Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACNBEW_RS12140 Genome accession   NZ_CP184280
Coordinates   2518398..2519012 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain GHZJ     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2513398..2524012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNBEW_RS12110 (ACNBEW_12110) rpsT 2513701..2513967 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  ACNBEW_RS12115 (ACNBEW_12115) holA 2513984..2515025 (-) 1042 Protein_2338 DNA polymerase III subunit delta -
  ACNBEW_RS12120 (ACNBEW_12120) - 2515065..2515216 (-) 152 Protein_2339 hypothetical protein -
  ACNBEW_RS12125 (ACNBEW_12125) - 2515257..2515391 (+) 135 WP_003152870.1 YqzM family protein -
  ACNBEW_RS12130 (ACNBEW_12130) comEC 2515410..2517761 (-) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACNBEW_RS12135 (ACNBEW_12135) - 2517762..2518331 (-) 570 WP_003152868.1 ComE operon protein 2 -
  ACNBEW_RS12140 (ACNBEW_12140) comEA 2518398..2519012 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  ACNBEW_RS12145 (ACNBEW_12145) comER 2519071..2519892 (+) 822 WP_012118027.1 late competence protein ComER -
  ACNBEW_RS12150 (ACNBEW_12150) - 2519961..2520698 (-) 738 WP_032873951.1 class I SAM-dependent DNA methyltransferase -
  ACNBEW_RS12155 (ACNBEW_12155) rsfS 2520695..2521051 (-) 357 WP_007408260.1 ribosome silencing factor -
  ACNBEW_RS12160 (ACNBEW_12160) yqeK 2521055..2521630 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACNBEW_RS12165 (ACNBEW_12165) - 2521620..2522189 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  ACNBEW_RS12170 (ACNBEW_12170) yhbY 2522200..2522490 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  ACNBEW_RS12175 (ACNBEW_12175) aroE 2522484..2523326 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=1108189 ACNBEW_RS12140 WP_032873955.1 2518398..2519012(-) (comEA) [Bacillus velezensis strain GHZJ]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1108189 ACNBEW_RS12140 WP_032873955.1 2518398..2519012(-) (comEA) [Bacillus velezensis strain GHZJ]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363

Multiple sequence alignment