Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACNSPI_RS08295 Genome accession   NZ_CP183317
Coordinates   1601887..1602573 (+) Length   228 a.a.
NCBI ID   WP_001792568.1    Uniprot ID   A0A133PXY3
Organism   Staphylococcus aureus strain USA300     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1596887..1607573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNSPI_RS08260 (ACNSPI_08260) yqeH 1597414..1598514 (+) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -
  ACNSPI_RS08265 (ACNSPI_08265) aroE 1598528..1599334 (+) 807 WP_000666761.1 shikimate dehydrogenase -
  ACNSPI_RS08270 (ACNSPI_08270) yhbY 1599338..1599628 (+) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  ACNSPI_RS08275 (ACNSPI_08275) nadD 1599631..1600200 (+) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  ACNSPI_RS08280 (ACNSPI_08280) yqeK 1600190..1600774 (+) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACNSPI_RS08285 (ACNSPI_08285) rsfS 1600775..1601128 (+) 354 WP_001088022.1 ribosome silencing factor -
  ACNSPI_RS08290 (ACNSPI_08290) - 1601131..1601847 (+) 717 WP_000084829.1 class I SAM-dependent DNA methyltransferase -
  ACNSPI_RS08295 (ACNSPI_08295) comEA 1601887..1602573 (+) 687 WP_001792568.1 ComEA family DNA-binding protein Machinery gene
  ACNSPI_RS08300 (ACNSPI_08300) - 1602665..1603126 (+) 462 WP_000439693.1 ComE operon protein 2 -
  ACNSPI_RS08305 (ACNSPI_08305) comEC 1603131..1605332 (+) 2202 WP_001548978.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACNSPI_RS08310 (ACNSPI_08310) holA 1605389..1606363 (+) 975 WP_001282562.1 DNA polymerase III subunit delta -
  ACNSPI_RS08315 (ACNSPI_08315) rpsT 1606408..1606659 (-) 252 WP_001274017.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=1105252 ACNSPI_RS08295 WP_001792568.1 1601887..1602573(+) (comEA) [Staphylococcus aureus strain USA300]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=1105252 ACNSPI_RS08295 WP_001792568.1 1601887..1602573(+) (comEA) [Staphylococcus aureus strain USA300]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133PXY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.238

99.561

0.978
  comEA Staphylococcus aureus N315

97.357

99.561

0.969

Multiple sequence alignment