Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACLCDX_RS11605 Genome accession   NZ_CP180263
Coordinates   2289103..2290173 (-) Length   356 a.a.
NCBI ID   WP_025092972.1    Uniprot ID   A0A5C0WKU5
Organism   Bacillus safensis strain BS22LVI     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2284103..2295173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLCDX_RS11560 (ACLCDX_11560) sipW 2284192..2284764 (-) 573 WP_024422971.1 signal peptidase I SipW -
  ACLCDX_RS11565 (ACLCDX_11565) tapA 2284817..2285344 (-) 528 WP_098677430.1 amyloid fiber anchoring/assembly protein TapA -
  ACLCDX_RS11570 (ACLCDX_11570) - 2285644..2285922 (+) 279 WP_171464609.1 DUF3889 domain-containing protein -
  ACLCDX_RS11575 (ACLCDX_11575) - 2285956..2286150 (-) 195 WP_056767455.1 YqzE family protein -
  ACLCDX_RS11580 (ACLCDX_11580) - 2286217..2286600 (-) 384 WP_197172668.1 competence type IV pilus minor pilin ComGG -
  ACLCDX_RS11585 (ACLCDX_11585) comGF 2286597..2287349 (-) 753 WP_415315994.1 competence type IV pilus minor pilin ComGF -
  ACLCDX_RS11590 (ACLCDX_11590) comGD 2287333..2287779 (-) 447 WP_197172667.1 competence type IV pilus minor pilin ComGD -
  ACLCDX_RS11595 (ACLCDX_11595) comGC 2287772..2288065 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  ACLCDX_RS11600 (ACLCDX_11600) comGB 2288082..2289122 (-) 1041 WP_197172666.1 competence type IV pilus assembly protein ComGB -
  ACLCDX_RS11605 (ACLCDX_11605) comGA 2289103..2290173 (-) 1071 WP_025092972.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLCDX_RS11610 (ACLCDX_11610) - 2290351..2291226 (-) 876 WP_024422979.1 STAS domain-containing protein -
  ACLCDX_RS11620 (ACLCDX_11620) - 2291450..2291830 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACLCDX_RS11625 (ACLCDX_11625) - 2292043..2292285 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  ACLCDX_RS11630 (ACLCDX_11630) - 2292333..2292971 (-) 639 WP_024422981.1 MBL fold metallo-hydrolase -
  ACLCDX_RS11635 (ACLCDX_11635) - 2293129..2293302 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  ACLCDX_RS11640 (ACLCDX_11640) - 2293327..2293641 (-) 315 WP_024422982.1 MTH1187 family thiamine-binding protein -
  ACLCDX_RS11645 (ACLCDX_11645) - 2293718..2294971 (-) 1254 WP_415315995.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40412.86 Da        Isoelectric Point: 9.5360

>NTDB_id=1094977 ACLCDX_RS11605 WP_025092972.1 2289103..2290173(-) (comGA) [Bacillus safensis strain BS22LVI]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1094977 ACLCDX_RS11605 WP_025092972.1 2289103..2290173(-) (comGA) [Bacillus safensis strain BS22LVI]
TTGTATGGGATTGAATATTTAAGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAACCATTGATAAAAAAAGCGGAG
AGCGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATAGGGGAAAAAAGGAGGCCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGTCAAGTATTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACCAATTCGGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTCGAAACAAGAAATGAAGAAGTATTACAGGTTCA
AGTAAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATATTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCCCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTTGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGTCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WKU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643

Multiple sequence alignment