Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACK3B6_RS12670 Genome accession   NZ_CP176792
Coordinates   2505309..2506379 (-) Length   356 a.a.
NCBI ID   WP_095713275.1    Uniprot ID   -
Organism   Bacillus halotolerans strain BCP32     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2500309..2511379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK3B6_RS12625 (ACK3B6_12625) tapA 2500784..2501545 (-) 762 WP_105955621.1 amyloid fiber anchoring/assembly protein TapA -
  ACK3B6_RS12630 (ACK3B6_12630) - 2501822..2502145 (+) 324 WP_024122040.1 YqzG/YhdC family protein -
  ACK3B6_RS12635 (ACK3B6_12635) - 2502188..2502367 (-) 180 WP_003236949.1 YqzE family protein -
  ACK3B6_RS12640 (ACK3B6_12640) comGG 2502439..2502813 (-) 375 WP_101864528.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACK3B6_RS12645 (ACK3B6_12645) comGF 2502814..2503197 (-) 384 WP_181185692.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACK3B6_RS12650 (ACK3B6_12650) comGE 2503223..2503570 (-) 348 WP_105955577.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACK3B6_RS12655 (ACK3B6_12655) comGD 2503554..2503985 (-) 432 WP_105955576.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACK3B6_RS12660 (ACK3B6_12660) comGC 2503975..2504271 (-) 297 WP_010334925.1 competence type IV pilus major pilin ComGC Machinery gene
  ACK3B6_RS12665 (ACK3B6_12665) comGB 2504285..2505322 (-) 1038 WP_202853060.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACK3B6_RS12670 (ACK3B6_12670) comGA 2505309..2506379 (-) 1071 WP_095713275.1 competence protein ComGA Machinery gene
  ACK3B6_RS12675 (ACK3B6_12675) - 2506702..2507112 (-) 411 WP_105955574.1 CBS domain-containing protein -
  ACK3B6_RS12680 (ACK3B6_12680) - 2507175..2508128 (-) 954 WP_105991987.1 magnesium transporter CorA family protein -
  ACK3B6_RS12685 (ACK3B6_12685) - 2508272..2509601 (+) 1330 Protein_2449 hemolysin family protein -
  ACK3B6_RS12690 (ACK3B6_12690) - 2509681..2510520 (-) 840 WP_044154573.1 STAS domain-containing protein -
  ACK3B6_RS12700 (ACK3B6_12700) mgsR 2510753..2511133 (-) 381 WP_026014741.1 transcriptional regulator MgsR -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40480.69 Da        Isoelectric Point: 8.8394

>NTDB_id=1083008 ACK3B6_RS12670 WP_095713275.1 2505309..2506379(-) (comGA) [Bacillus halotolerans strain BCP32]
MDSIEKISKTLIEEAYRTKASDIHIVPRERDAIIHFRVDHALLKKRNMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGDVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLIQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSTGLKAILRHDPDMIILGEIRDAETAQIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCEDGCSSVYCRLSRNVRRASVYELLYGKNLQQCIQEAKGD
NANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHETD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1083008 ACK3B6_RS12670 WP_095713275.1 2505309..2506379(-) (comGA) [Bacillus halotolerans strain BCP32]
TTGGATTCGATAGAAAAAATAAGCAAAACTTTAATTGAAGAGGCATATAGGACGAAGGCCTCTGACATTCACATTGTGCC
GAGGGAACGGGACGCAATCATTCATTTTCGGGTTGACCATGCATTGCTGAAAAAGCGAAACATGAAAAAAGAAGAGTGTG
TAAGGCTCATTTCTCATTTTAAGTTTCTCTCAGCTATGGACATAGGGGAAAGAAGAAAGCCGCAAAATGGATCTCTTACT
CTAAAATTAAAAGAGGGTGACGTTCACTTAAGGATGTCAACGCTCCCGACAATTAACGAAGAAAGCCTCGTCATCAGAGT
GATGCCTCAGTATAATATCCCTTCGATTGACAAACTGTCTTTATTCCCAAAGACAGGAGCCACCTTGCTATCATTTTTAA
AACACTCCCACGGCATGCTGATTTTTACCGGGCCAACAGGCTCTGGGAAAACAACAACATTATATTCTCTGATCCAATAT
GCAAAAAAACATTTTAATCGAAACATTGTCACACTTGAAGATCCAGTGGAAACGAGGGATGAAGATGTTCTTCAAGTGCA
GGTGAATGAAAAAGCCGGTGTCACATATTCCACAGGACTGAAGGCTATTTTGCGCCATGACCCCGATATGATCATTTTAG
GTGAGATTAGAGACGCTGAAACAGCCCAGATCGCGGTGCGGGCAGCGATGACTGGTCATTTAGTGCTGACGAGCCTTCAT
ACGAGAGATGCGAAGGGCGCGATTTACAGGCTGCTTGAATTTGGCATTAATATGAATGAAATCGAGCAAACGGTTATTGC
GATAGCGGCTCAGCGTTTAGTTGATTTGGCTTGCCCGTTTTGTGAAGACGGATGTTCATCAGTGTATTGCCGCCTGTCAC
GAAATGTCAGGAGAGCAAGCGTATACGAGCTGCTTTACGGGAAAAACCTTCAGCAGTGTATCCAGGAAGCGAAAGGGGAC
AATGCAAATTACCAATATCAAACGCTGCGTCAAATTATAAGAAAAGGGATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAACAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

96.067

100

0.961

Multiple sequence alignment