Detailed information NTG1109449

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACOCJB_RS02745
• Genome accession: NZ_CP184557
• Coordinates: 503144..503641 (+)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain SCTW2018482
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 498144..508641

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOCJB_RS02705 (ACOCJB_02705)	-	498188..499174 (+)	987	WP_000161315.1	ROK family glucokinase	-
ACOCJB_RS02710 (ACOCJB_02710)	-	499174..499503 (+)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACOCJB_RS02715 (ACOCJB_02715)	-	499500..500123 (+)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACOCJB_RS02720 (ACOCJB_02720)	comGA	500175..501149 (+)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACOCJB_RS02725 (ACOCJB_02725)	comGB	501121..502191 (+)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACOCJB_RS02730 (ACOCJB_02730)	comGC	502205..502516 (+)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ACOCJB_RS02735 (ACOCJB_02735)	comGD	502494..502940 (+)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACOCJB_RS02740 (ACOCJB_02740)	comGE	502927..503226 (+)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACOCJB_RS02745 (ACOCJB_02745)	comGF	503144..503641 (+)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACOCJB_RS02750 (ACOCJB_02750)	-	503738..503884 (+)	147	WP_001789879.1	hypothetical protein	-
ACOCJB_RS02755 (ACOCJB_02755)	-	503874..504398 (+)	525	WP_001015120.1	shikimate kinase	-
ACOCJB_RS02760 (ACOCJB_02760)	gcvT	504557..505648 (+)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACOCJB_RS02765 (ACOCJB_02765)	gcvPA	505668..507014 (+)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACOCJB_RS02770 (ACOCJB_02770)	gcvPB	507007..508479 (+)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=1109449 ACOCJB_RS02745 WP_001825340.1 503144..503641(+) (comGF) [Staphylococcus aureus strain SCTW2018482]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1109449 ACOCJB_RS02745 WP_001825340.1 503144..503641(+) (comGF) [Staphylococcus aureus strain SCTW2018482]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97