Detailed information NTG1081933

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACK2V4_RS13310
• Genome accession: NZ_CP176646
• Coordinates: 2866825..2867841 (-)
• Length: 338 a.a.
• NCBI ID: WP_005496568.1
• Uniprot ID: A0A3E1IL27
• Organism: Vibrio parahaemolyticus strain GTVSS-031
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2861825..2872841

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK2V4_RS13285 (ACK2V4_13285)	aroK	2862685..2863203 (-)	519	WP_005381319.1	shikimate kinase AroK	-
ACK2V4_RS13290 (ACK2V4_13290)	pilQ	2863392..2865140 (-)	1749	WP_005488227.1	type IV pilus secretin PilQ	Machinery gene
ACK2V4_RS13295 (ACK2V4_13295)	pilP	2865172..2865687 (-)	516	WP_005454781.1	pilus assembly protein PilP	Machinery gene
ACK2V4_RS13300 (ACK2V4_13300)	pilO	2865677..2866270 (-)	594	WP_005496571.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACK2V4_RS13305 (ACK2V4_13305)	pilN	2866263..2866841 (-)	579	WP_062855254.1	PilN domain-containing protein	Machinery gene
ACK2V4_RS13310 (ACK2V4_13310)	pilM	2866825..2867841 (-)	1017	WP_005496568.1	type IV pilus biogenesis protein PilM	Machinery gene
ACK2V4_RS13315 (ACK2V4_13315)	-	2867984..2870554 (+)	2571	WP_005496567.1	penicillin-binding protein 1A	-
ACK2V4_RS13320 (ACK2V4_13320)	oxyR	2870610..2871518 (-)	909	WP_005465160.1	DNA-binding transcriptional regulator OxyR	-
ACK2V4_RS13325 (ACK2V4_13325)	-	2871689..2872417 (+)	729	WP_015297397.1	redoxin family protein	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37762.28 Da
• Isoelectric Point: 6.8323

>NTDB_id=1081933 ACK2V4_RS13310 WP_005496568.1 2866825..2867841(-) (pilM) [Vibrio parahaemolyticus strain GTVSS-031]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide

• Length: 1017 bp

>NTDB_id=1081933 ACK2V4_RS13310 WP_005496568.1 2866825..2867841(-) (pilM) [Vibrio parahaemolyticus strain GTVSS-031]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ATCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-326)

3D structure

• AlphaFold DB: A0A3E1IL27

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio campbellii strain DS40M4	85.207	100	0.852
pilM	Vibrio cholerae strain A1552	48.368	99.704	0.482