Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ACLBK2_RS05905 Genome accession   NZ_CP180236
Coordinates   1134536..1138261 (+) Length   1241 a.a.
NCBI ID   WP_247998845.1    Uniprot ID   -
Organism   Bacillus sp. Ba 3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1129536..1143261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLBK2_RS05890 (ACLBK2_05890) - 1129694..1130287 (+) 594 WP_048526648.1 TVP38/TMEM64 family protein -
  ACLBK2_RS05895 (ACLBK2_05895) lepB 1130344..1130907 (+) 564 WP_048526650.1 signal peptidase I -
  ACLBK2_RS05900 (ACLBK2_05900) addB 1131024..1134539 (+) 3516 WP_247998844.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ACLBK2_RS05905 (ACLBK2_05905) addA 1134536..1138261 (+) 3726 WP_247998845.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACLBK2_RS05910 (ACLBK2_05910) - 1138274..1138561 (+) 288 WP_000255723.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  ACLBK2_RS05915 (ACLBK2_05915) - 1138655..1139032 (-) 378 WP_247998846.1 DUF1433 domain-containing protein -
  ACLBK2_RS05920 (ACLBK2_05920) - 1139091..1140260 (+) 1170 WP_098344764.1 hypothetical protein -
  ACLBK2_RS05925 (ACLBK2_05925) - 1140271..1140651 (+) 381 WP_098344763.1 hypothetical protein -
  ACLBK2_RS05930 (ACLBK2_05930) gerPF 1140930..1141145 (-) 216 WP_001141566.1 spore germination protein GerPF -
  ACLBK2_RS05935 (ACLBK2_05935) - 1141188..1141574 (-) 387 WP_048526663.1 spore germination protein GerPE -
  ACLBK2_RS05940 (ACLBK2_05940) gerPD 1141590..1141784 (-) 195 WP_001102342.1 spore germination protein GerPD -
  ACLBK2_RS05945 (ACLBK2_05945) gerPC 1141791..1142405 (-) 615 WP_247998847.1 spore germination protein GerPC -
  ACLBK2_RS05950 (ACLBK2_05950) gerPB 1142474..1142680 (-) 207 WP_001012508.1 spore germination protein GerPB -
  ACLBK2_RS05955 (ACLBK2_05955) gerPA 1142695..1142916 (-) 222 WP_001111188.1 spore germination protein GerPA -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142650.76 Da        Isoelectric Point: 4.8816

>NTDB_id=1094834 ACLBK2_RS05905 WP_247998845.1 1134536..1138261(+) (addA) [Bacillus sp. Ba 3]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVRFQLETAEQHI
RKATELAMLTDGPAPRVETLQADLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEHSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVPEGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLHDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPAEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKDEVYDRLMWKYGYGEAASHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIQTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQINGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=1094834 ACLBK2_RS05905 WP_247998845.1 1134536..1138261(+) (addA) [Bacillus sp. Ba 3]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCTGGTTCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAACCAGGCTCTCAGCACATTAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCTATTTCGACGATCCATTCCTTTTGTTTACAAGTAATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACGGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAGCTCGTTGATCGTTATACGAGTGATCGTAGTGACGATGATTTACAGCGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCACATCCAAATCCGGAAAAGTGGCTCGATAAATTAGTAGAAGCATATGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTATTTATTAGAAGATGTGAGATTCCAGCTTGAAACAGCGGAACAGCATATT
CGGAAAGCGACGGAACTCGCAATGCTTACTGACGGTCCAGCGCCTCGCGTTGAAACACTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCATCAGCCGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATATTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACATAGTGAAAACGGTGAAATGAATCCGTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCTATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTAGGTGACGTAAAACAGTCAATTTATCGTTTCCGACTAGCAG
AACCAGGTTTATTCCTAGGAAAGTATAAACGCTTTACACAAGAAGGATCGGGCGGCGGAATGAAGATAGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGCTAGCAGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGACTACGATGCTGACGCTGAATTGAAGTTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGTA
TTCAGCAAACGGAAGAAGAAGTGCCAGAAGGTGAAGAAGGTGCCGAAGTAGAAAAAGCACAGCTTGAAGCTCGACTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGACCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTGCTTCGCTCTATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGTTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATAATGATGAACGTATTCCGCGTGATCGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTGCTTCGTTCACCAATCGTTGGATTACATGATGAAGAACTTGCAACGCTTCG
TGCCCACGGAAAGAAAGGTTCGTTTTATGAAGTGATGAGCTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTACAAGGATGGCGTGAATTCGCACGTCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTCGGTGGTTTACCAGCCGGAAAGCAAAGACAGGCAAACTT
GCGTGTCTTATATGACCGCGCAAGACAATACGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGACGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGACGTCGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTTGAGTTCCCAGTCGTATTCGTAGCGGGGCTTGGTCGCCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGATTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCGTTAACGCGT
GCAAAAGAGAAGCTAATTTTAATTGGAACAGTGAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGTAGTATTCCGGCTGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAATACGTTACTTGCGCCAGAACCGGTTCAAGAAGAGAAACAAGAATTACTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGATGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGGGG
AAGCAGCATCTCACCGTGCGAAACAATCTGTTACGGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCGCCAATTCAAACACGCCCTCGTTTTATGGAGAAAAAAGGATTAACGTACGCTGAAAGAGG
TACGGCAGTTCACGCTGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGGGAACAAATTAATG
GCATGGTCAATAAAGAGCTATTAACATTCGAACAAGCAGAAGAAATAGCAATCGAAAAAGTCATTTCATTCTTTGACAGT
GATCTAGGTAAAAGAGTATTAGCGGCGAAAAGCGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGAAAGAGCGGGGAATCAATTCTTGTCCAAGGGGTTATTGACTGTATGATTGAAGAAGAAGACG
GTATTACGTTAATCGATTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGCGGATTTGATCAAGCGAAACCAATTTTA
GAAGATCGATATAAAGTGCAACTTTCTTTATATGCAAAGGCACTTGAAAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATATTTCTTTGATGGGAATCACGTTGTAAATATTGAAGAATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.586

100

0.536

Multiple sequence alignment