Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   ACK25W_RS07570 Genome accession   NZ_CP179722
Coordinates   1512689..1513591 (+) Length   300 a.a.
NCBI ID   WP_106061506.1    Uniprot ID   -
Organism   Bacillus pumilus strain FJAT-56819     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1507689..1518591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK25W_RS07550 (ACK25W_07550) - 1508355..1510091 (+) 1737 WP_414051828.1 glycosyltransferase -
  ACK25W_RS07555 (ACK25W_07555) - 1510088..1510363 (+) 276 WP_003211418.1 FlhB-like flagellar biosynthesis protein -
  ACK25W_RS07560 (ACK25W_07560) sucC 1510540..1511700 (+) 1161 WP_012009945.1 ADP-forming succinate--CoA ligase subunit beta -
  ACK25W_RS07565 (ACK25W_07565) sucD 1511723..1512625 (+) 903 WP_003212080.1 succinate--CoA ligase subunit alpha -
  ACK25W_RS07570 (ACK25W_07570) dprA 1512689..1513591 (+) 903 WP_106061506.1 DNA-processing protein DprA Machinery gene
  ACK25W_RS07575 (ACK25W_07575) topA 1513785..1515860 (+) 2076 WP_060596250.1 type I DNA topoisomerase -
  ACK25W_RS07580 (ACK25W_07580) trmFO 1515931..1517235 (+) 1305 WP_034662383.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  ACK25W_RS07585 (ACK25W_07585) xerC 1517298..1518215 (+) 918 WP_060596252.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34067.32 Da        Isoelectric Point: 8.2776

>NTDB_id=1092848 ACK25W_RS07570 WP_106061506.1 1512689..1513591(+) (dprA) [Bacillus pumilus strain FJAT-56819]
MYNVSERMIFHRLKGLISPSLLTKWWKVDPELYINEETQHFKQDRSLQTIDFTRLKQAEENEFPIFQHIVQAYLKQNIHM
IPITSPLYPSTLKHIYDPPPVLFLKGNISYLNEEKSLGVVGTRVPSSYGEACVKKIVGELVKEDWTIVSGLAKGIDGLAH
KECIRNKGKTIGIIAGGFQHLYPKEHVQMAQYMGEHHLLLSEHPPYVKPEKWHFPMRNRLISALTSGTIVIQCKEKSGSL
ITAYQALEQGKEVFAVAGSIFDPNSTGPARLIQQGAKLVHSTKDILEEFSFHSVQYTELS

Nucleotide


Download         Length: 903 bp        

>NTDB_id=1092848 ACK25W_RS07570 WP_106061506.1 1512689..1513591(+) (dprA) [Bacillus pumilus strain FJAT-56819]
ATGTACAACGTGTCCGAAAGAATGATTTTTCACCGCTTAAAAGGCCTCATCTCACCCTCTTTGTTAACAAAATGGTGGAA
AGTCGATCCTGAGCTATATATAAATGAAGAAACACAACATTTCAAACAGGATCGATCATTACAAACGATCGACTTTACCC
GCTTAAAACAAGCCGAAGAAAATGAATTCCCCATTTTTCAACACATCGTTCAAGCCTATTTAAAGCAAAACATTCACATG
ATACCCATCACATCACCCTTATATCCCAGCACACTAAAACATATTTATGATCCTCCCCCTGTGTTATTCCTAAAAGGAAA
CATATCATATTTAAATGAAGAAAAAAGTTTAGGTGTAGTAGGCACACGAGTTCCATCGTCTTATGGAGAAGCATGTGTGA
AGAAAATTGTTGGTGAGCTTGTAAAGGAAGATTGGACGATTGTCAGTGGCTTAGCAAAAGGAATTGATGGACTTGCACAT
AAAGAGTGCATTCGGAATAAAGGGAAAACCATCGGTATTATAGCAGGCGGATTTCAGCACTTATACCCAAAGGAACATGT
GCAAATGGCTCAATACATGGGAGAGCATCATTTGCTTTTATCCGAGCATCCGCCTTATGTGAAACCAGAAAAGTGGCATT
TCCCAATGAGGAATCGTTTAATTAGTGCGCTGACAAGTGGAACCATCGTGATTCAGTGCAAAGAAAAGAGCGGTTCGCTC
ATTACCGCGTATCAAGCACTTGAGCAAGGTAAAGAGGTATTTGCGGTTGCAGGATCTATCTTTGATCCTAATTCCACAGG
ACCAGCCAGACTTATACAGCAGGGAGCAAAGCTTGTTCATTCAACGAAGGATATTTTAGAGGAATTCTCCTTCCACAGCG
TTCAATATACTGAACTCTCATAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

54.704

95.667

0.523
  dprA Legionella pneumophila strain ERS1305867

38.033

100

0.387
  dprA Lactococcus lactis subsp. cremoris KW2

38.408

96.333

0.37

Multiple sequence alignment