Detailed information NTG1096952

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACM2W2_RS22320
• Genome accession: NZ_CP180596
• Coordinates: 4778276..4779148 (+)
• Length: 290 a.a.
• NCBI ID: WP_043085203.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 60
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4773276..4784148

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACM2W2_RS22300	-	4773819..4774376 (-)	558	WP_224754157.1	hypothetical protein	-
ACM2W2_RS22305	-	4774581..4775120 (-)	540	WP_033938465.1	pilin	-
ACM2W2_RS22310	pilB	4775349..4777052 (+)	1704	WP_003116249.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACM2W2_RS22315	pilC	4777056..4778273 (+)	1218	WP_003116250.1	type II secretion system F family protein	Machinery gene
ACM2W2_RS22320	pilD	4778276..4779148 (+)	873	WP_043085203.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
ACM2W2_RS22325	coaE	4779145..4779756 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
ACM2W2_RS22330	yacG	4779753..4779953 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
ACM2W2_RS22335	-	4779990..4780199 (-)	210	WP_003094660.1	hypothetical protein	-
ACM2W2_RS22340	-	4780305..4780994 (-)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
ACM2W2_RS22345	-	4780991..4781461 (-)	471	WP_003094664.1	hypothetical protein	-
ACM2W2_RS22350	-	4781458..4781883 (-)	426	WP_021264128.1	GNAT family N-acetyltransferase	-
ACM2W2_RS22355	-	4782016..4782645 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
ACM2W2_RS22360	-	4782642..4783091 (+)	450	WP_021264127.1	MOSC domain-containing protein	-
ACM2W2_RS22365	-	4783117..4783287 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31855.84 Da
• Isoelectric Point: 7.0816

>NTDB_id=1096952 ACM2W2_RS22320 WP_043085203.1 4778276..4779148(+) (pilD) [Pseudomonas aeruginosa strain 60]
MELLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPSSACPHCGHEIR
PWENIPLVSYLALGGKCSSCKATIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=1096952 ACM2W2_RS22320 WP_043085203.1 4778276..4779148(+) (pilD) [Pseudomonas aeruginosa strain 60]
ATGGAACTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCAGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAGTTCGGCGTGCCCGCACTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCACCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.363	99.655	0.552
pilD	Vibrio campbellii strain DS40M4	53.497	98.621	0.528
pilD	Acinetobacter nosocomialis M2	50.352	97.931	0.493
pilD	Acinetobacter baumannii D1279779	50.352	97.931	0.493
pilD	Neisseria gonorrhoeae MS11	49.281	95.862	0.472