Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACOB85_RS09225 Genome accession   NZ_CP184834
Coordinates   319860..320876 (+) Length   338 a.a.
NCBI ID   WP_005381324.1    Uniprot ID   A0AAW9BNQ7
Organism   Vibrio alginolyticus strain HoVa25     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 314860..325876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOB85_RS09210 (ACOB85_09210) - 315291..316019 (-) 729 WP_005382781.1 redoxin family protein -
  ACOB85_RS09215 (ACOB85_09215) oxyR 316189..317100 (+) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  ACOB85_RS09220 (ACOB85_09220) - 317154..319715 (-) 2562 WP_005381325.1 penicillin-binding protein 1A -
  ACOB85_RS09225 (ACOB85_09225) pilM 319860..320876 (+) 1017 WP_005381324.1 type IV pilus biogenesis protein PilM Machinery gene
  ACOB85_RS09230 (ACOB85_09230) pilN 320860..321435 (+) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  ACOB85_RS09235 (ACOB85_09235) pilO 321428..322021 (+) 594 WP_005382767.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACOB85_RS09240 (ACOB85_09240) pilP 322011..322526 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  ACOB85_RS09245 (ACOB85_09245) pilQ 322552..324297 (+) 1746 WP_262580159.1 type IV pilus secretin PilQ Machinery gene
  ACOB85_RS09250 (ACOB85_09250) aroK 324485..325003 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37581.92 Da        Isoelectric Point: 7.2224

>NTDB_id=1111365 ACOB85_RS09225 WP_005381324.1 319860..320876(+) (pilM) [Vibrio alginolyticus strain HoVa25]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGANSASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1111365 ACOB85_RS09225 WP_005381324.1 319860..320876(+) (pilM) [Vibrio alginolyticus strain HoVa25]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
CTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGTGCTAAAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACTTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGCAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

83.136

100

0.831
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503

Multiple sequence alignment