Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   AACH71_RS07045 Genome accession   NZ_AP029050
Coordinates   1370660..1374364 (-) Length   1234 a.a.
NCBI ID   WP_136654705.1    Uniprot ID   -
Organism   Bacillus stercoris strain BST19     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1365660..1379364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH71_RS07030 (BSB_13860) hlpB 1365708..1366010 (-) 303 WP_136654702.1 HNH nuclease-like protein HlpB -
  AACH71_RS07035 (BSB_13870) sbcC 1366024..1369416 (-) 3393 WP_136654703.1 exonuclease subunit SbcC -
  AACH71_RS07040 (BSB_13880) sbcD 1369413..1370588 (-) 1176 WP_136654704.1 exonuclease subunit SbcD -
  AACH71_RS07045 (BSB_13890) addA 1370660..1374364 (-) 3705 WP_136654705.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AACH71_RS07050 (BSB_13900) addB 1374351..1377851 (-) 3501 WP_136654706.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AACH71_RS07055 (BSB_13910) - 1378035..1378472 (+) 438 WP_136654707.1 ferritin family protein -
  AACH71_RS07060 - 1378591..1378920 (+) 330 WP_014663456.1 four-helix bundle copper-binding protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141279.43 Da        Isoelectric Point: 4.9510

>NTDB_id=108259 AACH71_RS07045 WP_136654705.1 1370660..1374364(-) (addA) [Bacillus stercoris strain BST19]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVQRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIEHQDDFSELYRRVPAVSFKRAKAVKGDEFDPALLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLTEMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAQNDKGEYEPSEAARFYQEQ
FYEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETDTELLLIDNAEDTDASEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTDRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRSFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKNHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDLEALAGNGAPVHADISGHPARFAVQMIHSYD
LLDDDVEERMEEKSERLEAIRRGEPVPGSFAFDEKAREQLNWTYPHQEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLSHVPSIEEAEQTVQRLYEKELLTEEQKDAIDIEEIVQFFHTEIGG
QLIGAKWKDREIPFSLALPAKEIYPDAHEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYD
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=108259 AACH71_RS07045 WP_136654705.1 1370660..1374364(-) (addA) [Bacillus stercoris strain BST19]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
CGTGGCAGCGGCTGCGGGCTCTGGTAAAACCGCTGTGCTCGTTGAACGAATGATTCGGAAAATCACCGCAGAGGAAAACC
CAATTGATGTAGACCGCCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGAATTGCGGATGCC
TTGGAAAAAGAGCTTGTACAACGCCCCGGCTCGCTGCATATTAGACGCCAGCTTTCTCTTTTGAACCGAGCCAGCATTTC
GACGCTTCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATAGCTG
ATCAGACGGAAGGCGAGCTGCTTGGGGATGAAGTGCTTGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAAAAGGCA
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCACGATCTGGATCTGCAATTTCTCGTTAAACAAGTGTACGAGTA
TTCCCGATCCCATCCCAACCCGGAGGCGTGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCAGAAAAGAGCGCCATCG
AGGAGCTGCCGTTTTATCAATATGTCAAAGAGGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGACCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATCGAGCATCAGGACGATTTTAGTGAACTATATCGTCGTGTGCCCGCCGTCTCCTTTAAGCGTGCCAAAGCAGTAAAAG
GGGATGAGTTCGATCCGGCGCTCCTTGATGAGGCGACAGATTTGAGAAACGGCGCAAAAAAACTGCTCGAAAAGCTCAAA
ACCGACTACTTTACGAGAAGTCCTGAACAGCACTTGAAAAGCCTAACCGAGATGAAGCCTGTCATTGAAACGCTCGTACA
GCTTGTTATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATCGATTTTTCTGATTTGGAGCATT
ACTGTTTAGCGATTTTGACAGCTCAGAATGACAAAGGTGAATATGAGCCGAGCGAGGCTGCAAGGTTTTATCAGGAGCAG
TTTTATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTCGTACAGGAATCGATTCTGCAGCTCGTCACAAGCGGGCC
GGAGGAGACTGGCAACCTGTTTATGGTAGGCGATGTCAAACAGTCAATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCTAAATACAAACGGTTTACAGAGAGCGGGGAAGGCACGGGACGGAAAATCGACTTGAATAAAAATTTCCGAAGC
CGTTCAGATATATTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTCGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGACAATGACGAGACGGATACGGAGCTGCTGCTGATCGACAACGCAG
AAGATACGGATGCAAGCGAGGAAGCAGAAGAGCTTGAAACCGTGCAGTTTGAGGCCAAAGCCATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACAGATCGCAATATTCAATACCGAGATATTGTGAT
TTTGCTCCGCTCCATGCCGTGGGCTCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTCGAAGCGGTCGAAGTCGCCGTCGCGCTTTCAGTGCTGAAGGTGATCGATAATCCGTATCAGGATATA
CCGCTTGCCTCTGTGCTGCGTTCACCGATTGTCGGTGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATACAAA
AGCGCCTTACTATGAAGCGATGAAAGACTACCTGGCTGCTGGTGACCGGAACGACGAGCTTTATCAAAAGCTGCAAACGT
TCTACGGACATCTGCAAAAATGGCGCTCTTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGACTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTTTATGACAGGGC
GCGCCAATATGAATCAACGGCATTTCGCGGCTTGTTTCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTTGCAGCGGGTCTCGGCCGGAATTTTAACATGATGGATTTAAATAAATCGTACCTGCTGGACAAGGA
GCTCGGATTCGGCACGAAGTATATTCATCCGCAATTGCGCATCAGCTACCCGACGCTGCCTCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCGTGCAAGAATCATCAGAAACAGCTCGCAAAATGGCAGGCATCCGCGTCCCAAACTGATTGGCTCCTTCCGGA
ATTTGACCGCTATCAGGCGAAAACGTATTTAGATTTCATCGGACCGGCTCTTGCCAGACATAGAGACTTGGAGGCTTTGG
CTGGAAACGGTGCACCAGTACACGCTGATATCTCAGGCCATCCAGCTCGTTTTGCCGTTCAAATGATTCATTCCTATGAT
TTGCTTGATGATGATGTGGAAGAAAGAATGGAAGAAAAAAGTGAACGTCTGGAAGCGATCCGCCGAGGGGAACCAGTTCC
CGGCTCGTTTGCATTTGATGAAAAAGCCCGCGAGCAGCTGAACTGGACCTACCCGCATCAAGAGGTGACACAAATTCGGA
CAAAGCAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCTGTAAAACCGGCT
GACGGAAGCATTCTGTACAGACGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCAGAGAAAGGTACTGCCATGCA
TACGGTGATGCAGCATATCCCGTTGTCACATGTGCCGTCGATAGAAGAAGCTGAGCAGACGGTTCAGAGGCTTTATGAAA
AAGAGCTGCTTACTGAAGAACAAAAAGACGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGGGGA
CAGCTGATCGGTGCGAAATGGAAGGACCGGGAAATACCTTTCAGCTTGGCGCTCCCGGCTAAGGAGATCTATCCTGATGC
ACACGAGGCGGATGAGCCGCTTTTAGTGCAGGGGATCATTGACTGTCTCTATGAAACTGAGGACGGATTATATTTATTGG
ATTATAAGTCGGACCGGATTGAGGGCAAATTCCAACACGGATTTGAAGGAGCAGCCCCGATCTTGAAGAAACGATACGAT
ACCCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTCACATTATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

97.65

100

0.977

Multiple sequence alignment