Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   ACK8N4_RS26060 Genome accession   NZ_CP176811
Coordinates   5549391..5550983 (+) Length   530 a.a.
NCBI ID   WP_039028118.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain DG-1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5544391..5555983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8N4_RS26040 (ACK8N4_26040) pgeF 5546141..5546869 (-) 729 WP_012614543.1 peptidoglycan editing factor PgeF -
  ACK8N4_RS26045 (ACK8N4_26045) rluD 5546866..5547828 (-) 963 WP_033972915.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  ACK8N4_RS26050 (ACK8N4_26050) - 5547974..5548999 (+) 1026 WP_033972916.1 outer membrane protein assembly factor BamD -
  ACK8N4_RS26055 (ACK8N4_26055) - 5549156..5549401 (+) 246 WP_003102591.1 PP0621 family protein -
  ACK8N4_RS26060 (ACK8N4_26060) pilS 5549391..5550983 (+) 1593 WP_039028118.1 two-component system sensor histidine kinase PilS Regulator
  ACK8N4_RS26065 (ACK8N4_26065) pilR 5550998..5552335 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  ACK8N4_RS26070 (ACK8N4_26070) thiO 5552381..5553475 (-) 1095 WP_083557131.1 glycine oxidase ThiO -
  ACK8N4_RS26075 (ACK8N4_26075) - 5553610..5554119 (+) 510 WP_083557114.1 Tfp pilus assembly protein FimT/FimU -
  ACK8N4_RS26080 (ACK8N4_26080) - 5554225..5554731 (+) 507 WP_003134935.1 Tfp pilus assembly protein FimT/FimU -
  ACK8N4_RS26085 (ACK8N4_26085) pilV 5554722..5555279 (+) 558 WP_003125211.1 type 4a pilus minor pilin PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59116.28 Da        Isoelectric Point: 6.4439

>NTDB_id=1083229 ACK8N4_RS26060 WP_039028118.1 5549391..5550983(+) (pilS) [Pseudomonas aeruginosa strain DG-1]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVLPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SELHLQLGADDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLVRDPESDLPVLEVIDDGPGVPTDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=1083229 ACK8N4_RS26060 WP_039028118.1 5549391..5550983(+) (pilS) [Pseudomonas aeruginosa strain DG-1]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
AGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAATATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCAGCGGCCAGCCTCG
GGCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGCCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTACGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAAGAACTCAACGCATTGATCCTGCAGCGTATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTACAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCACCGACGCTCAAGGTGCT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAA
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCTGCCCAGCTGCTGCAGGAGTCCGAGGAGCTGGACGCCCCGGACCGGCG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAT
AGCGAACTGCACCTGCAACTCGGTGCCGACGACATCCAGACCCGCATGGACCCACACCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGTGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGACGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAGCTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.057

100

0.991

Multiple sequence alignment