Detailed information NTG1108187

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: ACNBEW_RS12130
• Genome accession: NZ_CP184280
• Coordinates: 2515410..2517761 (-)
• Length: 783 a.a.
• NCBI ID: WP_032873958.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain GHZJ
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2510410..2522761

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACNBEW_RS12095 (ACNBEW_12095)	-	2510776..2511114 (-)	339	WP_007408270.1	YqxA family protein	-
ACNBEW_RS12100 (ACNBEW_12100)	spoIIP	2511131..2512324 (-)	1194	WP_032873961.1	stage II sporulation protein P	-
ACNBEW_RS12105 (ACNBEW_12105)	gpr	2512392..2513498 (-)	1107	WP_007408268.1	GPR endopeptidase	-
ACNBEW_RS12110 (ACNBEW_12110)	rpsT	2513701..2513967 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACNBEW_RS12115 (ACNBEW_12115)	holA	2513984..2515025 (-)	1042	Protein_2338	DNA polymerase III subunit delta	-
ACNBEW_RS12120 (ACNBEW_12120)	-	2515065..2515216 (-)	152	Protein_2339	hypothetical protein	-
ACNBEW_RS12125 (ACNBEW_12125)	-	2515257..2515391 (+)	135	WP_003152870.1	YqzM family protein	-
ACNBEW_RS12130 (ACNBEW_12130)	comEC	2515410..2517761 (-)	2352	WP_032873958.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACNBEW_RS12135 (ACNBEW_12135)	-	2517762..2518331 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACNBEW_RS12140 (ACNBEW_12140)	comEA	2518398..2519012 (-)	615	WP_032873955.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACNBEW_RS12145 (ACNBEW_12145)	comER	2519071..2519892 (+)	822	WP_012118027.1	late competence protein ComER	-
ACNBEW_RS12150 (ACNBEW_12150)	-	2519961..2520698 (-)	738	WP_032873951.1	class I SAM-dependent DNA methyltransferase	-
ACNBEW_RS12155 (ACNBEW_12155)	rsfS	2520695..2521051 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACNBEW_RS12160 (ACNBEW_12160)	yqeK	2521055..2521630 (-)	576	WP_032873949.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACNBEW_RS12165 (ACNBEW_12165)	-	2521620..2522189 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACNBEW_RS12170 (ACNBEW_12170)	yhbY	2522200..2522490 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86681.60 Da
• Isoelectric Point: 9.1106

>NTDB_id=1108187 ACNBEW_RS12130 WP_032873958.1 2515410..2517761(-) (comEC) [Bacillus velezensis strain GHZJ]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKHHAPVIVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLHSQQI
HWLFSVTSIQQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGEKFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLMFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFLMNMVLVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFVSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVISYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKVYSVNVLRTDVSGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=1108187 ACNBEW_RS12130 WP_032873958.1 2515410..2517761(-) (comEC) [Bacillus velezensis strain GHZJ]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCACCATGCTCCGGTTATCGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGTTCTCTTGAGGAAAAAAGACTTATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTCACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAAAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCGGGAATTTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGATGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCGGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCTGATGAATATGGTGTTGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTTATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTCGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGATTTTGTTTCCC
TTCTTCTGCTTGCGGTTTCAGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTTCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGAGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAAGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCTACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGTGTACTCTGTCAATGTGCTTCGCACCGATGT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

• Competence protein (Competence) (208-469)

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.606	98.595	0.558