Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilY1   Type   Machinery gene
Locus tag   ACAK32_RS17725 Genome accession   NZ_AP031580
Coordinates   3698489..3702343 (-) Length   1284 a.a.
NCBI ID   WP_000768970.1    Uniprot ID   A0A7H2A3V4
Organism   Acinetobacter baumannii strain JUNP419     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3693489..3707343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAK32_RS17690 (JUNP419_3552) - 3694249..3695190 (+) 942 WP_085916958.1 triacylglycerol lipase -
  ACAK32_RS17695 (JUNP419_3553) rplS 3695243..3695611 (-) 369 WP_000014562.1 50S ribosomal protein L19 -
  ACAK32_RS17700 (JUNP419_3554) trmD 3695820..3696560 (-) 741 WP_000464598.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ACAK32_RS17705 (JUNP419_3555) rimM 3696606..3697154 (-) 549 WP_000189236.1 ribosome maturation factor RimM -
  ACAK32_RS17710 (JUNP419_3556) rpsP 3697174..3697425 (-) 252 WP_000260334.1 30S ribosomal protein S16 -
  ACAK32_RS17715 (JUNP419_3557) pilE 3697572..3697997 (-) 426 WP_000788342.1 type IV pilin protein Machinery gene
  ACAK32_RS17720 (JUNP419_3558) pilY2 3697994..3698476 (-) 483 WP_001046420.1 type IV pilin protein Machinery gene
  ACAK32_RS17725 (JUNP419_3559) pilY1 3698489..3702343 (-) 3855 WP_000768970.1 PilC/PilY family type IV pilus protein Machinery gene
  ACAK32_RS17730 (JUNP419_3560) pilX 3702355..3703173 (-) 819 WP_000149374.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  ACAK32_RS17735 (JUNP419_3561) pilW 3703170..3704171 (-) 1002 WP_000079200.1 PilW family protein Machinery gene
  ACAK32_RS17740 (JUNP419_3562) pilV 3704172..3704732 (-) 561 WP_085916952.1 type IV pilus modification protein PilV Machinery gene
  ACAK32_RS17745 (JUNP419_3563) fimU 3704726..3705199 (-) 474 WP_001214061.1 Tfp pilus assembly protein FimT/FimU Machinery gene
  ACAK32_RS17750 (JUNP419_3564) ispH 3705369..3706319 (-) 951 WP_000407064.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ACAK32_RS17755 (JUNP419_3565) gmk 3706442..3707071 (+) 630 WP_000015937.1 guanylate kinase -

Sequence

Protein


Download         Length: 1284 a.a.        Molecular weight: 138259.74 Da        Isoelectric Point: 7.7712

>NTDB_id=110285 ACAK32_RS17725 WP_000768970.1 3698489..3702343(-) (pilY1) [Acinetobacter baumannii strain JUNP419]
MKLKLKNFKPNNLWYAVYSSSMIFTWLMTSSVVQASDLQIYASPTAGKKTIVMMLDTSGSMTNNSYGENRLAMLKNGMNA
FLASNNPVLNDTRVGLGNFSANGDSRSGQILVAAAPLGDASTLNTVGSQRYKLKQAVANLTAGGSTPSAHAYAEAAAYLM
GTTTYSETNYAIRKDSYIKRVRRSDNRTEYSYCTNYRDSQIDTANLWQPCRSNSYWSSWSTNNPGVDNATAYDTSSDWTY
YYTYYYTTFNYAVANADSGIPKSKSNDTASNPNIVVDRNATNSNAVYQSPLPAVANRQSCDGQGIYFLSDGEPNNTTNTR
SASVMSTALGSTFGADFNCSGGLSNTTADSGWACMGEFAKRLFDKTKNPAGVSIQTAFVGFGSDFSSLSSSDVKNACRLS
SRTQSDRKGDDACSPNQSTNAVAAPGYGNGGFFPTQSSQGVTDSVIAFINNLDKVPLEPLTTGAISVPYDALNPKNLQEY
GYLRAFEPNPANTYLTWRGNLKKYHVVLSGANAGTFEANTGGLVYNATGAFRTGTKDYWNSSTYNDGGKVFFGGSYAKVP
LPIAGQPETRDEEGNITKYYYAVQTKIRNLFTDVSAVASDGSLTKISTSGTNLLKIPAAPPEGTNPFDTVANTASYVLGK
FDPSTGQNILKAFPISLKLKILNYLGYSTDINATTLPSSLVTSNEPYLSMGGSIHSLPVQLTYNGTLDDNGNLTSAREQS
ILYGTMEGGLHIVDASSGIEQMVFVPADILNDSVASKALVVGQSDASAPAHGMDGAWVSDPAYNITTVGSGSSAVSKVTA
KQMNIYGGMRMGGSSYYGLDVLSPTSPKLLFRIGADQTDYSRMGQSWSKPVLANIRYNGSIRRVLIVGGGYDQCYEKPNI
TLTDACFTNGKAKGNAVYIIDAKTGQRLWWTSDTGSNTDNANMKHSIVSRISTLDRDADGLVDHLYFGDLGGQIFRVDLN
NNQTKTNSTYSSFGVRVVRLANLATNDSTYDGTNDYTGGNAPRFYEPPTVTIHDYGIHTFITLGIASGDRSTPLDVYPLT
GREGMTPASALSGRPVNNVYGIIDRDFVKKNLMSLTDNQLETKDITRTGLRKNPQILRTGETRVAQIFFPTTGVGKGGWY
RSLSSTSDGTEKANNSFRIKGGLKAFEEPMAITGNLIIPVYDPQGTGIVAADPCLPRVVGETDRQTYCLPFGACLNPDGT
INSNKENPSGFQTKTGSNCPAGVSECNTNVIGAGIRNITFVPKRDEPPVTNSCGKLQLSGNENGTGEWQCTSHLLPVRWY
ERYR

