Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACK2V4_RS13305 Genome accession   NZ_CP176646
Coordinates   2866263..2866841 (-) Length   192 a.a.
NCBI ID   WP_062855254.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain GTVSS-031     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2861263..2871841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2V4_RS13280 (ACK2V4_13280) aroB 2861553..2862653 (-) 1101 WP_062855255.1 3-dehydroquinate synthase -
  ACK2V4_RS13285 (ACK2V4_13285) aroK 2862685..2863203 (-) 519 WP_005381319.1 shikimate kinase AroK -
  ACK2V4_RS13290 (ACK2V4_13290) pilQ 2863392..2865140 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  ACK2V4_RS13295 (ACK2V4_13295) pilP 2865172..2865687 (-) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  ACK2V4_RS13300 (ACK2V4_13300) pilO 2865677..2866270 (-) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACK2V4_RS13305 (ACK2V4_13305) pilN 2866263..2866841 (-) 579 WP_062855254.1 PilN domain-containing protein Machinery gene
  ACK2V4_RS13310 (ACK2V4_13310) pilM 2866825..2867841 (-) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  ACK2V4_RS13315 (ACK2V4_13315) - 2867984..2870554 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -
  ACK2V4_RS13320 (ACK2V4_13320) oxyR 2870610..2871518 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22420.92 Da        Isoelectric Point: 6.9944

>NTDB_id=1081932 ACK2V4_RS13305 WP_062855254.1 2866263..2866841(-) (pilN) [Vibrio parahaemolyticus strain GTVSS-031]
MLYRINLLPWRENQREEHRRRFVGLVVLGVLVALGIQWGVGKYYEYQQDLQQERLDYLTTYISDLDKRIEAMKIAELEHS
KILERLKVVEALQNGRNKTTDFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKARFGKEFQTFKVSFMFNTVEEKKEGKQHG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=1081932 ACK2V4_RS13305 WP_062855254.1 2866263..2866841(-) (pilN) [Vibrio parahaemolyticus strain GTVSS-031]
GTGCTGTATCGCATTAACTTATTGCCTTGGCGCGAAAATCAAAGGGAAGAGCATCGTCGTCGATTTGTCGGTTTAGTGGT
ATTGGGCGTCTTGGTGGCGTTGGGGATTCAGTGGGGCGTTGGCAAATATTATGAGTACCAACAAGATCTTCAACAAGAGC
GACTCGACTATCTAACAACGTACATTTCCGATCTAGATAAGCGGATAGAAGCGATGAAGATCGCGGAGCTTGAACACAGC
AAAATCTTGGAACGCTTGAAAGTTGTGGAAGCGTTGCAAAATGGTCGGAATAAAACCACTGATTTTATGAACCTCATGCC
TGCTGTCATTCCAGAGGGCGTGTATGTCGACAAGATCAAAATGAACGATCTAGAAATTGAAATCTCAGGTATTTCTGACA
GTACACCACGGCTAGCAACCATGCTCGATAACATGGAACGTTCTGCCAAGCTACTGGACGTTGAAATGCACTCTATCGTG
CACGGAAAAGCCCGTTTTGGTAAAGAGTTTCAGACCTTTAAAGTGTCCTTCATGTTTAACACGGTAGAAGAGAAGAAAGA
GGGAAAACAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

83.333

100

0.833
  pilN Vibrio cholerae strain A1552

63.536

94.271

0.599

Multiple sequence alignment