Detailed information NTG1106986

Bioinformatically predicted

Overview

• Name: comE
• Type: Machinery gene
• Locus tag: ACLKM4_RS12670
• Genome accession: NZ_CP183895
• Coordinates: 2640665..2641126 (-)
• Length: 153 a.a.
• NCBI ID: WP_286396274.1
• Uniprot ID: -
• Organism: Acinetobacter indicus strain D1_NDM_tet(X3)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2640665..2657553	2640665..2641126	within	0

Gene organization within MGE regions

Location: 2640665..2657553

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLKM4_RS12670	comE	2640665..2641126 (-)	462	WP_286396274.1	type IV pilin protein	Machinery gene
ACLKM4_RS12675	-	2641136..2645137 (-)	4002	WP_411687474.1	pilus assembly protein PilY	-
ACLKM4_RS12680	-	2645156..2645917 (-)	762	WP_180002716.1	pilus assembly PilX family protein	-
ACLKM4_RS12685	-	2645914..2646927 (-)	1014	WP_411687473.1	PilW family protein	-
ACLKM4_RS12690	pilV	2646930..2647436 (-)	507	WP_411687472.1	type IV pilus modification protein PilV	-
ACLKM4_RS12695	-	2647436..2647924 (-)	489	WP_411687471.1	GspH/FimT family pseudopilin	-
ACLKM4_RS12700	ispH	2648088..2649038 (-)	951	WP_411687494.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
ACLKM4_RS12705	gmk	2649174..2649797 (+)	624	WP_045795273.1	guanylate kinase	-
ACLKM4_RS12710	rpoZ	2649874..2650155 (+)	282	WP_004809957.1	DNA-directed RNA polymerase subunit omega	-
ACLKM4_RS12715	-	2650378..2652477 (+)	2100	WP_160240914.1	RelA/SpoT family protein	-
ACLKM4_RS12720	-	2652549..2652932 (+)	384	WP_104513240.1	RidA family protein	-
ACLKM4_RS12725	-	2653087..2653281 (+)	195	WP_034597991.1	bacterioferritin-associated ferredoxin	-
ACLKM4_RS12730	bfr	2653508..2653972 (+)	465	WP_411687470.1	heteropolymeric bacterioferritin subunit Bfr	-
ACLKM4_RS12735	-	2654084..2655724 (-)	1641	WP_411687469.1	PglL family O-oligosaccharyltransferase	-
ACLKM4_RS12740	-	2655946..2657553 (-)	1608	WP_411687468.1	PglL family O-oligosaccharyltransferase	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17200.53 Da
• Isoelectric Point: 9.3215

>NTDB_id=1106986 ACLKM4_RS12670 WP_286396274.1 2640665..2641126(-) (comE) [Acinetobacter indicus strain D1_NDM_tet(X3)]
MNKMNKFAERGFTLIELMVVVVIVAIFAAIAIPSYQNYVRRAEASQVQQEIQRLASELSKWKSRNFSYQGFNLTPQTIRN
YTIEIKDGTDTAFKLDDKDGTEYKANGQQWVIKAIHADGTNVSFLQTSTGLKCKNKAKANITYSSCGTGGEEW

Nucleotide

• Length: 462 bp

>NTDB_id=1106986 ACLKM4_RS12670 WP_286396274.1 2640665..2641126(-) (comE) [Acinetobacter indicus strain D1_NDM_tet(X3)]
ATGAATAAAATGAATAAATTTGCTGAACGAGGTTTTACTCTGATAGAACTTATGGTTGTGGTAGTAATTGTGGCAATTTT
TGCTGCAATTGCTATCCCAAGTTATCAGAATTATGTTCGGCGTGCAGAGGCATCCCAAGTGCAACAAGAAATTCAAAGAT
TAGCCAGTGAATTGTCTAAGTGGAAGTCTCGGAATTTCAGTTATCAAGGATTCAATTTAACGCCACAAACCATAAGAAAT
TACACTATTGAAATCAAAGATGGAACCGATACTGCTTTTAAACTAGATGATAAAGATGGTACTGAATACAAGGCTAATGG
CCAGCAATGGGTAATTAAAGCAATTCATGCAGATGGAACGAATGTCTCTTTCTTGCAAACCAGTACAGGATTAAAGTGTA
AAAATAAAGCAAAGGCAAATATTACCTATAGCAGCTGTGGTACAGGGGGTGAAGAATGGTGA

Domains

Predicted by InterproScan.

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (33-95)

• Prokaryotic N-terminal methylation motif (N_methyl) (9-31)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Acinetobacter baylyi ADP1	49.39	100	0.529
pilY2	Acinetobacter baumannii D1279779	43.373	100	0.471