Nucleotide


Download         Length: 3855 bp        

>NTDB_id=110285 ACAK32_RS17725 WP_000768970.1 3698489..3702343(-) (pilY1) [Acinetobacter baumannii strain JUNP419]
ATGAAACTAAAATTGAAGAATTTTAAGCCAAATAATTTGTGGTATGCCGTCTATTCAAGCTCAATGATATTCACATGGCT
AATGACAAGCTCTGTGGTGCAAGCAAGTGATTTACAAATTTATGCCTCACCTACAGCAGGTAAAAAAACAATTGTGATGA
TGTTGGATACATCAGGAAGTATGACCAACAATAGTTATGGTGAAAACCGCTTAGCCATGCTTAAAAATGGTATGAATGCT
TTTTTGGCTAGCAATAACCCTGTCTTAAATGATACGCGAGTAGGTTTAGGAAATTTCTCGGCAAATGGTGATAGCCGAAG
TGGACAAATTTTAGTGGCTGCTGCTCCTTTAGGTGATGCAAGTACTTTAAATACGGTAGGCTCACAACGTTATAAATTAA
AACAAGCTGTTGCTAATTTAACTGCGGGAGGCTCAACACCTTCAGCCCACGCTTATGCTGAAGCAGCTGCTTACTTAATG
GGGACAACCACATATTCAGAGACGAATTATGCTATTCGTAAAGATAGTTATATCAAACGTGTAAGAAGATCTGATAATAG
AACTGAATATTCATATTGTACTAATTACCGTGATTCGCAAATTGATACAGCCAACCTATGGCAACCTTGTCGTTCAAATA
GTTATTGGAGTAGCTGGTCAACAAATAATCCAGGTGTGGATAATGCTACTGCTTATGATACTTCATCTGACTGGACTTAT
TACTATACTTATTACTACACCACTTTTAATTATGCTGTAGCAAATGCAGATAGCGGTATTCCAAAATCTAAATCAAACGA
TACAGCTAGCAATCCGAATATTGTTGTAGACCGAAATGCTACTAACTCCAATGCAGTGTATCAGTCACCTTTACCTGCAG
TAGCGAATCGCCAGAGTTGTGATGGGCAAGGTATTTACTTCTTATCGGATGGCGAACCGAACAATACAACAAATACACGT
TCGGCAAGTGTCATGTCGACTGCTTTAGGTAGTACTTTTGGAGCTGATTTTAATTGTTCTGGAGGTTTATCTAACACTAC
GGCAGACTCTGGTTGGGCTTGTATGGGGGAGTTTGCAAAAAGATTATTTGATAAAACAAAGAACCCTGCCGGAGTTTCTA
TTCAAACAGCATTTGTCGGTTTTGGTAGTGACTTTTCTAGTTTGAGTTCTTCTGATGTAAAAAATGCCTGCCGTTTAAGT
TCGAGAACCCAGTCTGACCGAAAAGGAGATGATGCGTGTTCACCGAATCAATCTACAAATGCGGTAGCTGCACCAGGTTA
TGGGAATGGTGGTTTTTTCCCTACTCAGAGTTCACAAGGCGTAACAGACAGCGTTATCGCATTTATTAATAATTTAGATA
AAGTTCCTTTAGAACCCTTAACCACAGGTGCTATCTCAGTTCCATATGATGCATTGAATCCCAAAAATTTACAGGAATAT
GGTTATTTACGAGCTTTTGAGCCAAATCCAGCTAACACGTACTTAACATGGCGTGGAAATTTAAAGAAATATCATGTTGT
TTTATCTGGAGCGAATGCAGGTACTTTTGAAGCTAATACTGGTGGGTTGGTTTACAACGCAACTGGGGCATTTAGAACGG
GTACAAAAGATTATTGGAATAGCTCTACTTATAATGATGGAGGTAAAGTCTTTTTTGGTGGTTCATATGCAAAAGTTCCT
TTACCCATTGCTGGACAACCTGAAACACGTGATGAAGAAGGAAATATCACAAAATATTATTACGCAGTACAAACTAAAAT
CCGCAACTTATTTACTGATGTTTCCGCCGTCGCATCAGATGGTAGCTTAACGAAAATTTCAACTTCTGGAACTAATTTGC
TAAAAATTCCAGCTGCTCCACCAGAAGGAACTAATCCTTTTGATACGGTGGCTAATACAGCAAGTTATGTTTTAGGTAAA
TTTGATCCATCAACTGGACAAAATATTTTAAAAGCTTTTCCTATAAGCTTGAAATTAAAAATATTAAATTATTTAGGTTA
