Detailed information NTG1109325

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACOCJC_RS08790
• Genome accession: NZ_CP184549
• Coordinates: 1740990..1741487 (+)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain SCTW2019145
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1735990..1746487

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOCJC_RS08750 (ACOCJC_08750)	-	1736034..1737020 (+)	987	WP_000161315.1	ROK family glucokinase	-
ACOCJC_RS08755 (ACOCJC_08755)	-	1737020..1737349 (+)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACOCJC_RS08760 (ACOCJC_08760)	-	1737346..1737969 (+)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACOCJC_RS08765 (ACOCJC_08765)	comGA	1738021..1738995 (+)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACOCJC_RS08770 (ACOCJC_08770)	comGB	1738967..1740037 (+)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACOCJC_RS08775 (ACOCJC_08775)	comGC	1740051..1740362 (+)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ACOCJC_RS08780 (ACOCJC_08780)	comGD	1740340..1740786 (+)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACOCJC_RS08785 (ACOCJC_08785)	comGE	1740773..1741072 (+)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACOCJC_RS08790 (ACOCJC_08790)	comGF	1740990..1741487 (+)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACOCJC_RS08795 (ACOCJC_08795)	-	1741584..1741730 (+)	147	WP_001789879.1	hypothetical protein	-
ACOCJC_RS08800 (ACOCJC_08800)	-	1741720..1742244 (+)	525	WP_001015120.1	shikimate kinase	-
ACOCJC_RS08805 (ACOCJC_08805)	gcvT	1742403..1743494 (+)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACOCJC_RS08810 (ACOCJC_08810)	gcvPA	1743514..1744860 (+)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACOCJC_RS08815 (ACOCJC_08815)	gcvPB	1744853..1746325 (+)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=1109325 ACOCJC_RS08790 WP_001825340.1 1740990..1741487(+) (comGF) [Staphylococcus aureus strain SCTW2019145]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1109325 ACOCJC_RS08790 WP_001825340.1 1740990..1741487(+) (comGF) [Staphylococcus aureus strain SCTW2019145]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97