Detailed information NTG1108125

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACOIYJ_RS12640
• Genome accession: NZ_CP184271
• Coordinates: 2773622..2774461 (+)
• Length: 279 a.a.
• NCBI ID: WP_180741896.1
• Uniprot ID: A0A7V8TCD9
• Organism: Pectobacterium brasiliense strain CSR2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2768622..2779461

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOIYJ_RS12610	gspI	2769014..2769379 (+)	366	WP_180741890.1	type II secretion system minor pseudopilin GspI	-
ACOIYJ_RS12615	gspJ	2769379..2770029 (+)	651	WP_420064276.1	type II secretion system minor pseudopilin GspJ	-
ACOIYJ_RS12620	gspK	2770026..2771012 (+)	987	WP_420064275.1	type II secretion system minor pseudopilin GspK	-
ACOIYJ_RS12625	gspL	2771009..2772286 (+)	1278	WP_420064274.1	type II secretion system protein GspL	-
ACOIYJ_RS12630	gspM	2772283..2772777 (+)	495	WP_420064273.1	type II secretion system protein GspM	-
ACOIYJ_RS12635	-	2772767..2773513 (+)	747	WP_420064272.1	type II secretion system protein N	-
ACOIYJ_RS12640	pilD	2773622..2774461 (+)	840	WP_180741896.1	prepilin peptidase	Machinery gene
ACOIYJ_RS12645	ypdK	2774604..2774678 (-)	75	WP_099606229.1	membrane protein YpdK	-
ACOIYJ_RS12650	-	2775034..2776059 (-)	1026	WP_180741897.1	LLM class flavin-dependent oxidoreductase	-
ACOIYJ_RS12655	-	2776448..2776930 (+)	483	Protein_2492	ABC transporter substrate-binding protein	-
ACOIYJ_RS12660	-	2776957..2777124 (+)	168	Protein_2493	ABC transporter ATP-binding protein	-
ACOIYJ_RS12665	-	2777121..2777855 (+)	735	WP_420064271.1	molybdate ABC transporter substrate-binding protein	-
ACOIYJ_RS12670	dbpA	2777977..2779359 (+)	1383	WP_180741899.1	ATP-dependent RNA helicase DbpA	-

Sequence

Protein

• Length: 279 a.a.
• Molecular weight: 31146.08 Da
• Isoelectric Point: 7.5535

>NTDB_id=1108125 ACOIYJ_RS12640 WP_180741896.1 2773622..2774461(+) (pilD) [Pectobacterium brasiliense strain CSR2]
MDDLREFAQVFPAWWYGALGVLGLIVGSFLNVVIYRLPIMLERRWRQDIDLQTGVTDAEQATRYNLCWPPSSCPHCQQSI
AVKDNIPLFSWLWLRGRSRCCHQPVSVQYPLVEVITMLAFLAAGLLWLPSMALCAALILLSFLVVLTVIDIKTLLLPDVL
TLSLLWIGLLFNLSETFVPLRDAVVGAMAGYLSLWLLYWVFKYTTGKDALGYGDFKLLSALGAWLGWQALPNLVLVAALS
GLVMTLIWRGLRKEDTTKPLAFGPWLAVGGVFSVIMNGL

Nucleotide

• Length: 840 bp

>NTDB_id=1108125 ACOIYJ_RS12640 WP_180741896.1 2773622..2774461(+) (pilD) [Pectobacterium brasiliense strain CSR2]
GTGGACGATTTAAGGGAATTTGCGCAGGTGTTTCCAGCATGGTGGTATGGTGCGCTGGGCGTATTGGGATTGATTGTTGG
CAGCTTTCTGAACGTAGTGATTTATCGTTTGCCGATTATGTTGGAACGGCGCTGGCGGCAAGACATCGATCTGCAAACCG
GCGTAACGGATGCCGAGCAGGCTACGCGCTATAACCTGTGCTGGCCGCCTTCGTCTTGCCCACACTGCCAGCAGAGTATT
GCGGTGAAGGATAACATTCCGTTATTCAGTTGGCTGTGGCTGCGCGGTCGTTCCCGATGCTGTCATCAACCGGTGTCTGT
GCAGTATCCGCTGGTGGAAGTCATCACCATGCTGGCTTTTCTGGCTGCGGGGCTGCTGTGGTTGCCCAGTATGGCGCTGT
GTGCGGCGTTAATCCTGCTGTCCTTTCTGGTGGTGCTGACCGTCATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTCTTGTGGATAGGGTTGCTGTTTAATCTATCGGAAACGTTTGTCCCATTACGTGATGCCGTAGTAGGGGC
GATGGCGGGCTATTTGTCCCTGTGGTTACTTTATTGGGTATTTAAATATACCACTGGCAAAGACGCGCTGGGATACGGTG
ATTTTAAGTTACTGTCTGCGTTGGGAGCCTGGCTAGGCTGGCAGGCATTGCCGAATCTTGTTTTGGTGGCGGCGCTGAGC
GGTCTGGTCATGACGCTTATTTGGCGTGGATTGCGTAAAGAAGATACAACTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
TGTCGGTGGTGTATTCAGTGTGATAATGAACGGGCTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

3D structure

• AlphaFold DB: A0A7V8TCD9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.815	97.133	0.484
pilD	Neisseria gonorrhoeae MS11	48.617	90.681	0.441
pilD	Vibrio campbellii strain DS40M4	44.161	98.208	0.434
pilD	Acinetobacter baumannii D1279779	42.857	92.832	0.398
pilD	Acinetobacter nosocomialis M2	42.857	92.832	0.398