Detailed information NTG1109182

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACOCI7_RS02335
• Genome accession: NZ_CP184543
• Coordinates: 434447..434944 (+)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain CBTW2022289
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 429447..439944

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOCI7_RS02295 (ACOCI7_02295)	-	429491..430477 (+)	987	WP_000161315.1	ROK family glucokinase	-
ACOCI7_RS02300 (ACOCI7_02300)	-	430477..430806 (+)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACOCI7_RS02305 (ACOCI7_02305)	-	430803..431426 (+)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACOCI7_RS02310 (ACOCI7_02310)	comGA	431478..432452 (+)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACOCI7_RS02315 (ACOCI7_02315)	comGB	432424..433494 (+)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACOCI7_RS02320 (ACOCI7_02320)	comGC	433508..433819 (+)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ACOCI7_RS02325 (ACOCI7_02325)	comGD	433797..434243 (+)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACOCI7_RS02330 (ACOCI7_02330)	comGE	434230..434529 (+)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACOCI7_RS02335 (ACOCI7_02335)	comGF	434447..434944 (+)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACOCI7_RS02340 (ACOCI7_02340)	-	435041..435187 (+)	147	WP_001789879.1	hypothetical protein	-
ACOCI7_RS02345 (ACOCI7_02345)	-	435177..435701 (+)	525	WP_001015120.1	shikimate kinase	-
ACOCI7_RS02350 (ACOCI7_02350)	gcvT	435860..436951 (+)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACOCI7_RS02355 (ACOCI7_02355)	gcvPA	436971..438317 (+)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACOCI7_RS02360 (ACOCI7_02360)	gcvPB	438310..439782 (+)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=1109182 ACOCI7_RS02335 WP_001825340.1 434447..434944(+) (comGF) [Staphylococcus aureus strain CBTW2022289]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1109182 ACOCI7_RS02335 WP_001825340.1 434447..434944(+) (comGF) [Staphylococcus aureus strain CBTW2022289]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97