Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   ACMUEV_RS02540 Genome accession   NZ_CP180258
Coordinates   547764..550403 (+) Length   879 a.a.
NCBI ID   WP_017290866.1    Uniprot ID   -
Organism   Leptolyngbya sp. FACHB-1624     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 542764..555403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMUEV_RS02520 (ACMUEV_02520) hmpF 543314..545095 (-) 1782 WP_190648442.1 pilus motility taxis protein HmpF -
  ACMUEV_RS02525 (ACMUEV_02525) - 545240..546472 (+) 1233 WP_190648439.1 response regulator -
  ACMUEV_RS02530 (ACMUEV_02530) pilH 546601..546966 (+) 366 WP_017290869.1 response regulator transcription factor Machinery gene
  ACMUEV_RS02535 (ACMUEV_02535) - 546978..547508 (+) 531 WP_017290868.1 chemotaxis protein CheW -
  ACMUEV_RS02540 (ACMUEV_02540) pilJ 547764..550403 (+) 2640 WP_017290866.1 methyl-accepting chemotaxis protein Machinery gene

Sequence

Protein


Download         Length: 879 a.a.        Molecular weight: 94289.08 Da        Isoelectric Point: 3.9936

>NTDB_id=1094876 ACMUEV_RS02540 WP_017290866.1 547764..550403(+) (pilJ) [Leptolyngbya sp. FACHB-1624]
MARGNEFAQEYQQAERAYMQGKYEEAAGIVDRLVDNYPQDPSARLLRGHIYCYGLQKYDVAQEQYQSVLDLTSDPEFVDY
ANSGLAYAGQFSDSSADSFADTSLETDYSGFDESAYLSQESANSLDDFAFDDDFKATTEESSSFGNPFGAQHNGDGSKVG
ASIGLDDPFAVSASSDAPFSMQDESVVSQDFSNALDDFDQVFASDDEPSHGSMSTVSTDPGMSQGDMAVPISRVTSRDTS
EETLFMAAPGFYDEQAAAAFEMPTEQNKFAPIYDEPQNGSRNHPVSTPGNQSNVDFLDDFEDFDDLGNLSDFDLSEDSAG
FTSPSIGSAFGFNSNMSAASDSAEVDFGQGSDFAPSNRDGSAFQDDELFSMSSTTEQVPSFTTQLDVPSSEPTPTVEQGW
LAPLENASFATKQLIAAGAAGIVSAVAVAVVSFVASNQAAQNNNGAVVSQLRTTGIVMALASGVCGGGAAFAVGQMTARH
MKRSTDDLKAQFDSVCQGNLQARATVFAEDELGQLSTNFNQMTRVILTTTSEAQRKAEEQEQAKEDLQRQVIRLLDDVEG
AARGDLTVQAEVTADVLGAVADSFNLTIQNLREIVQQVKQAARQVSKGSTENEMFARSLSSDALRQAEELAVTLNSVQVM
TNSIQRVAESAREAEEVARTASSTALKGGEAVERTVAGILEIRETVAETTRKVKRLAESSQEISKIVALISQIASRTNLL
ALNASIEAARAGEAGRGFAIVADEVRQLADRAAKASKEIEQIVLQIQGETGSVMTAMEEGTQQVIEGTRLAEQAKRSLED
IIQVSSVIDTLVRSITADTVEQTETSRNVAQVMQSVELTAQETSQEAQRVSGSLQNLVGVARDLLTSVERFRVETVDRK