TTCAACAGATATTAATGCAACAACTCTGCCTTCATCTTTGGTTACATCGAATGAACCTTATTTATCGATGGGGGGAAGTA
TTCACTCTTTACCGGTTCAACTAACCTACAATGGAACACTAGATGATAATGGGAATTTAACATCTGCCCGAGAACAATCC
ATCCTCTATGGAACTATGGAAGGCGGATTACATATTGTGGATGCTTCATCTGGTATTGAGCAAATGGTCTTTGTTCCTGC
AGATATTTTAAATGATTCAGTTGCTTCCAAAGCTTTAGTTGTTGGTCAAAGTGATGCTTCAGCTCCCGCTCATGGTATGG
ACGGAGCTTGGGTATCTGATCCAGCCTATAATATTACTACAGTGGGAAGTGGTAGCTCGGCAGTATCAAAAGTAACTGCA
AAGCAAATGAATATTTATGGCGGCATGCGTATGGGAGGAAGTAGCTACTACGGACTAGATGTATTAAGCCCTACTTCACC
GAAACTGCTTTTTAGAATAGGGGCAGACCAGACTGACTATAGCCGTATGGGTCAAAGCTGGTCTAAACCCGTACTCGCGA
ACATCCGTTATAACGGTTCTATTAGACGCGTCCTGATTGTTGGCGGTGGTTATGATCAGTGTTATGAAAAACCAAATATT
ACGTTGACTGACGCTTGCTTTACCAATGGAAAAGCAAAAGGAAATGCTGTCTATATTATTGACGCAAAAACTGGTCAGCG
TTTGTGGTGGACAAGTGATACAGGTTCTAATACTGATAACGCCAATATGAAGCATAGTATTGTTAGCCGTATTAGTACTT
TAGACCGTGATGCTGATGGCTTAGTCGACCATCTATATTTTGGAGATTTAGGCGGACAAATTTTTCGCGTGGACCTTAAT
AATAATCAGACAAAAACCAATTCGACCTATAGCAGTTTTGGTGTCAGAGTTGTGCGTTTAGCGAATTTAGCAACAAATGA
TTCAACTTATGATGGCACAAATGATTATACAGGTGGGAATGCTCCTCGTTTTTATGAGCCTCCAACAGTAACGATCCACG
ATTATGGAATTCACACTTTTATTACATTAGGAATTGCATCAGGAGACCGTAGTACACCTTTAGATGTTTACCCACTCACA
GGTCGTGAAGGTATGACGCCTGCAAGTGCATTAAGTGGACGTCCTGTAAATAATGTATATGGAATTATTGATAGAGACTT
TGTTAAAAAGAACTTAATGTCTTTAACTGATAATCAGCTTGAAACAAAAGACATTACACGGACAGGCTTAAGAAAAAATC
CACAAATTCTAAGAACAGGTGAAACAAGAGTAGCACAAATTTTCTTCCCCACTACAGGAGTAGGTAAAGGTGGTTGGTAT
CGCTCGCTTTCTAGTACGAGTGATGGTACAGAAAAAGCTAATAATAGTTTTCGTATTAAAGGAGGACTCAAAGCTTTTGA
AGAACCAATGGCAATTACGGGTAATTTAATTATTCCAGTTTATGACCCTCAAGGAACGGGAATTGTTGCGGCAGATCCTT
GTCTACCTCGTGTTGTGGGAGAAACAGACCGACAAACTTATTGTTTACCATTTGGTGCTTGTCTTAATCCGGATGGAACC
ATTAATTCAAATAAAGAAAACCCGAGCGGGTTTCAAACAAAAACTGGTAGTAACTGTCCAGCTGGAGTCTCTGAATGTAA
TACAAATGTCATTGGTGCAGGTATTCGTAATATCACATTTGTTCCAAAAAGAGATGAACCTCCTGTGACTAATAGTTGTG
GGAAATTACAGCTGTCTGGAAACGAGAACGGAACAGGTGAGTGGCAATGTACAAGTCATTTACTTCCTGTGCGCTGGTAT
GAGCGCTATCGCTAG

Domains

Predicted by InterproScan.

Neisseria PilC beta-propeller domain (T4P_PilY1)

(715-964)

von Willebrand factor type A domain (VWA_2)

(51-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilY1 Acinetobacter baumannii D1279779

88.527

100

0.889

Multiple sequence alignment