Detailed information NTG1094064

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ACINK0_RS09745
• Genome accession: NZ_CP179945
• Coordinates: 1912517..1915180 (+)
• Length: 887 a.a.
• NCBI ID: WP_414656576.1
• Uniprot ID: -
• Organism: Deinococcus sp. VB343
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1907517..1920180

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACINK0_RS09725 (ACINK0_09725)	-	1908278..1910071 (-)	1794	WP_339096548.1	ABC transporter transmembrane domain-containing protein	-
ACINK0_RS09730 (ACINK0_09730)	-	1910190..1910987 (-)	798	WP_414656574.1	enoyl-ACP reductase	-
ACINK0_RS09735 (ACINK0_09735)	pgeF	1911102..1911863 (+)	762	WP_339096546.1	peptidoglycan editing factor PgeF	-
ACINK0_RS09740 (ACINK0_09740)	-	1911860..1912366 (+)	507	WP_414656575.1	YqeG family HAD IIIA-type phosphatase	-
ACINK0_RS09745 (ACINK0_09745)	pilB	1912517..1915180 (+)	2664	WP_414656576.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
ACINK0_RS09750 (ACINK0_09750)	pilT	1915231..1916523 (+)	1293	WP_339096541.1	type IV pilus twitching motility protein PilT	Machinery gene
ACINK0_RS09755 (ACINK0_09755)	-	1916680..1917165 (-)	486	WP_339096540.1	hypothetical protein	-
ACINK0_RS09760 (ACINK0_09760)	-	1917239..1917889 (-)	651	WP_339096539.1	ankyrin repeat domain-containing protein	-
ACINK0_RS09765 (ACINK0_09765)	-	1917973..1919172 (-)	1200	WP_339096538.1	acetyl-CoA C-acetyltransferase	-
ACINK0_RS09770 (ACINK0_09770)	-	1919182..1919766 (-)	585	WP_414656577.1	nucleotidyltransferase family protein	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 96945.87 Da
• Isoelectric Point: 4.8694

>NTDB_id=1094064 ACINK0_RS09745 WP_414656576.1 1912517..1915180(+) (pilB) [Deinococcus sp. VB343]
MALSIGDRRLGAILLEQGYVNDNDLQRALERHSEVGGRLADVLIDSGTVGEKRIARAIEEALGIPLVNLTAVEPEPEALH
AIRPQTALNLQAVPFGLEGDRLRVALVDPLSSFAIETLEDDSGLNIEPYQALRSEVMWALATFYPELGLEVVSPVSLSEA
KTGGKLGERLLAHGYITDAQLQVALDAQQQTGEALGSTLIAQRAITEEQLYEVLAEQERTTFLPNPSGFHPGEEVLGSML
RADALRLLSVPVDETDQGVVVLTSDPRKRADIEALIGRPVQMMLTRPRDIEALIEAFYPQRGRLGEALVNDGALSREQLR
EALQVQARGGKVKPLGEVISELGFASPDEIDSALAKQNTGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPVQNPPD
AKVALMIPEATARRYVVVPVRLQGNSLVVAMKDPRNVFALDDLKLITGKEILPAVMAEKDIIRLIERYFGEKGFDKLNKE
LADKAKNQQVQDTTDLAVADESAIVQVVDSIIREAALQEASDIHIETVEDAVKVRYRIDGSLREQSTFPKAAAQSIMARL
KIMGHLDIAERRVPQDGRIRFRKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPELEQLGFSEYNYPRYTEVIERPNGI
FLVTGPTGSGKSFTCFSTLKRIAKPEKNTTTIEDPIEYEVPGIVQSQVNNATGMTFARALRAFLRQDPDIIFVGEIRDQE
TAKIAVEAALTGHMVLATLHTNDAPGAVTRLEEMGVENFNISASVMGVLAQRLVRRVCSECKQPTNADPEVLRRLGISER
DIRGATLMRGAGCPRCGGTGYKGRMGIHELMVMDDHLRRAIGAGRPASELREVAVTESGMRTLRQDGIEKALQGLTTLEE
VLAVTAS

