Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACK1LH_RS14255 Genome accession   NZ_CP176757
Coordinates   2770494..2771561 (-) Length   355 a.a.
NCBI ID   WP_029279705.1    Uniprot ID   -
Organism   Metabacillus indicus strain JKLC.Or24     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2765494..2776561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK1LH_RS14200 (ACK1LH_14200) - 2765613..2765894 (-) 282 WP_235728219.1 DUF5658 family protein -
  ACK1LH_RS14205 (ACK1LH_14205) - 2766166..2766378 (-) 213 WP_029279723.1 hypothetical protein -
  ACK1LH_RS14210 (ACK1LH_14210) - 2766445..2766768 (+) 324 WP_029279721.1 DUF3889 domain-containing protein -
  ACK1LH_RS14215 (ACK1LH_14215) - 2766826..2767005 (-) 180 WP_029279719.1 YqzE family protein -
  ACK1LH_RS14220 (ACK1LH_14220) - 2767031..2767549 (-) 519 WP_029279717.1 shikimate kinase -
  ACK1LH_RS14225 (ACK1LH_14225) comGG 2767623..2767997 (-) 375 WP_029279716.1 competence type IV pilus minor pilin ComGG -
  ACK1LH_RS14230 (ACK1LH_14230) comGF 2767994..2768533 (-) 540 WP_267881654.1 competence type IV pilus minor pilin ComGF -
  ACK1LH_RS14235 (ACK1LH_14235) - 2768418..2768726 (-) 309 WP_029279712.1 hypothetical protein -
  ACK1LH_RS14240 (ACK1LH_14240) comGD 2768713..2769162 (-) 450 WP_029279711.1 competence type IV pilus minor pilin ComGD -
  ACK1LH_RS14245 (ACK1LH_14245) comGC 2769140..2769451 (-) 312 WP_029279709.1 competence type IV pilus major pilin ComGC -
  ACK1LH_RS14250 (ACK1LH_14250) comGB 2769470..2770504 (-) 1035 WP_411333451.1 competence type IV pilus assembly protein ComGB -
  ACK1LH_RS14255 (ACK1LH_14255) comGA 2770494..2771561 (-) 1068 WP_029279705.1 competence type IV pilus ATPase ComGA Machinery gene
  ACK1LH_RS14265 (ACK1LH_14265) - 2771958..2772206 (-) 249 WP_029279703.1 hypothetical protein -
  ACK1LH_RS14270 (ACK1LH_14270) - 2772389..2772769 (-) 381 WP_035207135.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACK1LH_RS14275 (ACK1LH_14275) - 2773040..2773285 (+) 246 WP_029279699.1 DUF2626 domain-containing protein -
  ACK1LH_RS14280 (ACK1LH_14280) - 2773340..2774428 (-) 1089 WP_411333452.1 class I SAM-dependent methyltransferase -
  ACK1LH_RS14285 (ACK1LH_14285) - 2774437..2775069 (-) 633 WP_203288084.1 MBL fold metallo-hydrolase -
  ACK1LH_RS14290 (ACK1LH_14290) - 2775151..2775597 (+) 447 WP_029279692.1 hypothetical protein -
  ACK1LH_RS14295 (ACK1LH_14295) - 2775681..2775854 (+) 174 WP_029279690.1 DUF2759 domain-containing protein -
  ACK1LH_RS14300 (ACK1LH_14300) - 2775918..2776226 (-) 309 WP_029279688.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39987.46 Da        Isoelectric Point: 7.6600

>NTDB_id=1082789 ACK1LH_RS14255 WP_029279705.1 2770494..2771561(-) (comGA) [Metabacillus indicus strain JKLC.Or24]
MNSIELLSERIVEEACELNASDIHFVPRETDAVIQYRIDDDLIEKRIIRKEVCGRIIAHFKFLASMDIGERRRPQNGSLA
LSNEAINVQLRMSTLPTVFEESLVIRILPNDCIPSVTKLSLFPQSAQKLLSFLNHSHGLMVFTGPTGSGKTTTLYSLIHY
AKKHFNRSIITLEDPVETKSSEVLQVQVNEKAGITYAVGLKAILRHDPDIIMVGEIRDRETAQIAIRAALTGHLVLSTMH
TRDAKGAIYRMLEFDVSLSEIEQTFLAVVAQRLVGVKCPFCEGECSPQCKKMRNVRRLGIFEMLYGKNLTLAIKEARGEK
VECDYATLKDAIKKGIALGYLPSYALHKWMYAYED

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=1082789 ACK1LH_RS14255 WP_029279705.1 2770494..2771561(-) (comGA) [Metabacillus indicus strain JKLC.Or24]
ATGAATTCCATCGAATTGCTCAGTGAAAGAATTGTTGAAGAAGCCTGTGAGCTTAACGCTTCTGATATTCATTTTGTTCC
CAGGGAAACAGACGCGGTCATTCAATACAGAATCGATGATGATCTCATTGAAAAAAGGATTATCCGCAAAGAGGTCTGCG
GCAGGATTATTGCCCATTTTAAATTTCTTGCCTCTATGGATATTGGCGAGAGAAGGAGGCCGCAAAACGGTTCACTTGCT
CTGTCAAATGAGGCAATCAATGTTCAGCTTCGCATGTCCACTCTTCCTACCGTATTTGAAGAAAGTCTAGTGATCAGAAT
CCTCCCGAATGATTGCATTCCTTCCGTTACCAAACTGTCGCTTTTTCCGCAGTCCGCACAAAAACTTCTCTCTTTTCTAA
ACCATTCCCACGGTTTAATGGTTTTTACGGGTCCAACCGGTTCCGGAAAAACAACGACTCTCTATTCTCTCATTCATTAT
GCCAAAAAACATTTTAACCGGAGCATTATAACGCTTGAGGATCCGGTTGAAACGAAAAGCTCCGAGGTTCTGCAGGTACA
GGTTAATGAAAAAGCGGGGATCACGTATGCGGTCGGGCTGAAGGCCATACTCAGGCACGACCCGGATATCATAATGGTCG
GTGAGATCAGAGACCGGGAAACTGCACAAATTGCAATCAGGGCAGCGCTCACAGGACATTTGGTTCTGTCGACGATGCAT
ACAAGAGATGCCAAAGGCGCGATTTACAGAATGCTTGAATTTGATGTAAGCCTGTCTGAAATTGAGCAGACCTTTCTAGC
TGTCGTTGCTCAGAGGCTTGTCGGTGTCAAATGTCCATTTTGCGAGGGCGAATGCTCACCGCAGTGTAAAAAAATGAGAA
ACGTCAGGAGACTTGGAATCTTTGAAATGCTGTACGGGAAAAACCTTACACTTGCCATAAAAGAAGCACGCGGGGAAAAG
GTCGAATGTGATTATGCCACCTTAAAGGATGCGATAAAGAAGGGGATTGCCCTGGGGTACTTGCCGTCTTATGCTCTTCA
TAAGTGGATGTATGCCTATGAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

63.202

100

0.634

Multiple sequence alignment