Detailed information NTG1109363

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACOCJI_RS10145
• Genome accession: NZ_CP184551
• Coordinates: 2119696..2120193 (-)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain SCTW2019099
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2114696..2125193

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOCJI_RS10120 (ACOCJI_10120)	gcvPB	2114858..2116330 (-)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
ACOCJI_RS10125 (ACOCJI_10125)	gcvPA	2116323..2117669 (-)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACOCJI_RS10130 (ACOCJI_10130)	gcvT	2117689..2118780 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACOCJI_RS10135 (ACOCJI_10135)	-	2118939..2119463 (-)	525	WP_001015120.1	shikimate kinase	-
ACOCJI_RS10140 (ACOCJI_10140)	-	2119453..2119599 (-)	147	WP_001789879.1	hypothetical protein	-
ACOCJI_RS10145 (ACOCJI_10145)	comGF	2119696..2120193 (-)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACOCJI_RS10150 (ACOCJI_10150)	comGE	2120111..2120410 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACOCJI_RS10155 (ACOCJI_10155)	comGD	2120397..2120843 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACOCJI_RS10160 (ACOCJI_10160)	comGC	2120821..2121132 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ACOCJI_RS10165 (ACOCJI_10165)	comGB	2121146..2122216 (-)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACOCJI_RS10170 (ACOCJI_10170)	comGA	2122188..2123162 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACOCJI_RS10175 (ACOCJI_10175)	-	2123214..2123837 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACOCJI_RS10180 (ACOCJI_10180)	-	2123834..2124163 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACOCJI_RS10185 (ACOCJI_10185)	-	2124163..2125149 (-)	987	WP_000161315.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=1109363 ACOCJI_RS10145 WP_001825340.1 2119696..2120193(-) (comGF) [Staphylococcus aureus strain SCTW2019099]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1109363 ACOCJI_RS10145 WP_001825340.1 2119696..2120193(-) (comGF) [Staphylococcus aureus strain SCTW2019099]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97