Detailed information NTG1098174

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACM6AU_RS07280
• Genome accession: NZ_CP181097
• Coordinates: 1529886..1530383 (-)
• Length: 165 a.a.
• NCBI ID: WP_001796472.1
• Uniprot ID: A0A5F0HJ55
• Organism: Staphylococcus aureus strain AG21-0627
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1524886..1535383

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACM6AU_RS07255 (ACM6AU_07255)	gcvPB	1525048..1526520 (-)	1473	WP_000202176.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
ACM6AU_RS07260 (ACM6AU_07260)	gcvPA	1526513..1527859 (-)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACM6AU_RS07265 (ACM6AU_07265)	gcvT	1527879..1528970 (-)	1092	WP_000093344.1	glycine cleavage system aminomethyltransferase GcvT	-
ACM6AU_RS07270 (ACM6AU_07270)	-	1529129..1529653 (-)	525	WP_001015120.1	shikimate kinase	-
ACM6AU_RS07275 (ACM6AU_07275)	-	1529643..1529789 (-)	147	WP_001789879.1	hypothetical protein	-
ACM6AU_RS07280 (ACM6AU_07280)	comGF	1529886..1530383 (-)	498	WP_001796472.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACM6AU_RS07285 (ACM6AU_07285)	comGE	1530301..1530600 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACM6AU_RS07290 (ACM6AU_07290)	comGD	1530587..1531033 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACM6AU_RS07295 (ACM6AU_07295)	comGC	1531011..1531322 (-)	312	WP_000472255.1	competence type IV pilus major pilin ComGC	Machinery gene
ACM6AU_RS07300 (ACM6AU_07300)	comGB	1531336..1532406 (-)	1071	WP_000776409.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACM6AU_RS07305 (ACM6AU_07305)	comGA	1532378..1533352 (-)	975	WP_000697223.1	competence type IV pilus ATPase ComGA	Machinery gene
ACM6AU_RS07310 (ACM6AU_07310)	-	1533404..1534027 (-)	624	WP_001223009.1	MBL fold metallo-hydrolase	-
ACM6AU_RS07315 (ACM6AU_07315)	-	1534024..1534353 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACM6AU_RS07320 (ACM6AU_07320)	-	1534353..1535339 (-)	987	WP_000161307.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19169.89 Da
• Isoelectric Point: 10.1358

>NTDB_id=1098174 ACM6AU_RS07280 WP_001796472.1 1529886..1530383(-) (comGF) [Staphylococcus aureus strain AG21-0627]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1098174 ACM6AU_RS07280 WP_001796472.1 1529886..1530383(-) (comGF) [Staphylococcus aureus strain AG21-0627]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (68-155)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

3D structure

• AlphaFold DB: A0A5F0HJ55

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	95.758	100	0.958