Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACJQW4_RS07570 Genome accession   NZ_CP178606
Coordinates   1596600..1597364 (+) Length   254 a.a.
NCBI ID   WP_241627615.1    Uniprot ID   -
Organism   Acinetobacter sp. A7.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1591600..1602364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJQW4_RS07540 (ACJQW4_07540) - 1591754..1592314 (+) 561 WP_241627471.1 DUF2971 domain-containing protein -
  ACJQW4_RS07545 (ACJQW4_07545) - 1592473..1593756 (+) 1284 WP_241627473.1 glycosyltransferase family 4 protein -
  ACJQW4_RS07550 (ACJQW4_07550) - 1593785..1594336 (+) 552 WP_142767780.1 phosphatase PAP2 family protein -
  ACJQW4_RS07555 (ACJQW4_07555) iscX 1594417..1594617 (+) 201 WP_100242009.1 Fe-S cluster assembly protein IscX -
  ACJQW4_RS07560 (ACJQW4_07560) ndk 1594743..1595174 (+) 432 WP_016166212.1 nucleoside-diphosphate kinase -
  ACJQW4_RS07565 (ACJQW4_07565) rlmN 1595298..1596533 (+) 1236 WP_241627475.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACJQW4_RS07570 (ACJQW4_07570) pilF 1596600..1597364 (+) 765 WP_241627615.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACJQW4_RS07575 (ACJQW4_07575) - 1597355..1598149 (+) 795 WP_241627477.1 helix-turn-helix domain-containing protein -
  ACJQW4_RS07580 (ACJQW4_07580) ispG 1598170..1599285 (+) 1116 WP_161934347.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACJQW4_RS07585 (ACJQW4_07585) hisS 1599282..1600577 (+) 1296 WP_241627478.1 histidine--tRNA ligase -
  ACJQW4_RS07590 (ACJQW4_07590) - 1600611..1601315 (+) 705 WP_241627480.1 YfgM family protein -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 28621.60 Da        Isoelectric Point: 9.2865

>NTDB_id=1090412 ACJQW4_RS07570 WP_241627615.1 1596600..1597364(+) (pilF) [Acinetobacter sp. A7.4]
MSLVGCITTTTSSAPGFKKNPEKAVEVRTQLAAEYIRKGDLDAAKRALDQALEMEPRDSTANMMMGVLLQQEGSPANMEK
AEAHFKRAISADPKNAQARNNYGTYLYVVERYNEAIEQLTIAGSTLGYDQRFRALENLGRIYLKLGDTANAEKSFKQALQ
ANPNSYVSMLELSEIFYLRQQIPAATQLYEQFVRLVGQKNQSARALWIGIRIARANGDKMGMQVLVNQLRALFPDSQEYT
RYLQLQYSTEAVWK

Nucleotide


Download         Length: 765 bp        

>NTDB_id=1090412 ACJQW4_RS07570 WP_241627615.1 1596600..1597364(+) (pilF) [Acinetobacter sp. A7.4]
ATGTCGTTGGTGGGTTGTATTACAACAACCACCTCATCGGCACCAGGGTTTAAAAAAAATCCAGAGAAAGCAGTAGAAGT
TCGTACACAATTGGCAGCAGAATATATCCGTAAGGGCGACTTGGATGCAGCAAAGCGTGCACTTGATCAAGCATTGGAAA
TGGAACCACGAGATTCTACCGCCAATATGATGATGGGTGTGCTATTGCAGCAAGAAGGTAGTCCAGCCAATATGGAAAAA
GCGGAGGCACACTTTAAACGTGCCATTTCCGCAGATCCGAAAAATGCGCAAGCTCGTAACAACTATGGTACGTATTTATA
CGTCGTAGAACGTTATAATGAAGCAATTGAACAATTGACAATCGCTGGTTCAACCTTAGGGTATGATCAGCGATTTAGGG
CATTGGAAAATTTAGGGCGTATTTACTTAAAGTTAGGTGATACAGCAAATGCTGAAAAATCATTCAAACAAGCATTGCAA
GCAAATCCGAATTCCTATGTTTCAATGTTAGAGTTGTCGGAAATATTTTATTTAAGACAACAAATTCCTGCTGCGACACA
GTTATATGAACAATTTGTTCGTCTAGTTGGGCAGAAAAATCAGAGTGCACGTGCATTGTGGATTGGTATCCGTATTGCGC
GAGCCAATGGCGACAAGATGGGTATGCAAGTGTTGGTGAACCAACTACGTGCATTGTTCCCAGATAGCCAAGAATATACA
CGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(133-164)

Tetratricopeptide repeat (TPR_8)

(26-57)

Tetratricopeptide repeat (TPR_16)

(77-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78

98.425

0.768
  pilF Acinetobacter baylyi ADP1

71.825

99.213

0.713

Multiple sequence alignment