Nucleotide


Download         Length: 2640 bp        

>NTDB_id=1094876 ACMUEV_RS02540 WP_017290866.1 547764..550403(+) (pilJ) [Leptolyngbya sp. FACHB-1624]
ATGGCACGAGGAAATGAATTCGCTCAAGAATATCAGCAGGCAGAGCGCGCCTATATGCAGGGCAAGTATGAAGAAGCGGC
TGGCATCGTCGATCGCTTAGTTGATAATTATCCTCAAGATCCTAGTGCTCGACTCTTACGGGGTCACATCTACTGCTATG
GCTTACAAAAGTATGATGTGGCGCAAGAGCAATACCAGTCTGTTCTCGATCTCACCTCTGATCCAGAATTTGTTGATTAC
GCGAATAGTGGTCTGGCTTATGCGGGTCAATTTTCAGATAGCTCTGCCGATAGCTTTGCAGATACCAGTCTAGAAACAGA
TTATTCAGGATTTGACGAATCTGCTTATCTATCACAAGAATCTGCAAATTCACTGGATGACTTCGCATTCGATGACGATT
TCAAAGCAACTACAGAAGAATCGTCGTCTTTTGGAAATCCCTTTGGCGCACAGCACAACGGTGATGGTTCAAAAGTTGGA
GCTTCGATCGGGTTAGATGATCCGTTTGCAGTTTCAGCTTCATCGGATGCGCCGTTCTCAATGCAGGACGAATCCGTAGT
GAGCCAAGACTTCTCGAATGCACTGGATGATTTTGATCAAGTCTTTGCCTCAGATGATGAGCCTTCGCACGGCAGCATGT
CTACAGTTTCAACTGATCCAGGCATGTCCCAGGGAGACATGGCTGTTCCAATCAGTCGCGTTACAAGTCGGGATACCAGT
GAAGAAACCTTGTTTATGGCGGCTCCGGGCTTCTATGATGAGCAAGCTGCAGCTGCATTCGAGATGCCAACTGAGCAGAA
CAAATTTGCGCCGATCTACGATGAGCCGCAAAACGGCAGTCGAAATCATCCGGTCTCAACGCCAGGCAATCAAAGCAATG
TTGATTTCCTAGACGATTTTGAAGACTTTGATGATCTGGGAAATCTTTCAGATTTTGATCTATCTGAGGATTCAGCCGGA
TTTACCAGTCCTTCGATTGGAAGTGCGTTTGGATTCAATTCAAATATGTCCGCTGCTTCGGACAGCGCTGAAGTTGATTT
TGGGCAAGGTTCGGATTTTGCACCTTCTAATAGAGATGGTTCAGCCTTCCAGGATGATGAACTTTTCAGCATGTCCAGCA
CAACTGAACAGGTCCCAAGTTTTACGACTCAATTAGATGTTCCAAGTTCAGAACCGACTCCGACTGTTGAACAAGGTTGG
TTAGCTCCACTTGAGAATGCTTCATTCGCAACTAAGCAGTTGATTGCTGCTGGAGCTGCTGGGATTGTCTCAGCAGTAGC
GGTTGCAGTGGTTAGCTTTGTCGCTTCCAATCAGGCGGCTCAAAACAATAATGGAGCTGTTGTTTCTCAGCTGCGCACAA
CTGGGATTGTGATGGCTCTGGCGAGTGGTGTTTGCGGTGGTGGTGCAGCATTTGCGGTCGGTCAAATGACTGCTCGGCAT
ATGAAGCGATCGACCGATGACCTCAAAGCCCAATTTGATTCGGTCTGTCAGGGCAATCTTCAAGCACGGGCTACTGTTTT
TGCAGAAGATGAGCTTGGTCAACTCTCGACCAACTTTAACCAAATGACTCGTGTGATTCTGACTACGACCAGCGAGGCGC
AACGTAAGGCAGAAGAACAAGAGCAAGCGAAAGAAGATTTACAGCGCCAGGTGATTCGCTTACTCGATGATGTAGAAGGT
GCGGCTCGCGGTGATTTGACGGTACAAGCAGAGGTTACTGCTGACGTCCTTGGAGCGGTTGCTGACTCATTCAACTTAAC
CATTCAGAACCTGCGTGAAATCGTTCAACAGGTAAAACAAGCTGCACGTCAGGTCAGTAAGGGTTCCACGGAAAATGAGA
TGTTCGCTCGCAGTTTGTCTTCTGACGCATTGCGTCAAGCTGAAGAACTCGCGGTAACCCTGAACTCTGTACAGGTGATG
ACCAACTCAATTCAGCGTGTAGCAGAGAGCGCTCGTGAAGCCGAAGAAGTCGCAAGAACTGCGTCTTCGACTGCCTTGAA
GGGCGGTGAAGCGGTAGAACGTACTGTGGCTGGTATTCTCGAAATTCGAGAAACAGTGGCAGAAACGACGCGTAAAGTTA
AACGACTCGCGGAATCTTCGCAAGAGATTTCTAAGATTGTTGCCTTAATTTCGCAGATTGCGTCTCGAACCAATCTTCTT
GCATTGAACGCCAGTATTGAGGCAGCAAGAGCAGGAGAAGCAGGACGAGGATTTGCAATCGTTGCAGATGAAGTTCGACA
GTTAGCAGACCGAGCCGCAAAAGCGTCGAAAGAGATTGAACAAATCGTATTACAGATTCAAGGTGAAACTGGATCAGTAA
TGACCGCGATGGAAGAAGGAACTCAACAAGTTATCGAGGGAACCCGGCTCGCTGAACAAGCGAAGCGCTCACTCGAAGAC
ATTATTCAAGTATCGAGCGTCATTGACACACTTGTGAGATCAATTACCGCCGATACGGTTGAGCAGACCGAAACTTCACG
GAATGTCGCTCAAGTGATGCAATCCGTGGAATTGACTGCACAAGAGACTTCGCAAGAAGCTCAACGCGTGTCAGGATCAC
TGCAAAACCTAGTAGGGGTTGCGCGTGACTTGCTAACGTCGGTAGAACGATTCCGGGTAGAAACTGTTGATCGGAAGTAA

Domains

Predicted by InterproScan.

Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal)

(656-836)

HAMP domain (HAMP)

(477-524)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Synechocystis sp. PCC 6803

46.302

100

0.491

Multiple sequence alignment