Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACK2JB_RS07865 Genome accession   NZ_CP176630
Coordinates   1944715..1945647 (-) Length   310 a.a.
NCBI ID   WP_016475229.1    Uniprot ID   -
Organism   Sutterella wadsworthensis strain SFAD58     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1939715..1950647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2JB_RS07840 trmL 1939800..1940270 (-) 471 WP_411354096.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  ACK2JB_RS07845 rpoH 1940344..1941249 (-) 906 WP_081454003.1 RNA polymerase sigma factor RpoH -
  ACK2JB_RS07850 - 1941582..1942490 (-) 909 WP_005429352.1 cell division protein FtsX -
  ACK2JB_RS07855 - 1942487..1943182 (-) 696 WP_005429353.1 cell division ATP-binding protein FtsE -
  ACK2JB_RS07860 - 1943195..1944664 (-) 1470 WP_016475230.1 Ppx/GppA phosphatase family protein -
  ACK2JB_RS07865 pilA 1944715..1945647 (-) 933 WP_016475229.1 signal recognition particle-docking protein FtsY Machinery gene
  ACK2JB_RS07870 rsmD 1945695..1946399 (+) 705 WP_236578873.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACK2JB_RS07875 coaD 1946477..1946968 (+) 492 WP_005429366.1 pantetheine-phosphate adenylyltransferase -
  ACK2JB_RS07880 - 1947024..1949210 (-) 2187 WP_005429367.1 hypothetical protein -

Sequence

Protein


Download         Length: 310 a.a.        Molecular weight: 33053.82 Da        Isoelectric Point: 5.1145

>NTDB_id=1081656 ACK2JB_RS07865 WP_016475229.1 1944715..1945647(-) (pilA) [Sutterella wadsworthensis strain SFAD58]
MALGSSEGWFSRLKNGLSRTRVNIVGLFSGGVVDEDFLEELEFALISADVGVETTTHILERLRSDIKLQGLKTQEEVKLA
LRDELVTILKPAEGVLNVDQAKPCVIMMCGVNGAGKTTSIGKLAKRFADNGKSVLLAAGDTFRAAAREQLAVWGERNRVE
VIAQTGGDPAAVVFDAVNAGRARSMDVVIADTAGRLPTQTNLMEELSRIRRAQEKALEGAPHEVILVVDGTNGQNALAQV
AAFDTYAHLTGLIVTKLDGTAKGGVLAAITAARGERRLPIYYIGVGEKLEDLQPFSAEEFANALVGLDAK

Nucleotide


Download         Length: 933 bp        

>NTDB_id=1081656 ACK2JB_RS07865 WP_016475229.1 1944715..1945647(-) (pilA) [Sutterella wadsworthensis strain SFAD58]
ATGGCTTTGGGTTCTTCTGAGGGTTGGTTTTCCCGTTTGAAAAATGGGCTTTCACGCACCCGCGTCAATATCGTCGGACT
TTTTTCAGGCGGCGTCGTTGACGAGGATTTTCTCGAGGAGCTGGAGTTTGCGCTGATCTCCGCTGATGTCGGCGTAGAGA
CAACAACGCATATCTTAGAGCGCCTTCGCAGCGATATTAAGCTCCAAGGATTGAAGACTCAGGAAGAGGTGAAACTCGCG
CTGCGTGATGAGCTGGTCACCATTCTCAAACCTGCTGAAGGCGTACTCAACGTTGATCAAGCGAAGCCTTGCGTCATCAT
GATGTGCGGTGTGAACGGTGCTGGTAAAACGACTAGTATCGGTAAACTTGCCAAACGTTTCGCCGACAACGGCAAGAGCG
TCCTTTTAGCCGCCGGTGATACTTTCCGTGCTGCCGCACGGGAGCAGCTCGCCGTCTGGGGTGAACGCAACCGCGTCGAA
GTTATTGCACAGACTGGAGGTGATCCGGCAGCGGTGGTTTTTGATGCCGTCAATGCCGGCCGGGCCCGCAGCATGGATGT
GGTTATCGCCGATACAGCGGGACGACTTCCTACGCAGACCAATCTGATGGAAGAACTCTCGCGCATCCGCCGCGCTCAAG
AAAAAGCGCTCGAAGGTGCGCCGCATGAGGTGATTCTCGTGGTAGACGGTACAAACGGCCAAAATGCATTGGCGCAGGTG
GCCGCGTTCGACACTTACGCTCACCTTACCGGTCTGATCGTTACCAAATTGGATGGCACGGCCAAAGGCGGCGTTCTTGC
AGCCATTACAGCTGCCCGAGGAGAGCGGAGACTGCCCATTTACTATATCGGCGTAGGCGAAAAACTCGAAGACCTGCAGC
CTTTCAGTGCTGAAGAATTTGCCAATGCGCTGGTTGGTCTGGACGCAAAATAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(26-85)

SRP54-type protein, GTPase domain (SRP54)

(104-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

56.106

97.742

0.548

Multiple sequence alignment