Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ACNFKJ_RS00555 Genome accession   NZ_CP182514
Coordinates   120792..121289 (-) Length   165 a.a.
NCBI ID   WP_001794328.1    Uniprot ID   -
Organism   Staphylococcus aureus strain subspecie aureus F-182     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 115792..126289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNFKJ_RS00530 gcvPB 115954..117426 (-) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  ACNFKJ_RS00535 gcvPA 117419..118765 (-) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ACNFKJ_RS00540 gcvT 118785..119876 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  ACNFKJ_RS00545 - 120035..120559 (-) 525 WP_001015125.1 shikimate kinase -
  ACNFKJ_RS00550 - 120549..120695 (-) 147 WP_001789879.1 hypothetical protein -
  ACNFKJ_RS00555 comGF 120792..121289 (-) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACNFKJ_RS00560 comGE 121207..121506 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACNFKJ_RS00565 comGD 121493..121939 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACNFKJ_RS00570 comGC 121917..122228 (-) 312 WP_061843224.1 competence type IV pilus major pilin ComGC Machinery gene
  ACNFKJ_RS00575 comGB 122242..123312 (-) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACNFKJ_RS00580 comGA 123284..124258 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACNFKJ_RS00585 - 124310..124933 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ACNFKJ_RS00590 - 124930..125259 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACNFKJ_RS00595 - 125259..126245 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19197.95 Da        Isoelectric Point: 10.2192

>NTDB_id=1101779 ACNFKJ_RS00555 WP_001794328.1 120792..121289(-) (comGF) [Staphylococcus aureus strain subspecie aureus F-182]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1101779 ACNFKJ_RS00555 WP_001794328.1 120792..121289(-) (comGF) [Staphylococcus aureus strain subspecie aureus F-182]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

97.576

100

0.976

Multiple sequence alignment