Detailed information NTG1109538

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACOCJJ_RS07640
• Genome accession: NZ_CP184561
• Coordinates: 1592579..1593076 (-)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain CBTW2018311
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1587579..1598076

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOCJJ_RS07615 (ACOCJJ_07615)	gcvPB	1587741..1589213 (-)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
ACOCJJ_RS07620 (ACOCJJ_07620)	gcvPA	1589206..1590552 (-)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACOCJJ_RS07625 (ACOCJJ_07625)	gcvT	1590572..1591663 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACOCJJ_RS07630 (ACOCJJ_07630)	-	1591822..1592346 (-)	525	WP_001015120.1	shikimate kinase	-
ACOCJJ_RS07635 (ACOCJJ_07635)	-	1592336..1592482 (-)	147	WP_001789879.1	hypothetical protein	-
ACOCJJ_RS07640 (ACOCJJ_07640)	comGF	1592579..1593076 (-)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACOCJJ_RS07645 (ACOCJJ_07645)	comGE	1592994..1593293 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACOCJJ_RS07650 (ACOCJJ_07650)	comGD	1593280..1593726 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACOCJJ_RS07655 (ACOCJJ_07655)	comGC	1593704..1594015 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ACOCJJ_RS07660 (ACOCJJ_07660)	comGB	1594029..1595099 (-)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACOCJJ_RS07665 (ACOCJJ_07665)	comGA	1595071..1596045 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACOCJJ_RS07670 (ACOCJJ_07670)	-	1596097..1596720 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACOCJJ_RS07675 (ACOCJJ_07675)	-	1596717..1597046 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACOCJJ_RS07680 (ACOCJJ_07680)	-	1597046..1598032 (-)	987	WP_000161315.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=1109538 ACOCJJ_RS07640 WP_001825340.1 1592579..1593076(-) (comGF) [Staphylococcus aureus strain CBTW2018311]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1109538 ACOCJJ_RS07640 WP_001825340.1 1592579..1593076(-) (comGF) [Staphylococcus aureus strain CBTW2018311]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97