Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ACLB6K_RS15885 Genome accession   NZ_CP177167
Coordinates   3262853..3264868 (+) Length   671 a.a.
NCBI ID   WP_123231222.1    Uniprot ID   -
Organism   Microcystis aeruginosa FACHB-524     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3257853..3269868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLB6K_RS15855 (ACLB6K_15850) - 3258096..3258491 (-) 396 WP_002763099.1 PH domain-containing protein -
  ACLB6K_RS15860 (ACLB6K_15855) rnpA 3258481..3258843 (-) 363 WP_002738574.1 ribonuclease P protein component -
  ACLB6K_RS15865 (ACLB6K_15860) rpmH 3258870..3259007 (-) 138 WP_002739276.1 50S ribosomal protein L34 -
  ACLB6K_RS15870 (ACLB6K_15865) - 3259099..3261063 (+) 1965 WP_123231220.1 amylo-alpha-1,6-glucosidase -
  ACLB6K_RS15875 (ACLB6K_15870) - 3261154..3261795 (+) 642 WP_123231221.1 HAD-IA family hydrolase -
  ACLB6K_RS15880 (ACLB6K_15875) grpE 3261890..3262612 (-) 723 WP_002738986.1 nucleotide exchange factor GrpE -
  ACLB6K_RS15885 (ACLB6K_15880) pilB/pilB1 3262853..3264868 (+) 2016 WP_123231222.1 GspE/PulE family protein Machinery gene
  ACLB6K_RS15890 (ACLB6K_15885) pilT 3265014..3266120 (+) 1107 WP_123231223.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLB6K_RS15895 (ACLB6K_15890) - 3266262..3267476 (+) 1215 WP_002778013.1 type II secretion system F family protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 75093.29 Da        Isoelectric Point: 6.4268

>NTDB_id=1085763 ACLB6K_RS15885 WP_123231222.1 3262853..3264868(+) (pilB/pilB1) [Microcystis aeruginosa FACHB-524]
MTFSSKKTRAVALRNYFSPFGNKLVQSGYIGAEQMQQALVETRKSGRSLVEILQKLTGRPLPPDLQRQYKKNQLFELKIL
YGVESLDPELSDVDGLEIARLIDSLIPIDLCRRYRLIPLRRIEGEPAAILIAMVDPDNLAATDDINRILRPRELGLQRLV
IAGEDYERLLEKFHSAQSELEREKARLQKEKELEKLSDLTDIVGSLDLTSGVANDEVADDLGEGDANQAPVINLVNKIIA
KALQEGSSDIHIEPQEEFLKIRFRKDGVLHQAFEPLPKKIAPAVTARFKIMADLDISERRLPQDGKIRRMYQGRKVDFRV
NTLPSRYGEKVCLRILDNSATQLGLDKLITDRNTLQIVRDMASRPFGLLLVTGPTGSGKSTTLYSVLAERNHPGVNISTA
EDPIEYSLPGITQVQIIREKGMDFASILRAFLRQDPDVILVGETRDRETAKTVIEAALTGHLVLTTLHTNDAAGAIVRLD
KMGIEPFMISGSLLGILAQRLVRRVCTQCRVAYHPSQEELARFGLSAANEQEVTFYKANTLTIEEIQQARKQGNLCLKCA
GSGYKDRIGVYEVMQNSERIQQLINQGATTDRIKEAAVDEGMVTLLAYSLNLVREGYTTLEEVERVTLTDSGLEAELKAK
RKSGLICSGCRAELQPEWLDCPYCMTPRFSY

