765(-) (pilA) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
ATGTTTGGTTCCAAAGACGACAAGAACTCACCGGCCGTGACCGACCCCCAAGCCCAGCCCGTGAGCGAAGAAAAGAAATC
CCTGTTCAGCTGGTGGCGCAAGAAACCGGCAGAGACGACTGCCGAGCCTGCTCAGCCTGCGCCCGTTGAGCAACCTGCTG
CTCAGGCGCCTGTCGAGCCGCCCGTGCCCGAAGCCGTAGCAACGGTCGCGGTGGAAGTGGCTGAAGTCGCGCCCGCTCCG
TCAGCGTCGCCTGAGCCGCACGTCGTTGCACCGCCGGTTGTTGCAGCTGCTGTGCAGCCGCAGCCGGTCGTGCCCGAACC
TGTCGTCGAGGCAGCACCCGTGGCGGCAGTACCGGCTCCCGTCGCTGCAGCGGCCACGCAGGAAAAGCCAAGCTTCTTCG
CCCGCCTCAAGCAAGGCCTGTCCAAAACCAGCGCCAGCCTCGGTGAAGGCATGGCCAGCCTGTTCCTCGGCAAGAAGGCC
ATCGACGATGACCTGCTCGACGATCTGGAAACCCGCCTGCTCACCGCCGACGTTGGCGTCGAGGCGACCACCGCGATCAT
CGGCAACCTGACCAAGCGCGTGGCGCGCAAGGAACTGGCCGACAGCGGCGCCCTGTACAAGGCGCTGCAGGAGGAGCTGG
TGACCCTGCTCAAGCCGGTCGAGCAGCCGCTGGTCATCGACAGCGGCAAACAGCCCTATGTGATTCTCGTGGTGGGCGTG
AACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCCAAGAAACTCCAGCTCGAAGGCAAGAAGGTCATGCTCGCCGC
GGGCGACACCTTCCGCGCCGCGGCGGTGGAGCAACTGCAGGTCTGGGGTGAGCGCAACAACATCGCGGTCATCGCCCAGC
ACACCGGTGCCGACTCGGCCTCGGTGATCTTCGATGCGGTGCAGGCCGCCAAGTCGCGTGGCATCGACGTGCTTATCGCC
GACACCGCCGGGCGCCTGCACACCAAAGACAACCTGATGGAAGAGTTGAAGAAGGTCCGTCGGGTGATCGGCAAGCTGGA
CGAAACCGCGCCGCATGAGGTGCTGCTGGTACTCGATGCCGGCACCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCA
ACCAGACCGTGAACCTCACCGGTCTGGCACTGACCAAACTCGACGGCACCGCCAAGGGCGGCGTTATCTTCGCCCTGGCC
AAGCAGTTCGGTCTGCCGATTCGCTATATCGGCGTGGGTGAAGGCATCGATGACCTGCGTCCGTTCCAGGCGCAGGCTTT
CGTCCAGGCACTCTTCGAGGAGCGTGAGCGCGGATGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(233-432)

SRP54-type protein, helical bundle domain (SRP54_N)

(147-213)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W6R805

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.647

97.032

0.482

Multiple sequence alignment