Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACL6EO_RS01735 Genome accession   NZ_CP178718
Coordinates   321213..321650 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain SY1154     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 316213..326650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL6EO_RS01710 (ACL6EO_01710) - 317233..318369 (+) 1137 WP_021494202.1 zinc-dependent alcohol dehydrogenase -
  ACL6EO_RS01715 (ACL6EO_01715) - 318384..318818 (+) 435 WP_021494203.1 RDD family protein -
  ACL6EO_RS01720 (ACL6EO_01720) - 318935..319213 (+) 279 WP_263612330.1 YckD family protein -
  ACL6EO_RS01725 (ACL6EO_01725) - 319317..320753 (+) 1437 WP_021494204.1 glycoside hydrolase family 1 protein -
  ACL6EO_RS01730 (ACL6EO_01730) nin/comJ 320794..321192 (-) 399 WP_014416873.1 competence protein ComJ Regulator
  ACL6EO_RS01735 (ACL6EO_01735) nucA/comI 321213..321650 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACL6EO_RS01740 (ACL6EO_01740) hxlB 321981..322538 (-) 558 WP_021494205.1 6-phospho-3-hexuloisomerase -
  ACL6EO_RS01745 (ACL6EO_01745) hxlA 322535..323170 (-) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  ACL6EO_RS01750 (ACL6EO_01750) - 323402..323764 (+) 363 WP_021494206.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1090894 ACL6EO_RS01735 WP_003156588.1 321213..321650(-) (nucA/comI) [Bacillus velezensis strain SY1154]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1090894 ACL6EO_RS01735 WP_003156588.1 321213..321650(-) (nucA/comI) [Bacillus velezensis strain SY1154]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment