Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACJGE3_RS15930 Genome accession   NZ_CP178562
Coordinates   3215771..3216208 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain JT-3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3210771..3221208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJGE3_RS15890 (ACJGE3_15890) - 3211365..3211493 (-) 129 Protein_3093 hypothetical protein -
  ACJGE3_RS15895 (ACJGE3_15895) - 3211638..3212840 (+) 1203 WP_369614624.1 GTP-binding protein -
  ACJGE3_RS15900 (ACJGE3_15900) - 3212905..3214041 (+) 1137 WP_077721769.1 zinc-dependent alcohol dehydrogenase -
  ACJGE3_RS15905 (ACJGE3_15905) - 3214056..3214490 (+) 435 WP_369614625.1 RDD family protein -
  ACJGE3_RS15910 (ACJGE3_15910) - 3214608..3214883 (+) 276 WP_369719109.1 YckD family protein -
  ACJGE3_RS15915 (ACJGE3_15915) - 3214987..3215178 (+) 192 WP_071392145.1 family 1 glycosylhydrolase -
  ACJGE3_RS15920 (ACJGE3_15920) - 3215103..3215332 (+) 230 Protein_3099 family 1 glycosylhydrolase -
  ACJGE3_RS15925 (ACJGE3_15925) nin/comJ 3215351..3215749 (-) 399 WP_043866822.1 competence protein ComJ Regulator
  ACJGE3_RS15930 (ACJGE3_15930) nucA/comI 3215771..3216208 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACJGE3_RS15935 (ACJGE3_15935) hxlB 3216569..3217126 (-) 558 WP_077721772.1 6-phospho-3-hexuloisomerase -
  ACJGE3_RS15940 (ACJGE3_15940) hxlA 3217123..3217758 (-) 636 WP_369614626.1 3-hexulose-6-phosphate synthase -
  ACJGE3_RS15945 (ACJGE3_15945) - 3217990..3218352 (+) 363 WP_016936795.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1090198 ACJGE3_RS15930 WP_003156588.1 3215771..3216208(-) (nucA/comI) [Bacillus velezensis strain JT-3]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1090198 ACJGE3_RS15930 WP_003156588.1 3215771..3216208(-) (nucA/comI) [Bacillus velezensis strain JT-3]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment