Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA2   Type   Machinery gene
Locus tag   ACLWNE_RS04435 Genome accession   NZ_CP178485
Coordinates   840595..841155 (-) Length   186 a.a.
NCBI ID   WP_016329467.1    Uniprot ID   K7QX76
Organism   Thermus oshimai strain Blanco     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 835595..846155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLWNE_RS04405 (ACLWNE_04405) - 836196..836558 (-) 363 WP_413217562.1 nucleotidyltransferase family protein -
  ACLWNE_RS04410 (ACLWNE_04410) - 836622..837197 (+) 576 WP_413217563.1 Uma2 family endonuclease -
  ACLWNE_RS04415 (ACLWNE_04415) - 837194..837541 (-) 348 WP_413217564.1 type II secretion system protein -
  ACLWNE_RS04420 (ACLWNE_04420) - 837618..837974 (-) 357 WP_413217565.1 type II secretion system protein -
  ACLWNE_RS04425 (ACLWNE_04425) comZ 838209..839897 (-) 1689 WP_413217566.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  ACLWNE_RS04430 (ACLWNE_04430) pilA/pilA3 839906..840598 (-) 693 WP_339956364.1 PilW family protein Machinery gene
  ACLWNE_RS04435 (ACLWNE_04435) pilA/pilA2 840595..841155 (-) 561 WP_016329467.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACLWNE_RS04440 (ACLWNE_04440) - 841152..841619 (-) 468 WP_016329466.1 GspH/FimT family protein -
  ACLWNE_RS04445 (ACLWNE_04445) pilA/pilA1 841674..842144 (-) 471 WP_339956365.1 GspH/FimT family protein Machinery gene
  ACLWNE_RS04450 (ACLWNE_04450) - 842141..842515 (-) 375 WP_339956366.1 DUF1640 domain-containing protein -
  ACLWNE_RS04455 (ACLWNE_04455) - 842512..843270 (-) 759 WP_339956367.1 TerC family protein -
  ACLWNE_RS04460 (ACLWNE_04460) - 843295..844281 (+) 987 WP_339956368.1 YpdA family putative bacillithiol disulfide reductase -
  ACLWNE_RS04465 (ACLWNE_04465) ilvB 844427..846115 (+) 1689 WP_339956369.1 biosynthetic-type acetolactate synthase large subunit -

Sequence

Protein


Download         Length: 186 a.a.        Molecular weight: 20171.86 Da        Isoelectric Point: 8.4558

>NTDB_id=1089701 ACLWNE_RS04435 WP_016329467.1 840595..841155(-) (pilA/pilA2) [Thermus oshimai strain Blanco]
MRKGLTILEVLIALAVLGIAFAALLTSQLSSLRASGQARFASDTKAFAVQVLEDLSAQALKTEEKPSPDAYTDEERNGVY
RSFYFIDYYYRCPTAVTPPSGSGIRTALRTVTCSGQRTSPEGIRADWDYIGEGGRFGEGVLTVVVTATHPRGPRVTVGRR
ITCYDVYPSPTHDKPRPCPEPGGGRP

Nucleotide


Download         Length: 561 bp        

>NTDB_id=1089701 ACLWNE_RS04435 WP_016329467.1 840595..841155(-) (pilA/pilA2) [Thermus oshimai strain Blanco]
GTGAGAAAAGGCCTCACCATTCTGGAAGTCCTCATCGCCCTGGCCGTCTTGGGCATCGCCTTCGCCGCGCTCCTCACCAG
CCAGCTTTCCAGCCTGAGGGCCTCGGGCCAGGCCCGCTTCGCCTCCGACACCAAGGCCTTCGCCGTGCAGGTGCTGGAAG
ACCTTTCCGCCCAAGCCCTGAAGACGGAGGAGAAGCCCTCCCCAGATGCCTACACCGACGAGGAGCGGAACGGGGTCTAC
CGGAGCTTCTACTTCATCGACTACTACTACCGCTGCCCCACCGCCGTAACCCCCCCTTCGGGCTCGGGCATCCGCACCGC
CTTACGGACCGTCACCTGCAGCGGGCAGAGGACGAGCCCCGAGGGCATCCGGGCTGACTGGGACTACATCGGCGAGGGTG
GGCGCTTTGGGGAAGGGGTGCTCACGGTGGTGGTCACCGCCACCCACCCCCGGGGGCCTAGGGTCACCGTGGGGCGGCGG
ATCACCTGCTACGACGTCTACCCCTCCCCCACCCACGACAAGCCCCGCCCCTGCCCCGAACCGGGAGGTGGAAGGCCATG
A

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K7QX76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA2 Thermus thermophilus HB27

58.791

97.849

0.575

Multiple sequence alignment