Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ACKZ7U_RS08355 Genome accession   NZ_CP176568
Coordinates   1674722..1675219 (-) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain IMT39173     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1669722..1680219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKZ7U_RS08330 (ACKZ7U_08330) gcvPB 1669884..1671356 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  ACKZ7U_RS08335 (ACKZ7U_08335) gcvPA 1671349..1672695 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ACKZ7U_RS08340 (ACKZ7U_08340) gcvT 1672715..1673806 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  ACKZ7U_RS08345 (ACKZ7U_08345) - 1673965..1674489 (-) 525 WP_001015121.1 shikimate kinase -
  ACKZ7U_RS08350 (ACKZ7U_08350) - 1674479..1674625 (-) 147 WP_001789879.1 hypothetical protein -
  ACKZ7U_RS08355 (ACKZ7U_08355) comGF 1674722..1675219 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACKZ7U_RS08360 (ACKZ7U_08360) comGE 1675137..1675436 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACKZ7U_RS08365 (ACKZ7U_08365) comGD 1675423..1675869 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACKZ7U_RS08370 (ACKZ7U_08370) comGC 1675847..1676158 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACKZ7U_RS08375 (ACKZ7U_08375) comGB 1676172..1677242 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACKZ7U_RS08380 (ACKZ7U_08380) comGA 1677214..1678188 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACKZ7U_RS08385 (ACKZ7U_08385) - 1678240..1678863 (-) 624 WP_001223010.1 MBL fold metallo-hydrolase -
  ACKZ7U_RS08390 (ACKZ7U_08390) - 1678860..1679189 (-) 330 WP_148844373.1 MTH1187 family thiamine-binding protein -
  ACKZ7U_RS08395 (ACKZ7U_08395) - 1679189..1680175 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=1080982 ACKZ7U_RS08355 WP_001789864.1 1674722..1675219(-) (comGF) [Staphylococcus aureus strain IMT39173]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1080982 ACKZ7U_RS08355 WP_001789864.1 1674722..1675219(-) (comGF) [Staphylococcus aureus strain IMT39173]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment