Detailed information NTG1094833

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: ACLBK2_RS05900
• Genome accession: NZ_CP180236
• Coordinates: 1131024..1134539 (+)
• Length: 1171 a.a.
• NCBI ID: WP_247998844.1
• Uniprot ID: -
• Organism: Bacillus sp. Ba 3
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1126024..1139539

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLBK2_RS05860 (ACLBK2_05860)	cspA	1126454..1126657 (+)	204	WP_000301519.1	RNA chaperone/antiterminator CspA	-
ACLBK2_RS05865 (ACLBK2_05865)	-	1126776..1126943 (+)	168	Protein_1058	IS5/IS1182 family transposase	-
ACLBK2_RS05870 (ACLBK2_05870)	-	1127028..1127246 (-)	219	WP_216475462.1	hypothetical protein	-
ACLBK2_RS05875 (ACLBK2_05875)	-	1127483..1127668 (-)	186	WP_216475463.1	hypothetical protein	-
ACLBK2_RS05880 (ACLBK2_05880)	-	1127953..1128534 (+)	582	WP_216475464.1	competence protein ComK	-
ACLBK2_RS05885 (ACLBK2_05885)	-	1128776..1129372 (+)	597	WP_071758313.1	DUF4306 domain-containing protein	-
ACLBK2_RS05890 (ACLBK2_05890)	-	1129694..1130287 (+)	594	WP_048526648.1	TVP38/TMEM64 family protein	-
ACLBK2_RS05895 (ACLBK2_05895)	lepB	1130344..1130907 (+)	564	WP_048526650.1	signal peptidase I	-
ACLBK2_RS05900 (ACLBK2_05900)	addB	1131024..1134539 (+)	3516	WP_247998844.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
ACLBK2_RS05905 (ACLBK2_05905)	addA	1134536..1138261 (+)	3726	WP_247998845.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
ACLBK2_RS05910 (ACLBK2_05910)	-	1138274..1138561 (+)	288	WP_000255723.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
ACLBK2_RS05915 (ACLBK2_05915)	-	1138655..1139032 (-)	378	WP_247998846.1	DUF1433 domain-containing protein	-

Sequence

Protein

• Length: 1171 a.a.
• Molecular weight: 134370.41 Da
• Isoelectric Point: 5.1127

>NTDB_id=1094833 ACLBK2_RS05900 WP_247998844.1 1131024..1134539(+) (addB) [Bacillus sp. Ba 3]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALISSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTLDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFYSQSLAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TYLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide

• Length: 3516 bp

>NTDB_id=1094833 ACLBK2_RS05900 WP_247998844.1 1131024..1134539(+) (addB) [Bacillus sp. Ba 3]
ATGTCACTTCGATTTGTAATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGTCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCATTAATTAGTA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCAGGCGTACATATGTTACTTCGTAAAATTGTAGAATCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTTTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTACA
ACGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTACTATATGATGATTTCGAGCGTGCTTTAATCGGAAAATATTTAGATTCAGAAGATTA
CTTGCAATTGCTAGTAGAAAAGCTTCCGCAGTCTGAATATGTAAATGGGGCTGAAATTTATATAGATGGATTTCATTCAT
TCTCACCGCAAGAACTAGAAATTGTAAGACAGCTTATGATTTGCGGTGCGAGAGTGACAATCACGTTAACACTAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACATATGAAAAAATAAAACAAGTAGC
ACGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCGCTTATGGAGCAACCGCGTTTTTATTCGCAATCATTAGCGCATT
TAGAAACGCATTACGAAGCTCGTCCAAATGAAAAGTTTCACGGAGAAGCGAGTGTAACGATTAGTACAGCAGCGAATTTA
CGAGCTGAAGTAGAAGGCGTTGCGCGTGAAATTCGTAGACTTGTGGCAGATGAAGACTATCGTTACCGAGATATCGCGGT
ACTTCTTCGTAACGGGGAAAGTTATTATGATGTAATGCGAACGCTATTTACAGATTATAATATCCCGCACTTCATTGATG
AAAAGCGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCTTATGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACGGACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGACTGAAGCGTGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTTGACGTTCCGAAAAAGCTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TCGCGACAGATCACGAGCAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTCGTAGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCCAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCGAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTCATTGGCGTAAATGAAGGAG
TTATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGACGTTCTTAGTGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
CATTTCTTGTCCGCTTGCAGATGAGGAAGGAAAGACGTTACTTGCTTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCCCGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTATCTTACGTTATGCAGCAACTTCAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAACTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGCTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGTTGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAACCGTTGCGCATACGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTTAAACTGGATGC
GCCAGATATCGGCGAATTATTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAATCGTACTTGGGCAG
ACTTATCAATAAAAGAATGCGAGCATCTTTCTGTTTTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGCCATTTCTATTTAAAACAAAAATTGCAGCAAATCATTTTCCGTACGTCAATCATTCTACGTGAACA
TGCGAAATCAAGTGGTTTCGTACCAGTAGATTTAGAAGTACCGTTCGGTATGGGCGGTACAGGATCGCTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACATACCTCCGTATTATTGATTATAAATCGAGCTCAAAAGCGTTAGATTTAACGGAAGTATACTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAAGGCGGCACGGCTTCACCGGCTGGTG
TACTGTACTTCCATATCCATAACCCAATCGTTGAGATGAAAGGTGACGCATCTGAAGCGGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTGCTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGATATTACAGAAGGCGTGATCGATATTGCCCCT
TATAAAAAAGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAATTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (795-1139)

• UvrD-like helicase C-terminal domain (UvrD_C) (303-669)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	49.061	100	0.491