Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ACLKM6_RS12470 Genome accession   NZ_CP183890
Coordinates   2589512..2589958 (-) Length   148 a.a.
NCBI ID   WP_104490277.1    Uniprot ID   -
Organism   Acinetobacter indicus strain D11_NDM_tet(X5)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2589966..2606033 2589512..2589958 flank 8


Gene organization within MGE regions


Location: 2589512..2606033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM6_RS12470 comE 2589512..2589958 (-) 447 WP_104490277.1 type IV pilin protein Machinery gene
  ACLKM6_RS12475 - 2589966..2593757 (-) 3792 WP_411686689.1 pilus assembly protein PilY -
  ACLKM6_RS12480 - 2593773..2594537 (-) 765 WP_171297593.1 pilus assembly PilX family protein -
  ACLKM6_RS12485 - 2594534..2595556 (-) 1023 WP_411686688.1 PilW family protein -
  ACLKM6_RS12490 pilV 2595557..2596063 (-) 507 WP_411686687.1 type IV pilus modification protein PilV -
  ACLKM6_RS12495 - 2596063..2596509 (-) 447 WP_411686686.1 GspH/FimT family pseudopilin -
  ACLKM6_RS12500 ispH 2596673..2597623 (-) 951 WP_045795274.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ACLKM6_RS12505 gmk 2597759..2598382 (+) 624 WP_411686685.1 guanylate kinase -
  ACLKM6_RS12510 rpoZ 2598459..2598740 (+) 282 WP_005013174.1 DNA-directed RNA polymerase subunit omega -
  ACLKM6_RS12515 - 2598963..2601062 (+) 2100 WP_168417090.1 RelA/SpoT family protein -
  ACLKM6_RS12520 - 2601134..2601517 (+) 384 WP_104513240.1 RidA family protein -
  ACLKM6_RS12525 - 2601672..2601866 (+) 195 WP_034597991.1 bacterioferritin-associated ferredoxin -
  ACLKM6_RS12530 bfr 2602093..2602557 (+) 465 WP_005180468.1 heteropolymeric bacterioferritin subunit Bfr -
  ACLKM6_RS12535 - 2602669..2604309 (-) 1641 WP_411686684.1 PglL family O-oligosaccharyltransferase -
  ACLKM6_RS12540 - 2604390..2606033 (-) 1644 WP_411686683.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16377.66 Da        Isoelectric Point: 9.9485

>NTDB_id=1106903 ACLKM6_RS12470 WP_104490277.1 2589512..2589958(-) (comE) [Acinetobacter indicus strain D11_NDM_tet(X5)]
MKKSKSSYAGFTLIELMVVIVIVAILATIAIPSYQEYVRRANASQAQDQLQQIAILLDKHKSRNFNYLDFVLASNLTTLP
QNAVGSAIKYNFTVNTGNGQSWVITAQSQDSRNFSFLMSSSGVRCKNKTWTNINASTVTCGTAGREEW

Nucleotide


Download         Length: 447 bp        

>NTDB_id=1106903 ACLKM6_RS12470 WP_104490277.1 2589512..2589958(-) (comE) [Acinetobacter indicus strain D11_NDM_tet(X5)]
ATGAAAAAGAGTAAATCATCTTATGCGGGCTTTACTTTAATAGAATTGATGGTTGTGATTGTGATTGTAGCTATTTTAGC
TACAATCGCGATCCCCTCGTATCAGGAATATGTCCGCCGTGCGAATGCAAGTCAAGCCCAAGATCAATTGCAGCAAATTG
CAATACTGCTTGATAAACATAAATCACGAAACTTTAATTATTTAGATTTTGTTTTGGCCTCGAATTTAACAACACTTCCT
CAAAATGCAGTTGGGAGCGCTATCAAATATAATTTTACAGTTAATACAGGTAATGGGCAGTCTTGGGTAATTACAGCACA
AAGTCAGGATAGTCGAAATTTTAGTTTTCTAATGTCGAGCTCGGGAGTACGCTGCAAGAATAAGACATGGACAAATATTA
ATGCCTCTACTGTGACATGTGGAACAGCGGGACGTGAAGAATGGTAA

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(32-112)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Acinetobacter baylyi ADP1

42.941

100

0.493
  pilY2 Acinetobacter baumannii D1279779

49.286

94.595

0.466

Multiple sequence alignment