Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   ACK3BE_RS27140 Genome accession   NZ_CP177237
Coordinates   5925105..5926445 (+) Length   446 a.a.
NCBI ID   WP_010467552.1    Uniprot ID   A0A024EFS0
Organism   Pseudomonas mandelii strain VR4' HT30-3     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5920105..5931445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK3BE_RS27115 (ACK3BE_27115) pgeF 5920324..5921043 (-) 720 WP_221107414.1 peptidoglycan editing factor PgeF -
  ACK3BE_RS27120 (ACK3BE_27120) rluD 5921040..5922002 (-) 963 WP_007942311.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  ACK3BE_RS27125 (ACK3BE_27125) - 5922154..5923176 (+) 1023 WP_010467556.1 outer membrane protein assembly factor BamD -
  ACK3BE_RS27130 (ACK3BE_27130) - 5923290..5923523 (+) 234 WP_094470587.1 PP0621 family protein -
  ACK3BE_RS27135 (ACK3BE_27135) pilS 5923513..5925102 (+) 1590 WP_010467553.1 sensor histidine kinase Regulator
  ACK3BE_RS27140 (ACK3BE_27140) pilR 5925105..5926445 (+) 1341 WP_010467552.1 sigma-54-dependent transcriptional regulator Regulator
  ACK3BE_RS27145 (ACK3BE_27145) thiO 5926575..5927675 (-) 1101 WP_010467551.1 glycine oxidase ThiO -
  ACK3BE_RS27150 (ACK3BE_27150) - 5927734..5928135 (-) 402 WP_083375726.1 type IV pilin protein -
  ACK3BE_RS27155 (ACK3BE_27155) - 5928150..5931260 (-) 3111 WP_304873827.1 pilus assembly protein -

Sequence

Protein


Download         Length: 446 a.a.        Molecular weight: 49813.28 Da        Isoelectric Point: 6.4102

>NTDB_id=1086342 ACK3BE_RS27140 WP_010467552.1 5925105..5926445(+) (pilR) [Pseudomonas mandelii strain VR4' HT30-3]
MSPRPKVLIVDDEPDIRELLEITLGRMKLDTYSARNLGEAQALLMRETFDLCLTDMRLPDGTGLELVQYIQQRYPHVPVA
MITAYGSLETAINALKAGAFDFLTKPVDLTRLRELVTSALRLPAPGGASTAIDRRLLGDSLPMRNLRKQIDKLARSQAPV
YISGESGSGKELVARLIHEQGPRANQPFVPVNCGAIPSELMESEFFGHRKGSFSGAIEDKPGLFQAAHGGTLFLDEVADL
PLAMQVKLLRAIQEKAIRSVGGQQEMVVDVRILCATHKDLDAEVTAERFRQDLYYRLNVIELRVPPLRERRDDIEPLASH
VLKRLAAGSGQPAAKLHPLALDALKSYRFPGNVRELENVLERAYTLCEDKQIEASDLRLAEGNCTAESGVPDLTQIDNLE
DYLENVERKLILQALEETRWNRTAAAQRLNLSFRSMRYRLKKLGLD

Nucleotide


Download         Length: 1341 bp        

>NTDB_id=1086342 ACK3BE_RS27140 WP_010467552.1 5925105..5926445(+) (pilR) [Pseudomonas mandelii strain VR4' HT30-3]
ATGAGCCCACGGCCAAAAGTCCTGATCGTCGACGACGAACCGGATATCCGCGAACTACTGGAAATCACCCTGGGACGGAT
GAAACTCGATACCTACAGTGCCCGCAACCTTGGCGAAGCCCAAGCCTTGTTGATGCGCGAGACATTCGACCTGTGCCTGA
CCGACATGCGCCTGCCCGACGGCACCGGCCTTGAGTTGGTGCAGTACATCCAGCAACGTTACCCACACGTGCCGGTGGCG
ATGATCACCGCTTACGGCAGCCTCGAAACAGCGATCAACGCCCTCAAGGCAGGGGCTTTCGACTTCCTGACCAAGCCGGT
TGACCTGACGCGCCTGCGCGAACTGGTCACCAGCGCCCTGCGCTTGCCGGCACCGGGTGGCGCCAGTACCGCCATCGATC
GCCGGTTGCTGGGCGATTCACTGCCCATGCGCAACCTGCGCAAACAAATCGACAAACTCGCACGCAGTCAGGCTCCGGTG
TACATCAGCGGCGAATCCGGCAGTGGCAAGGAATTGGTCGCCCGACTGATCCACGAGCAGGGCCCGCGGGCTAATCAACC
GTTCGTCCCGGTCAATTGCGGGGCAATTCCGTCAGAGTTGATGGAGAGTGAATTCTTCGGCCATCGCAAAGGCAGTTTCA
GCGGCGCCATCGAAGACAAACCAGGGCTGTTCCAGGCCGCCCACGGCGGCACACTGTTTCTCGACGAAGTGGCCGATCTG
CCCTTGGCGATGCAGGTCAAACTGCTCCGAGCCATTCAGGAGAAAGCCATTCGCAGTGTCGGCGGTCAGCAAGAAATGGT
GGTTGATGTCCGCATCCTTTGTGCCACCCACAAAGACCTCGATGCCGAAGTCACCGCCGAACGCTTTCGCCAGGATTTGT
ACTACCGGTTGAACGTGATCGAACTGCGCGTTCCCCCTTTGCGCGAGCGCCGCGACGACATCGAACCGCTGGCCAGCCAT
GTGCTCAAGCGCCTGGCGGCCGGCAGCGGTCAACCCGCCGCAAAACTCCATCCACTCGCCCTGGACGCACTGAAAAGTTA
CCGTTTTCCGGGCAACGTGCGGGAGCTGGAGAACGTGCTGGAGCGGGCTTACACCCTGTGCGAGGACAAACAGATTGAAG
CCAGCGACCTGCGACTGGCCGAAGGTAACTGCACGGCAGAGAGCGGCGTGCCGGACCTGACGCAAATCGATAACCTGGAA
GATTATCTGGAAAACGTCGAACGCAAACTCATCCTCCAGGCACTGGAGGAAACCCGCTGGAACCGCACGGCGGCGGCTCA
GCGGTTGAATCTTTCATTCAGATCGATGCGATATCGGCTCAAGAAATTGGGTCTGGATTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-116)

Sigma-54 interaction domain (Sigma54_activat)

(137-302)

Bacterial regulatory protein, Fis family (HTH_8)

(403-443)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A024EFS0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

79.458

99.327

0.789
  pilR Acinetobacter baumannii strain A118

49.46

100

0.513

Multiple sequence alignment