Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AACH39_RS15725 Genome accession   NZ_AP029060
Coordinates   3310527..3311954 (-) Length   475 a.a.
NCBI ID   WP_215777744.1    Uniprot ID   -
Organism   Paludibacterium sp. THUN1379     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3305527..3316954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH39_RS15695 (THUN1379_30660) - 3305665..3307152 (-) 1488 WP_338620545.1 NAD(P)H-hydrate dehydratase -
  AACH39_RS15700 (THUN1379_30670) - 3307149..3307766 (-) 618 WP_215777757.1 flavin prenyltransferase UbiX -
  AACH39_RS15705 (THUN1379_30680) rapZ 3307806..3308651 (-) 846 WP_215777754.1 RNase adapter RapZ -
  AACH39_RS15710 (THUN1379_30690) hprK 3308632..3309588 (-) 957 WP_215777751.1 HPr(Ser) kinase/phosphatase -
  AACH39_RS15715 (THUN1379_30700) ptsN 3309581..3310042 (-) 462 WP_215777748.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AACH39_RS15720 (THUN1379_30710) raiA 3310143..3310457 (-) 315 WP_215777746.1 ribosome-associated translation inhibitor RaiA -
  AACH39_RS15725 (THUN1379_30720) rpoN/rpoN1 3310527..3311954 (-) 1428 WP_215777744.1 RNA polymerase factor sigma-54 Machinery gene
  AACH39_RS15730 (THUN1379_30730) lptB 3311961..3312689 (-) 729 WP_215777742.1 LPS export ABC transporter ATP-binding protein -
  AACH39_RS15735 (THUN1379_30740) lptA 3312692..3313231 (-) 540 WP_215777739.1 lipopolysaccharide transport periplasmic protein LptA -
  AACH39_RS15740 (THUN1379_30750) lptC 3313221..3313787 (-) 567 WP_338620546.1 LPS export ABC transporter periplasmic protein LptC -
  AACH39_RS15745 (THUN1379_30760) - 3313784..3314308 (-) 525 WP_215777734.1 HAD family hydrolase -
  AACH39_RS15750 (THUN1379_30770) - 3314335..3315315 (-) 981 WP_215777732.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 52976.85 Da        Isoelectric Point: 4.7096

>NTDB_id=108355 AACH39_RS15725 WP_215777744.1 3310527..3311954(-) (rpoN/rpoN1) [Paludibacterium sp. THUN1379]
MKQSLQLKVSQQLTLTPQLQQSIKLLQLSTVELQAEVERYLLENPLLERDEDTPGEPGLPERPMESSHSEEVPKQQEQDT
ASEPGMEALQDWGSGSGTGSGGSYDDEFDPFYNVPCHQTLRELLLSQLGEIDLPQRDHAVVQLLIEELDDDGYLTTPLAE
LVASLPEELALDEDELAVGLRMLQQFDPPGVGARSLPESLQLQLARLPASTPGKSLAGDIIAQHLALLGARDYTRLRKLL
GTSDDALREAQTLIARLNPRPASGFDARDTHYVTPDVTVRKRKGRWVAELNSSAVPQLRVNQIYARMLSENRQQSGDLGS
RLQEARWLVKNIQQRFDTILKVSEAIVDKQQAFFEQGEVAMRPMILRNIADELGLHESTVSRVTTQKYLLCPRGLFELKY
FFGSALETDSGEECSATAIKAHIRRLIEQENPSRPLSDSAIAEQLSSQGIQVARRTVAKYREAMQIAPVNQRKAL

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=108355 AACH39_RS15725 WP_215777744.1 3310527..3311954(-) (rpoN/rpoN1) [Paludibacterium sp. THUN1379]
ATGAAGCAAAGCCTCCAGCTCAAAGTCTCCCAGCAGTTGACCCTGACGCCGCAATTGCAGCAGTCGATCAAGCTGCTGCA
ACTGTCGACGGTCGAACTGCAAGCCGAAGTCGAACGCTATCTGCTGGAAAACCCGCTGCTCGAGCGCGATGAAGACACTC
CCGGCGAACCCGGCCTGCCGGAGCGCCCGATGGAAAGCAGCCACAGCGAAGAGGTCCCGAAGCAGCAGGAGCAGGACACG
GCGAGCGAACCGGGCATGGAAGCGCTGCAGGACTGGGGCAGCGGCAGCGGGACCGGCAGTGGTGGCAGCTACGACGACGA
ATTCGACCCCTTTTACAATGTGCCCTGTCATCAGACCCTGCGCGAGCTGTTGCTGTCGCAGCTGGGAGAAATTGACCTGC
CGCAGCGCGACCACGCCGTCGTGCAACTGCTGATTGAAGAACTGGATGACGATGGCTACCTGACCACGCCGCTGGCCGAA
CTGGTGGCCAGTCTGCCCGAAGAGCTGGCCCTGGACGAAGATGAACTGGCCGTCGGCCTGCGCATGCTGCAGCAGTTCGA
CCCCCCCGGCGTCGGCGCCCGCTCACTGCCCGAATCCCTGCAACTGCAACTGGCCAGATTACCGGCCAGCACCCCCGGCA
AATCCCTGGCCGGCGACATCATCGCGCAGCACCTGGCCCTGCTCGGCGCCCGTGACTACACCCGCCTGCGCAAGCTGCTG
GGCACCAGCGACGATGCCCTGCGCGAAGCCCAGACCCTGATCGCCCGCCTCAACCCGCGTCCGGCCAGCGGCTTCGACGC
GCGCGACACCCATTACGTCACCCCGGACGTCACCGTCAGAAAGCGTAAAGGCCGCTGGGTGGCCGAGCTCAACAGCAGCG
CCGTCCCGCAATTGCGCGTCAACCAGATCTACGCCCGCATGCTGTCGGAAAACCGCCAGCAATCCGGCGATCTCGGCAGT
CGTCTGCAAGAGGCACGGTGGCTGGTCAAGAACATTCAACAGCGATTTGACACTATCCTGAAAGTGTCGGAAGCTATAGT
CGACAAGCAGCAAGCGTTCTTTGAACAAGGCGAAGTGGCCATGCGGCCGATGATCCTGCGCAATATCGCCGATGAGCTCG
GGCTGCACGAATCCACCGTCTCCAGAGTCACCACCCAGAAATATCTGCTCTGTCCCCGGGGACTTTTCGAGCTGAAATAT
TTCTTCGGCAGTGCCCTGGAAACCGACAGTGGCGAAGAATGCTCCGCCACCGCCATCAAGGCACATATCCGCAGGCTGAT
CGAACAGGAGAACCCATCCAGGCCACTGTCAGACAGCGCCATTGCCGAACAGCTATCCAGCCAGGGAATTCAGGTTGCAA
GACGTACCGTTGCCAAGTATCGTGAAGCCATGCAGATAGCGCCGGTCAATCAAAGAAAGGCTCTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(117-304)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-473)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.594

100

0.545

Multiple sequence alignment