Nucleotide

• Length: 2664 bp

>NTDB_id=1094064 ACINK0_RS09745 WP_414656576.1 1912517..1915180(+) (pilB) [Deinococcus sp. VB343]
TTGGCTCTTTCGATTGGTGACCGTCGCCTCGGCGCGATTTTGCTGGAACAGGGGTACGTCAACGACAACGACTTGCAGCG
GGCGCTGGAAAGGCATTCTGAAGTCGGGGGTCGCCTTGCGGACGTGCTCATCGACTCCGGAACCGTCGGTGAAAAACGAA
TCGCCCGCGCCATCGAGGAAGCGCTGGGTATTCCGCTGGTCAACCTGACGGCGGTGGAGCCGGAACCCGAGGCGCTGCAT
GCCATCCGCCCCCAGACGGCGCTCAATCTTCAGGCGGTGCCGTTTGGGCTGGAGGGGGACCGCCTGCGGGTGGCGCTGGT
GGACCCACTGTCGAGCTTTGCCATCGAGACACTCGAAGACGACAGCGGTCTGAACATCGAACCGTATCAGGCGTTGCGGA
GCGAGGTGATGTGGGCGCTGGCGACCTTCTACCCCGAACTGGGGCTGGAAGTCGTGTCGCCCGTCAGCCTGTCGGAGGCC
AAAACCGGCGGCAAACTCGGTGAGCGCCTGCTGGCGCACGGCTACATCACCGACGCGCAGTTGCAGGTCGCGCTGGACGC
GCAGCAGCAGACGGGCGAGGCCCTGGGCAGCACCCTGATTGCCCAGCGGGCCATTACCGAGGAGCAGCTGTACGAGGTGC
TGGCCGAGCAGGAGCGGACCACCTTCTTGCCCAACCCCAGCGGCTTTCACCCCGGCGAGGAAGTGCTGGGCAGCATGCTC
CGCGCCGACGCCCTGCGGCTGCTCTCGGTACCGGTAGACGAAACCGACCAGGGCGTGGTCGTCCTGACCAGCGACCCGCG
CAAACGCGCCGACATCGAGGCGCTGATTGGCCGCCCGGTGCAGATGATGCTGACCCGCCCCCGCGACATCGAGGCGCTCA
TCGAGGCGTTCTACCCGCAGCGGGGCCGCCTGGGTGAAGCCCTGGTCAACGACGGTGCGCTCTCGCGCGAGCAGTTGCGC
GAAGCCCTGCAGGTGCAGGCGCGGGGCGGCAAGGTCAAGCCGCTGGGCGAGGTCATCAGCGAACTGGGCTTCGCCAGTCC
CGACGAAATCGACTCGGCGCTCGCCAAGCAGAACACGGGCGGCGGACGCCTGGAAGATACGCTGGTGCAGTCGGGCAAAC
TCAGCCCCGAGATGCTGGCCCGCTCGCTGGCCGCGCAGCTCGGTTACGAGTTCCTCGACCCGGTTCAGAACCCGCCCGAC
GCCAAGGTCGCCCTGATGATTCCCGAGGCCACCGCCCGACGCTACGTGGTGGTTCCGGTGCGTTTGCAGGGCAACTCGCT
CGTGGTCGCCATGAAAGACCCGCGCAACGTCTTTGCGCTCGACGACCTCAAGCTGATTACGGGCAAGGAGATCCTGCCCG
CGGTCATGGCGGAAAAGGACATCATCCGCCTGATCGAGCGCTACTTCGGGGAAAAGGGCTTCGACAAGCTCAATAAGGAA
CTCGCCGACAAGGCCAAGAACCAGCAGGTTCAGGACACCACCGACCTCGCGGTGGCCGACGAGAGCGCCATCGTGCAGGT
CGTGGACTCCATCATCCGTGAGGCGGCCCTGCAGGAAGCCTCGGATATCCATATCGAGACCGTTGAGGACGCCGTGAAGG
TGCGCTACCGCATCGACGGCTCCCTGCGCGAGCAGAGCACCTTCCCCAAGGCCGCCGCCCAGAGCATCATGGCCCGCCTC
AAGATCATGGGCCACCTCGACATCGCCGAGCGCCGGGTGCCGCAGGACGGGCGCATCCGCTTCCGCAAGGGCAGCATCGA
CCTCGACCTGCGTCTCTCGACCTTGCCCACCGTGTACGGCGAAAAGGCCGTCATGCGTCTGCTGCAAAAGGCCAGCAACA
TTCCCGAACTGGAGCAGCTCGGCTTTTCCGAGTACAACTACCCGCGCTACACCGAAGTCATCGAGCGGCCCAACGGCATT
TTCCTGGTGACCGGGCCGACGGGTTCGGGCAAGTCGTTCACCTGCTTTTCCACCCTCAAGCGCATCGCCAAGCCGGAAAA
GAACACCACGACCATCGAAGACCCCATCGAATACGAGGTGCCGGGCATCGTGCAGTCGCAGGTGAACAACGCCACCGGCA
TGACCTTTGCCCGCGCCCTGCGCGCCTTCTTGCGTCAGGACCCCGACATCATCTTCGTGGGGGAAATCCGTGACCAGGAA
ACCGCCAAAATCGCCGTGGAAGCCGCGCTGACCGGTCACATGGTGCTGGCGACCCTCCACACCAACGACGCGCCGGGGGC
CGTGACCCGCCTCGAAGAAATGGGCGTCGAGAACTTCAACATCTCGGCGTCGGTGATGGGCGTGCTGGCCCAACGCCTCG
TGCGCCGGGTATGCAGCGAGTGCAAGCAGCCCACCAACGCCGACCCCGAGGTGCTGCGCCGCCTGGGCATCAGCGAGCGC
GACATTCGCGGGGCGACCCTGATGCGCGGGGCGGGTTGCCCCCGCTGCGGCGGCACCGGCTACAAGGGCCGCATGGGGAT
TCACGAACTGATGGTGATGGACGACCACCTGCGCCGCGCCATCGGGGCCGGGCGGCCGGCGTCCGAACTGCGCGAAGTGG
CCGTGACCGAAAGCGGCATGCGAACCCTGCGGCAAGACGGCATCGAAAAAGCCCTCCAGGGGCTGACCACGCTCGAAGAA
GTGCTGGCAGTCACCGCGAGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (511-776)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-471)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (199-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	90.203	100	0.903
pilF	Thermus thermophilus HB27	56.067	100	0.563