Detailed information NTG1109625

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACOCJA_RS02340
• Genome accession: NZ_CP184565
• Coordinates: 434665..435162 (+)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain CBTW2018043
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 429665..440162

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOCJA_RS02300 (ACOCJA_02300)	-	429709..430695 (+)	987	WP_000161315.1	ROK family glucokinase	-
ACOCJA_RS02305 (ACOCJA_02305)	-	430695..431024 (+)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACOCJA_RS02310 (ACOCJA_02310)	-	431021..431644 (+)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACOCJA_RS02315 (ACOCJA_02315)	comGA	431696..432670 (+)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACOCJA_RS02320 (ACOCJA_02320)	comGB	432642..433712 (+)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACOCJA_RS02325 (ACOCJA_02325)	comGC	433726..434037 (+)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ACOCJA_RS02330 (ACOCJA_02330)	comGD	434015..434461 (+)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACOCJA_RS02335 (ACOCJA_02335)	comGE	434448..434747 (+)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACOCJA_RS02340 (ACOCJA_02340)	comGF	434665..435162 (+)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACOCJA_RS02345 (ACOCJA_02345)	-	435259..435405 (+)	147	WP_001789879.1	hypothetical protein	-
ACOCJA_RS02350 (ACOCJA_02350)	-	435395..435919 (+)	525	WP_001015120.1	shikimate kinase	-
ACOCJA_RS02355 (ACOCJA_02355)	gcvT	436078..437169 (+)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACOCJA_RS02360 (ACOCJA_02360)	gcvPA	437189..438535 (+)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACOCJA_RS02365 (ACOCJA_02365)	gcvPB	438528..440000 (+)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=1109625 ACOCJA_RS02340 WP_001825340.1 434665..435162(+) (comGF) [Staphylococcus aureus strain CBTW2018043]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1109625 ACOCJA_RS02340 WP_001825340.1 434665..435162(+) (comGF) [Staphylococcus aureus strain CBTW2018043]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97