Detailed information NTG1099248

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACM599_RS07535
• Genome accession: NZ_CP181152
• Coordinates: 1590499..1590996 (-)
• Length: 165 a.a.
• NCBI ID: WP_001794055.1
• Uniprot ID: A0A7U7EV69
• Organism: Staphylococcus aureus strain AG21-0597
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1585499..1595996

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACM599_RS07510 (ACM599_07510)	gcvPB	1585661..1587133 (-)	1473	WP_000202178.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
ACM599_RS07515 (ACM599_07515)	gcvPA	1587126..1588472 (-)	1347	WP_000019697.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACM599_RS07520 (ACM599_07520)	gcvT	1588492..1589583 (-)	1092	WP_000093347.1	glycine cleavage system aminomethyltransferase GcvT	-
ACM599_RS07525 (ACM599_07525)	-	1589742..1590266 (-)	525	WP_001015125.1	shikimate kinase	-
ACM599_RS07530 (ACM599_07530)	-	1590256..1590402 (-)	147	WP_001789879.1	hypothetical protein	-
ACM599_RS07535 (ACM599_07535)	comGF	1590499..1590996 (-)	498	WP_001794055.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACM599_RS07540 (ACM599_07540)	comGE	1590914..1591213 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACM599_RS07545 (ACM599_07545)	comGD	1591200..1591646 (-)	447	WP_001791207.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACM599_RS07550 (ACM599_07550)	comGC	1591624..1591935 (-)	312	WP_000472257.1	competence type IV pilus major pilin ComGC	Machinery gene
ACM599_RS07555 (ACM599_07555)	comGB	1591949..1593019 (-)	1071	WP_000776412.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACM599_RS07560 (ACM599_07560)	comGA	1592991..1593965 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACM599_RS07565 (ACM599_07565)	-	1594017..1594640 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACM599_RS07570 (ACM599_07570)	-	1594637..1594966 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACM599_RS07575 (ACM599_07575)	-	1594966..1595952 (-)	987	WP_000161314.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19224.98 Da
• Isoelectric Point: 10.2192

>NTDB_id=1099248 ACM599_RS07535 WP_001794055.1 1590499..1590996(-) (comGF) [Staphylococcus aureus strain AG21-0597]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLNKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1099248 ACM599_RS07535 WP_001794055.1 1590499..1590996(-) (comGF) [Staphylococcus aureus strain AG21-0597]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAACAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A7U7EV69

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97