Detailed information NTG1109271

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACOCJE_RS02305
• Genome accession: NZ_CP184547
• Coordinates: 427644..428141 (+)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain SCTW2021015
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 422644..433141

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOCJE_RS02265 (ACOCJE_02265)	-	422688..423674 (+)	987	WP_420228466.1	ROK family glucokinase	-
ACOCJE_RS02270 (ACOCJE_02270)	-	423674..424003 (+)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACOCJE_RS02275 (ACOCJE_02275)	-	424000..424623 (+)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACOCJE_RS02280 (ACOCJE_02280)	comGA	424675..425649 (+)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACOCJE_RS02285 (ACOCJE_02285)	comGB	425621..426691 (+)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACOCJE_RS02290 (ACOCJE_02290)	comGC	426705..427016 (+)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ACOCJE_RS02295 (ACOCJE_02295)	comGD	426994..427440 (+)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACOCJE_RS02300 (ACOCJE_02300)	comGE	427427..427726 (+)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACOCJE_RS02305 (ACOCJE_02305)	comGF	427644..428141 (+)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACOCJE_RS02310 (ACOCJE_02310)	-	428238..428384 (+)	147	WP_001789879.1	hypothetical protein	-
ACOCJE_RS02315 (ACOCJE_02315)	-	428374..428898 (+)	525	WP_001015120.1	shikimate kinase	-
ACOCJE_RS02320 (ACOCJE_02320)	gcvT	429057..430148 (+)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACOCJE_RS02325 (ACOCJE_02325)	gcvPA	430168..431514 (+)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACOCJE_RS02330 (ACOCJE_02330)	gcvPB	431507..432979 (+)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=1109271 ACOCJE_RS02305 WP_001825340.1 427644..428141(+) (comGF) [Staphylococcus aureus strain SCTW2021015]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1109271 ACOCJE_RS02305 WP_001825340.1 427644..428141(+) (comGF) [Staphylococcus aureus strain SCTW2021015]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97