Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   ACN9KP_RS12065 Genome accession   NZ_CP183947
Coordinates   2489856..2490893 (-) Length   345 a.a.
NCBI ID   WP_024085602.1    Uniprot ID   -
Organism   Bacillus velezensis strain Ya-1 isolate Tropical rainforest soil     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2484856..2495893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN9KP_RS12015 (ACN9KP_12015) tasA 2485090..2485875 (-) 786 WP_003153102.1 biofilm matrix protein TasA -
  ACN9KP_RS12020 (ACN9KP_12020) sipW 2485939..2486523 (-) 585 WP_012117977.1 signal peptidase I SipW -
  ACN9KP_RS12025 (ACN9KP_12025) tapA 2486495..2487166 (-) 672 WP_024085598.1 amyloid fiber anchoring/assembly protein TapA -
  ACN9KP_RS12030 (ACN9KP_12030) - 2487426..2487755 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  ACN9KP_RS12035 (ACN9KP_12035) - 2487795..2487974 (-) 180 WP_003153093.1 YqzE family protein -
  ACN9KP_RS12040 (ACN9KP_12040) comGG 2488031..2488408 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACN9KP_RS12045 (ACN9KP_12045) comGF 2488409..2488804 (-) 396 WP_283002417.1 competence type IV pilus minor pilin ComGF -
  ACN9KP_RS12050 (ACN9KP_12050) comGE 2488818..2489132 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACN9KP_RS12055 (ACN9KP_12055) comGD 2489116..2489553 (-) 438 WP_044053464.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACN9KP_RS12060 (ACN9KP_12060) comGC 2489543..2489851 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  ACN9KP_RS12065 (ACN9KP_12065) comGB 2489856..2490893 (-) 1038 WP_024085602.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACN9KP_RS12070 (ACN9KP_12070) comGA 2490880..2491950 (-) 1071 WP_044053465.1 competence type IV pilus ATPase ComGA Machinery gene
  ACN9KP_RS12075 (ACN9KP_12075) - 2492142..2493092 (-) 951 WP_014305415.1 magnesium transporter CorA family protein -
  ACN9KP_RS12080 (ACN9KP_12080) - 2493238..2494539 (+) 1302 WP_014305416.1 hemolysin family protein -
  ACN9KP_RS12085 (ACN9KP_12085) - 2494577..2495407 (-) 831 WP_044053466.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39618.91 Da        Isoelectric Point: 9.5658

>NTDB_id=1107320 ACN9KP_RS12065 WP_024085602.1 2489856..2490893(-) (comGB) [Bacillus velezensis strain Ya-1 isolate Tropical rainforest soil]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYEVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPYYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1107320 ACN9KP_RS12065 WP_024085602.1 2489856..2490893(-) (comGB) [Bacillus velezensis strain Ya-1 isolate Tropical rainforest soil]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGCTGAGGGAAGGTGATGCGTTTTACGAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCAGAGAAACACGGAGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTCATTGTTGTGAAAATACCCCTCTTGGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTATTATGAAAAAGATCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCTAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614

Multiple sequence alignment