Detailed information NTG1109591

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACOCI8_RS08345
• Genome accession: NZ_CP184563
• Coordinates: 1659802..1660299 (+)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain SCTW2018065
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1654802..1665299

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOCI8_RS08305 (ACOCI8_08305)	-	1654846..1655832 (+)	987	WP_000161315.1	ROK family glucokinase	-
ACOCI8_RS08310 (ACOCI8_08310)	-	1655832..1656161 (+)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACOCI8_RS08315 (ACOCI8_08315)	-	1656158..1656781 (+)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACOCI8_RS08320 (ACOCI8_08320)	comGA	1656833..1657807 (+)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACOCI8_RS08325 (ACOCI8_08325)	comGB	1657779..1658849 (+)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACOCI8_RS08330 (ACOCI8_08330)	comGC	1658863..1659174 (+)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ACOCI8_RS08335 (ACOCI8_08335)	comGD	1659152..1659598 (+)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACOCI8_RS08340 (ACOCI8_08340)	comGE	1659585..1659884 (+)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACOCI8_RS08345 (ACOCI8_08345)	comGF	1659802..1660299 (+)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACOCI8_RS08350 (ACOCI8_08350)	-	1660396..1660542 (+)	147	WP_001789879.1	hypothetical protein	-
ACOCI8_RS08355 (ACOCI8_08355)	-	1660532..1661056 (+)	525	WP_001015120.1	shikimate kinase	-
ACOCI8_RS08360 (ACOCI8_08360)	gcvT	1661215..1662306 (+)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACOCI8_RS08365 (ACOCI8_08365)	gcvPA	1662326..1663672 (+)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACOCI8_RS08370 (ACOCI8_08370)	gcvPB	1663665..1665137 (+)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=1109591 ACOCI8_RS08345 WP_001825340.1 1659802..1660299(+) (comGF) [Staphylococcus aureus strain SCTW2018065]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1109591 ACOCI8_RS08345 WP_001825340.1 1659802..1660299(+) (comGF) [Staphylococcus aureus strain SCTW2018065]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97