Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   ACK3CJ_RS05050 Genome accession   NZ_CP176580
Coordinates   1083655..1085073 (+) Length   472 a.a.
NCBI ID   WP_409525337.1    Uniprot ID   -
Organism   Nitrincola sp. WZQS7-5     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1085887..1100111 1083655..1085073 flank 814


Gene organization within MGE regions


Location: 1083655..1100111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK3CJ_RS05050 pilR 1083655..1085073 (+) 1419 WP_409525337.1 sigma-54-dependent transcriptional regulator Regulator
  ACK3CJ_RS05055 comP 1085428..1085826 (+) 399 WP_409525338.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACK3CJ_RS05060 - 1085887..1087818 (+) 1932 WP_409525339.1 hypothetical protein -
  ACK3CJ_RS05065 - 1087802..1088686 (+) 885 WP_409525340.1 glycosyltransferase family 2 protein -
  ACK3CJ_RS05070 - 1088703..1089482 (-) 780 WP_409525342.1 cephalosporin hydroxylase family protein -
  ACK3CJ_RS05075 - 1089490..1090182 (-) 693 WP_409525343.1 metallophosphoesterase family protein -
  ACK3CJ_RS05080 - 1090163..1091161 (-) 999 WP_409525344.1 ATP-grasp domain-containing protein -
  ACK3CJ_RS05085 - 1091158..1092039 (-) 882 WP_409525345.1 NAD-dependent epimerase/dehydratase family protein -
  ACK3CJ_RS05090 - 1092036..1092758 (-) 723 WP_409525346.1 class I SAM-dependent methyltransferase -
  ACK3CJ_RS05095 - 1092748..1093497 (-) 750 WP_409525347.1 WbqC family protein -
  ACK3CJ_RS05100 - 1093508..1094632 (-) 1125 WP_409525348.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  ACK3CJ_RS05105 - 1094629..1095852 (-) 1224 WP_409525350.1 methyltransferase domain-containing protein -
  ACK3CJ_RS05110 - 1095849..1096412 (-) 564 WP_409525351.1 dTDP-4-dehydrorhamnose 3,5-epimerase family protein -
  ACK3CJ_RS05115 rfbG 1096405..1097469 (-) 1065 WP_409525352.1 CDP-glucose 4,6-dehydratase -
  ACK3CJ_RS05120 rfbF 1097478..1098251 (-) 774 WP_409525353.1 glucose-1-phosphate cytidylyltransferase -
  ACK3CJ_RS05125 - 1098308..1099219 (-) 912 WP_409525354.1 hypothetical protein -
  ACK3CJ_RS05130 - 1099206..1100111 (-) 906 WP_409525355.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 472 a.a.        Molecular weight: 52445.92 Da        Isoelectric Point: 5.1744

>NTDB_id=1081050 ACK3CJ_RS05050 WP_409525337.1 1083655..1085073(+) (pilR) [Nitrincola sp. WZQS7-5]
MSDNKATRRVLIVDDEPDIRELLGITLSRMDITCTEAGDLQQARDALSRDTFQLCLVDMRLPDGNGIDLIRHIQRTRPNL
PVAMITAHGNMQSAIDALKAGAFDFLNKPVELPTLRNLVRSALLLGEQAESDEVPASNDCLQLLGRSAPIESIRHTIRKL
ARSQAPVYISGESGTGKELVARLIHAQGPRAEQPFVAVNCGAIPAELMESELFGYKKGSFTGAYADKDGLFQSADGGTLF
LDEVADLPLGMQVKLLRAIQEKVVRPVGSQTEVHVDVRILSATHKDLSQQVAEKRFREDLYYRINVIELHVPSLRERPED
IPLLADHFIHYLAQKMEIQVPELSAGARSELHQYSFPGNVRQLENILERALTLCEGAVIEADDLLLDKNNHHEQPPITPA
AQLISSPFDLDELIPDEALVEQVGLEAYMESIERKLINDALQSSGHNKTAAAKRLGISFRALRYRLKKLEME

Nucleotide


Download         Length: 1419 bp        

>NTDB_id=1081050 ACK3CJ_RS05050 WP_409525337.1 1083655..1085073(+) (pilR) [Nitrincola sp. WZQS7-5]
ATGAGTGATAACAAAGCAACCAGACGTGTACTGATTGTGGATGATGAGCCTGATATCCGCGAACTGCTGGGTATCACCTT
GTCCCGTATGGATATCACCTGCACCGAAGCTGGCGACTTGCAGCAGGCCAGAGACGCGCTGAGTCGTGACACCTTTCAGT
TGTGTCTGGTGGATATGCGTCTGCCGGATGGCAACGGCATTGATCTTATCCGCCATATCCAACGCACGCGGCCTAATCTA
CCGGTCGCGATGATTACCGCGCATGGCAATATGCAATCCGCTATTGATGCACTCAAAGCGGGAGCGTTTGATTTTCTCAA
CAAGCCTGTTGAACTGCCCACGCTGAGAAACCTGGTTCGCTCAGCACTGCTGCTGGGTGAGCAGGCAGAGTCAGACGAAG
TTCCAGCCAGTAATGATTGCTTGCAACTGCTTGGCCGCTCTGCACCGATCGAATCGATACGTCATACCATCCGCAAACTG
GCGCGCAGCCAGGCACCTGTCTATATCAGCGGCGAGTCAGGCACAGGTAAAGAACTGGTAGCGCGCCTGATACATGCACA
AGGGCCACGCGCAGAGCAGCCATTTGTGGCCGTGAACTGCGGAGCGATTCCCGCTGAACTGATGGAAAGCGAACTTTTCG
GATACAAAAAAGGCAGCTTTACCGGTGCCTATGCGGATAAGGATGGGTTATTCCAGAGTGCAGACGGCGGCACTTTGTTT
CTGGATGAGGTCGCCGACCTGCCGCTGGGCATGCAGGTTAAACTCCTGCGCGCGATACAGGAAAAGGTGGTGCGACCAGT
TGGATCGCAGACGGAGGTGCATGTCGATGTGCGCATATTGAGCGCTACGCATAAGGATCTGTCGCAACAGGTAGCGGAAA
AGCGCTTTCGTGAGGATCTGTATTATCGGATCAATGTGATAGAGCTGCATGTGCCGTCGTTAAGAGAGCGCCCAGAAGAT
ATACCGCTGCTGGCGGATCACTTTATTCATTACCTTGCACAAAAAATGGAGATACAAGTACCGGAGCTGAGTGCGGGTGC
CAGAAGTGAGCTACACCAATACAGCTTCCCGGGTAATGTCCGACAGCTCGAGAACATCCTGGAGCGAGCCTTGACACTCT
GCGAAGGCGCAGTGATAGAGGCAGACGACCTGTTGCTTGATAAGAATAACCATCATGAACAGCCACCCATCACACCAGCA
GCACAACTGATCAGCAGCCCTTTTGATCTGGATGAGTTGATCCCGGATGAGGCGCTGGTGGAGCAGGTCGGCCTTGAGGC
TTATATGGAGAGCATCGAGCGCAAGCTGATTAATGATGCCTTGCAAAGCAGTGGCCACAACAAAACAGCAGCAGCCAAAC
GGCTGGGCATCTCATTTCGTGCGTTGCGCTACCGGCTGAAAAAGTTGGAGATGGAGTAA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(10-119)

Sigma-54 interaction domain (Sigma54_activat)

(144-309)

Bacterial regulatory protein, Fis family (HTH_8)

(429-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

59.915

99.364

0.595
  pilR Acinetobacter baumannii strain A118

49.356

98.729

0.487

Multiple sequence alignment