Detailed information NTG1107093

Bioinformatically predicted

Overview

• Name: comE
• Type: Machinery gene
• Locus tag: ACLKM1_RS11750
• Genome accession: NZ_CP183903
• Coordinates: 2445627..2446085 (-)
• Length: 152 a.a.
• NCBI ID: WP_411689804.1
• Uniprot ID: -
• Organism: Acinetobacter pseudolwoffii strain M5_NDM
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2445627..2464767	2445627..2446085	within	0

Gene organization within MGE regions

Location: 2445627..2464767

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLKM1_RS11750	comE	2445627..2446085 (-)	459	WP_411689804.1	type IV pilin protein	Machinery gene
ACLKM1_RS11755	-	2446095..2450039 (-)	3945	WP_411689802.1	pilus assembly protein PilY	-
ACLKM1_RS11760	-	2450055..2450822 (-)	768	WP_411689801.1	pilus assembly protein PilX	-
ACLKM1_RS11765	-	2450819..2451859 (-)	1041	WP_411689800.1	PilW family protein	-
ACLKM1_RS11770	pilV	2451856..2452377 (-)	522	WP_411689798.1	type IV pilus modification protein PilV	-
ACLKM1_RS11775	-	2452377..2452814 (-)	438	WP_411689797.1	pilus assembly FimT family protein	-
ACLKM1_RS11780	ispH	2452976..2453926 (-)	951	WP_005168840.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
ACLKM1_RS11785	gmk	2454061..2454684 (+)	624	WP_005094292.1	guanylate kinase	-
ACLKM1_RS11790	rpoZ	2454761..2455042 (+)	282	WP_004650579.1	DNA-directed RNA polymerase subunit omega	-
ACLKM1_RS11795	-	2455257..2457362 (+)	2106	WP_180049912.1	RelA/SpoT family protein	-
ACLKM1_RS11800	-	2457436..2458494 (-)	1059	WP_004787095.1	RNA-guided endonuclease InsQ/TnpB family protein	-
ACLKM1_RS11805	-	2458626..2459009 (+)	384	WP_411689796.1	RidA family protein	-
ACLKM1_RS11810	-	2459228..2459422 (+)	195	WP_004732422.1	bacterioferritin-associated ferredoxin	-
ACLKM1_RS11815	bfr	2459646..2460110 (+)	465	WP_180075329.1	heteropolymeric bacterioferritin subunit Bfr	-
ACLKM1_RS11820	-	2460526..2462160 (-)	1635	WP_411689795.1	PglL family O-oligosaccharyltransferase	-
ACLKM1_RS11825	-	2462308..2463240 (+)	933	Protein_2272	IS5-like element IS17 family transposase	-
ACLKM1_RS11830	-	2463301..2464767 (-)	1467	WP_411689816.1	O-antigen ligase family protein	-

Sequence

Protein

• Length: 152 a.a.
• Molecular weight: 17111.63 Da
• Isoelectric Point: 9.8035

>NTDB_id=1107093 ACLKM1_RS11750 WP_411689804.1 2445627..2446085(-) (comE) [Acinetobacter pseudolwoffii strain M5_NDM]
MKYRNFPKGFTLLELMVVVVIIAVFAAIAFPSYQLYVRRAMAAQAQDRVQYIASELERYKSRNFNYMNFTIPLDQRTIPS
GATGSDIKYSISMLADANGQNWILKAESKDNRNDSFLMTSLGQRCKTKHWSNINSIAITGIANAHCGGGESW

Nucleotide

• Length: 459 bp

>NTDB_id=1107093 ACLKM1_RS11750 WP_411689804.1 2445627..2446085(-) (comE) [Acinetobacter pseudolwoffii strain M5_NDM]
ATGAAGTATAGAAATTTCCCAAAGGGATTCACGTTGCTTGAATTAATGGTTGTTGTTGTCATTATTGCTGTATTCGCTGC
TATAGCTTTTCCTAGCTATCAATTGTATGTTAGGCGGGCAATGGCGGCTCAGGCTCAGGATCGGGTTCAATATATTGCAT
CTGAGCTTGAGCGTTATAAGTCACGTAATTTTAATTATATGAACTTTACTATACCTTTGGATCAACGAACTATTCCGTCA
GGAGCAACGGGTAGTGATATTAAATATAGTATCTCAATGCTAGCAGATGCTAATGGACAAAATTGGATACTTAAGGCTGA
AAGTAAAGACAATAGAAATGATAGTTTTCTAATGACAAGCTTAGGCCAGAGGTGTAAAACCAAACATTGGAGTAATATTA
ATTCTATTGCTATTACAGGGATTGCAAACGCTCATTGTGGTGGAGGAGAATCATGGTAA

Domains

Predicted by InterproScan.

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (31-113)

• Prokaryotic N-terminal methylation motif (N_methyl) (8-30)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Acinetobacter baylyi ADP1	43.023	100	0.487
pilY2	Acinetobacter baumannii D1279779	41.892	97.368	0.408