Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AACH71_RS07050 Genome accession   NZ_AP029050
Coordinates   1374351..1377851 (-) Length   1166 a.a.
NCBI ID   WP_136654706.1    Uniprot ID   -
Organism   Bacillus stercoris strain BST19     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1369351..1382851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH71_RS07040 (BSB_13880) sbcD 1369413..1370588 (-) 1176 WP_136654704.1 exonuclease subunit SbcD -
  AACH71_RS07045 (BSB_13890) addA 1370660..1374364 (-) 3705 WP_136654705.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AACH71_RS07050 (BSB_13900) addB 1374351..1377851 (-) 3501 WP_136654706.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AACH71_RS07055 (BSB_13910) - 1378035..1378472 (+) 438 WP_136654707.1 ferritin family protein -
  AACH71_RS07060 - 1378591..1378920 (+) 330 WP_014663456.1 four-helix bundle copper-binding protein -
  AACH71_RS07065 (BSB_13920) - 1378957..1380684 (-) 1728 WP_136654708.1 SgrR family transcriptional regulator -
  AACH71_RS07070 (BSB_13930) - 1380798..1382000 (+) 1203 WP_095432675.1 MFS transporter -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134630.51 Da        Isoelectric Point: 5.4728

>NTDB_id=108260 AACH71_RS07050 WP_136654706.1 1374351..1377851(-) (addB) [Bacillus stercoris strain BST19]
MGAEFLVGRSGSGKTKLIINSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRILQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKNGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLAEDVKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTQTPELAHLEAQYEARPAVPYAEKQEALTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLSEPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFQYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETEVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLFEEFVEMMGDDEI
SLDLFQQMIEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVVKRLEELFPHHKERLLTNEPEQVSDEEQLKYVVNQS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFQLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEVVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFDLLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRTLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=108260 AACH71_RS07050 WP_136654706.1 1374351..1377851(-) (addB) [Bacillus stercoris strain BST19]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCAACAGCATTCAGGATGAATTGCG
CCGGGCTCCATTCGGGAAGCCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCAGATATGGGCGGGATGATACGCGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCATCCTTCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTGACGAGCACCGGCGTACAAATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
CAAAGTCTATCAAAAAGCGAGTGACAAAAACGGGTTTACCGCACAGGTTGAGCGGATGCTGACAGAGTTTAAGCGGTATT
GTCTGGAACCGGAGGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAATATCGCGGAGAACGTGTGTTATCTGAA
AAGCTCCATGACTTATCGATTCTGTATCAGCAAATGGAAAAAAGCCTCGCAGATCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATCCCGCTTGCAGAAGATGTAAAAGGCGCGCATATCTATGTGGATGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGCTGGAGCAGCTTATGGTTCATGCAGAGCATATCACGTTTTCGCTCACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGAAAAACCTATTACCGCCTGCATCAGAAGGCGAA
GGAACTGAATTTAGACATTACGTATAAAGAGCTGAGCGGGACTGAGCGGCATACACAGACACCTGAATTGGCCCATCTAG
AGGCGCAGTATGAAGCGCGTCCTGCCGTTCCTTACGCAGAAAAACAAGAAGCACTGACTGTAATGCAGGCCGCGAACAGA
CGAGCTGAGCTGGAAGGTATTGCCCGGGAAATTCACGCCCTCGTCAGAGAGAAAGGATATCGATATAAGGATGTAGCAAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCATCCATTAATCGAATTTATCCGCTCAAGCCTTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTCCGCTGTGTGAAAACCGAACTGCTATTCCCGCTCAGTGAACCGAAGGCCAAAGTGAGAGAACAGGTCGA
TCAGCTCGAAAATTACTGTATCGCTTATGGGATCAAAGGCGACCGCTGGACAAAGGGCGACCGTTTCCAATATAGGCGCT
TTGTGTCATTGGATGATGATTTTGCGCAGACCGATCAGGAAATCGAAATGGAAAACATGTTGAATGACACCCGCGATTGG
ATTGTTCCGCCGCTTTTTCAGCTTCAAAAACGAATGAAAAAAGCAAAGACGGTACAGGAGAAGGCGGAGGCGCTCTATCG
CTATTTAGAAGAGACGGAGGTGCCGCTGAAGCTGGATCAGGAAAGGCAGCGTGCTGAGGAAGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGACGCAGTCATTCAGCTGTTTGAGGAGTTTGTTGAAATGATGGGAGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATAGAAGCCGGCGCTGAGTCGCTTACATTTTCTCTTATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACATCCTGCACCTTTGTGCTCGGGGCAAACGACGGCGTTC
TGCCGGCACGCCCTGATGAAAACGGGGTTCTGTCGGATGATGACCGGGAATGGCTGAAAACGATCGGGGTTGAGCTTTCC
TCAGGCGGACGGGAGCGTTTGCTTGATGAGCATTTCCTCATCTACATGGCATTTTCAAGTCCGTCTGACCGGCTTTATGT
ATCGTATCCGATTGCAGATGCGGAAGGAAAAACGCTTTTGCCGTCGATTGTCGTTAAACGGCTGGAAGAACTGTTTCCGC
ATCATAAGGAGCGCCTGTTGACAAATGAACCTGAGCAGGTCAGCGATGAGGAACAGCTGAAGTATGTTGTCAATCAAAGC
GTGGCACAGTCCTTTACTGCGAGTCAGCTCAGATTATGGACCCGGGAATATGACATCAGTGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTAAAGC
AGCTTGAACGCAGTGTGTCAAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCA
TGTCCGTTTTCTCATTTTGCGTCACACGGGCTGCATCTGAAGGAACGGCAATTTTTCAAGCTTGAAGCGCCGGATATTGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACAGGCTGCGTGAGCAAAAGCTGGATTGGCGAGATTTAACGAAAG
AGCAGTGCGAGCTGTTTTCTTATGATGCGGTAGAGCGGCTGGCACCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTAAAGGAAAAACTGCAAAAAATTGTGACGCGTGTGTCCGGTATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTACCGATCGGGCTTGAACTGGGCTTCGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTCAACTGAAAAACG
GCTGTACGATGGAGCTCGTCGGGCGTATTGACCGTGTGGATAAAGCCGAAAGCTCAAAAGGCCTTCTCCTCAGAATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGATTGGCGCTGCAAATGCTGACGTACCTTGA
CTTATCAATTACACATTCAGCTGACTGGCTCGGAATGAGGGCAACGCCTGCCGGAGTGCTGTATTTCCATATTCATGATC
CGATGATTCAATCTAATCTCCCGCTCGGGCTTGACGAGATTGAACAGGAGATTTTTAAGAAATTTAAAATGAAGGGCCTG
CTCCTCGGCGATCAGGAAGTGGTTCGCCTTATGGATACAACCCTTCAAGAGGGACGGTCAAATATCATCAACGCCGGCTT
GAAAAAAGACGGCTCTCTCAGATCAGACTCGGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCC
GTACCTTCCAAGAAGCAGGCGAACAAATTACCGACGGCCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACACCG
TGCACATACTGTGCGTTCAAATCAGTGTGCCAATTTGATGAATCACTAGAGGAAAACGAGTATCGCACATTAAAAGCTGA
AAAAGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(305-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

98.714

100

0.987

Multiple sequence alignment