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=1085763 ACLB6K_RS15885 WP_123231222.1 3262853..3264868(+) (pilB/pilB1) [Microcystis aeruginosa FACHB-524]
ATGACATTTTCTTCCAAGAAAACCCGTGCTGTCGCCCTACGCAATTACTTTTCCCCCTTTGGTAACAAGTTAGTGCAGTC
GGGCTATATCGGTGCCGAGCAAATGCAACAGGCCCTGGTAGAAACGCGGAAATCGGGACGATCGCTAGTAGAAATATTGC
AAAAACTAACCGGGCGGCCTTTGCCCCCGGATTTACAGCGACAGTATAAGAAAAACCAGTTATTTGAACTAAAAATCCTC
TACGGAGTCGAATCGCTCGACCCAGAATTGAGCGATGTGGATGGACTGGAAATAGCCAGGCTGATCGATTCTTTGATTCC
GATCGATCTTTGTCGTCGCTATCGTCTCATCCCCCTGCGTCGAATCGAAGGAGAGCCAGCCGCGATTTTGATAGCCATGG
TCGATCCCGACAACCTCGCGGCTACTGACGATATAAATCGTATCCTCCGCCCCAGGGAGTTAGGTTTACAGCGTTTGGTG
ATTGCCGGGGAAGATTATGAAAGATTACTGGAAAAATTCCACTCGGCTCAATCGGAACTAGAACGGGAGAAAGCCCGACT
GCAAAAAGAAAAAGAACTGGAAAAACTCTCGGATCTGACCGATATTGTCGGCAGTCTTGATTTGACCTCTGGTGTTGCTA
ACGATGAAGTGGCCGATGATCTCGGAGAAGGGGATGCTAATCAAGCCCCCGTCATCAACCTTGTCAATAAAATTATAGCT
AAAGCTCTACAGGAAGGATCCTCCGATATTCACATCGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAAAGATGG
AGTTCTTCATCAAGCTTTTGAGCCACTGCCGAAAAAAATTGCCCCGGCGGTCACAGCCCGGTTTAAAATCATGGCCGATC
TCGATATTTCCGAACGTCGTCTTCCCCAGGATGGTAAAATTCGGCGGATGTATCAAGGACGAAAAGTAGATTTTCGGGTT
AATACCCTGCCCAGTCGCTACGGGGAAAAAGTCTGTTTAAGGATTCTCGATAACTCGGCTACCCAGTTGGGTTTAGATAA
ACTAATCACCGATCGAAATACTCTGCAAATCGTCCGAGACATGGCTAGTCGTCCCTTTGGTCTGTTGCTGGTGACAGGCC
CCACCGGTTCAGGGAAATCCACCACTTTATACTCGGTGTTAGCGGAAAGAAATCACCCTGGGGTCAATATTAGTACGGCC
GAAGACCCGATCGAATATTCTCTGCCAGGCATCACCCAGGTGCAGATAATTCGCGAAAAAGGCATGGATTTCGCCTCGAT
TCTGCGGGCATTCCTGCGACAAGACCCGGATGTGATTCTGGTGGGAGAAACTAGGGATAGGGAAACGGCTAAAACGGTGA
TCGAGGCCGCCTTAACCGGTCACTTGGTCTTAACTACCCTACACACCAACGATGCCGCAGGTGCGATCGTTCGTCTTGAT
AAGATGGGGATTGAACCGTTCATGATTTCTGGTTCTCTGTTGGGAATCTTGGCCCAGCGTCTCGTGCGGCGCGTCTGTAC
CCAGTGTCGGGTCGCCTATCACCCTAGTCAAGAGGAATTAGCCCGTTTTGGTCTTTCCGCTGCCAATGAACAGGAAGTGA
CTTTTTATAAGGCTAATACCCTAACTATAGAGGAAATTCAACAAGCGCGAAAACAGGGCAATCTCTGCCTTAAGTGTGCT
GGCAGCGGTTACAAAGATCGCATCGGTGTCTATGAGGTAATGCAGAATAGTGAACGAATACAGCAGTTAATTAACCAAGG
GGCGACAACCGATCGCATTAAGGAGGCGGCCGTTGATGAGGGCATGGTGACACTCCTAGCCTATAGTTTAAATCTGGTGC
GCGAGGGTTACACCACCCTCGAAGAAGTAGAACGGGTGACACTGACCGATTCTGGTCTAGAAGCGGAATTAAAAGCCAAA
CGGAAAAGCGGTTTAATCTGTAGTGGTTGTCGCGCTGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGACACC
GCGATTTAGTTATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-179)

Type II/IV secretion system protein (T2SSE)

(237-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

72.205

98.659

0.712

Multiple sequence alignment