Detailed information NTG1111936

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: PKB_RS23565
• Genome accession: NZ_HG322950
• Coordinates: 5036257..5037594 (+)
• Length: 445 a.a.
• NCBI ID: WP_043255030.1
• Uniprot ID: A0A024HNN1
• Organism: Pseudomonas knackmussii B13
• Function: regulate pilin expression (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5037705..5046992	5036257..5037594	flank	111

Gene organization within MGE regions

Location: 5036257..5046992

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PKB_RS23565 (PKB_4787)	pilR	5036257..5037594 (+)	1338	WP_043255030.1	sigma-54 dependent transcriptional regulator	Regulator
PKB_RS23570 (PKB_4788)	thiO	5037705..5038814 (-)	1110	WP_043255031.1	glycine oxidase ThiO	-
PKB_RS23575 (PKB_4789)	-	5038993..5039505 (+)	513	WP_043255032.1	GspH/FimT family pseudopilin	-
PKB_RS30395 (PKB_4790)	-	5039657..5039866 (+)	210	WP_043257687.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
PKB_RS29355 (PKB_4791)	-	5039918..5040184 (+)	267	Protein_4729	GspH/FimT family pseudopilin	-
PKB_RS23590 (PKB_4792)	pilV	5040175..5040663 (+)	489	WP_052355365.1	type IV pilus modification protein PilV	-
PKB_RS23595 (PKB_4793)	-	5040660..5041352 (+)	693	WP_043255034.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
PKB_RS23600 (PKB_4794)	-	5041349..5041813 (+)	465	WP_043255035.1	PilX N-terminal domain-containing pilus assembly protein	-
PKB_RS23605 (PKB_4795)	-	5041834..5046564 (+)	4731	WP_052355366.1	hypothetical protein	-
PKB_RS29360 (PKB_4796)	-	5046576..5046992 (+)	417	WP_084166710.1	type IV pilin protein	-

Sequence

Protein

• Length: 445 a.a.
• Molecular weight: 49621.83 Da
• Isoelectric Point: 5.8207

>NTDB_id=1111936 PKB_RS23565 WP_043255030.1 5036257..5037594(+) (pilR) [Pseudomonas knackmussii B13]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNIKEAREYLAREPFDLCLTDMRLPDGTGMDLVQHIQQRHPQVPVAM
ITAFGSLDTAVNALKAGAFDFVTKPVDLGRLRELVATALRLRSPSAEEAPVDNRLLGESPPMKQMRNQIAKLARSQAPVY
ISGESGSGKELVARLIHEQGPRSEVAFVPVNCGAIPSELMESEFFGHKKGSFTGAVEDKQGLFQAANGGTLFLDEVADLP
LPMQVKLLRAIQEKAVRAVGGQQEVSVDVRVLSATHKDLAAEVAAGRFRQDLYYRLNVIELRVPPLRERREDIPLLTDRV
LQRLASDSGLAPAKVSADALEKLKSYRFPGNVRELENMLERAYTLCEDDLIQPHDLRFAETASGGESGEASLAQIDNLED
YLEEIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide

• Length: 1338 bp

>NTDB_id=1111936 PKB_RS23565 WP_043255030.1 5036257..5037594(+) (pilR) [Pseudomonas knackmussii B13]
ATGAGCCGGCAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAGCTGCTGGAGATCACCCTCGGCCGCATGAA
GCTCGATACCCGCAGCGCCCGCAACATCAAGGAGGCGCGCGAGTACCTGGCCCGCGAACCCTTCGACCTGTGCCTGACCG
ACATGCGCCTGCCCGACGGCACCGGCATGGACCTGGTGCAGCACATCCAGCAGCGCCACCCACAGGTACCGGTGGCCATG
ATTACCGCCTTTGGCAGCCTGGATACCGCGGTCAACGCGCTCAAGGCCGGCGCCTTCGACTTCGTCACCAAGCCGGTCGA
CCTCGGCCGCCTGCGCGAACTGGTCGCCACCGCGTTGCGCCTGCGTTCGCCGAGCGCCGAAGAAGCACCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGAAGCAGATGCGCAACCAGATCGCCAAGCTCGCGCGCAGCCAGGCGCCGGTGTAC
ATCAGCGGCGAATCGGGTAGCGGCAAGGAGTTGGTGGCACGGCTGATCCACGAACAAGGCCCACGCAGCGAAGTGGCCTT
CGTCCCGGTCAACTGCGGCGCCATCCCGTCAGAGCTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACCG
GCGCCGTGGAAGACAAGCAGGGCCTGTTCCAGGCGGCCAACGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
CTGCCGATGCAAGTCAAGCTGCTGCGCGCCATCCAGGAGAAGGCCGTACGCGCGGTCGGTGGCCAGCAGGAAGTGTCGGT
CGACGTGCGCGTGCTCAGCGCGACACACAAGGACCTCGCTGCAGAAGTCGCCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTGAACGTCATCGAGCTGCGCGTGCCGCCGCTGCGCGAGCGCCGCGAGGATATTCCGCTGCTCACCGATCGCGTG
CTGCAGCGCCTGGCCAGCGATAGCGGCCTGGCGCCGGCGAAGGTCAGCGCCGATGCGCTGGAAAAGCTGAAGAGCTACCG
TTTCCCCGGCAACGTACGCGAACTGGAGAACATGCTCGAGCGCGCCTACACGCTGTGCGAGGACGACCTCATCCAGCCGC
ACGACCTGCGCTTCGCGGAAACCGCGAGTGGCGGCGAAAGCGGCGAAGCCAGCCTGGCGCAGATCGACAACCTCGAAGAC
TACCTGGAAGAGATCGAACGCAAGCTGATCATGCAGGCACTCGAGGAAACCCGCTGGAACCGCACTGCGGCCGCCCAGCG
TCTGGGCCTGACCTTCCGCTCCATGCGCTACCGGCTGAAGAAGCTCGGTATCGATTGA

Domains

Predicted by InterproScan.

• Bacterial regulatory protein, Fis family (HTH_8) (402-442)

• Sigma-54 interaction domain (Sigma54_activat) (135-301)

• Response regulator receiver domain (Response_reg) (7-115)

3D structure

• AlphaFold DB: A0A024HNN1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	87.64	100	0.876
pilR	Acinetobacter baumannii strain A118	50.109	100	0.517